Attenuation of renal ischemic reperfusion injury by salvianolic acid B via suppressing oxidative stress and inflammation through PI3K/Akt signaling pathway

Abstract

Salvianolic acid B (SAB) is one the major phytocomponents of Radix Salvia miltiorrhiza and exhibit numerous health promoting properties. The objective of the current study was to examine whether SAB exerts a renoprotective effect by attenuating oxidative stress and inflammatory response through activating phosphatidylinositol 3-kinase/serine-threonine kinase B (PI3K/ Akt) signaling pathway in a renal ischemic reperfusion rat model. Forty Sprague-Dawley male rats (250-300 g) were obtained and split into four groups with ten rats in each group. The right kidney of all rats was removed (nephrectomy). The rats of the Control group received only saline (occlusion) and served as a sham control group, whereas rats subjected to ischemic reperfusion (IR) insult by clamping the left renal artery served as a postitive control group. The other 2 groups of rats were pretreated with SAB (20 and 40 mg Á kg -1 Á day -1 ) for 7 days prior IR induction and served as treatment groups (SAB 20+IR; SAB 40+IR). Renal markers creatinine (Cr) and blood urea nitrogen (BUN) were significantly lower in the groups that received SAB. Pretreatment with SAB appears to attenuate oxidative stress by suppressing the production of lipid peroxidation products like malondialdehyde as well as elevating antioxidant activity. The concentration of inflammatory markers and neutrophil infiltration (myeloperoxidase) were significantly decreased. Meanwhile, PI3K protein expression and pAkt/Akt ratio were significantly upregulated upon supplementation with SAB, indicating its renoprotective activity. Taken together, these results indicate that SAB can therapeutically alleviate oxidative stress and inflammatory process via modulating PI3K/Akt signaling pathway and probably ameliorate renal function and thus act as a renoprotective agent.

Salvianolic acid B abolished chronic mild stress‐induced depression through suppressing oxidative stress and neuro‐inflammation via regulating NLRP3 inflammasome activation

“…SB abolish CMS‐induced hyperactivity of HPA axis, which is confirmed by decreased ACTH and CORT serum level after 3 weeks of SB and IMP treatment. The above results showcase that SB can positively regulate the HPA axis owing to antioxidant, neuroprotective activities of SB (Lin et al, ; Ma et al, ).…”

“…Salvianolic acid B (SB) is one of the active components of Salvia militiorrhiza Bunge, a famous Chinese herb, which is now popular functional food in Asia owing to its various biological properties (Liu, Wu et al, ). SB display array of biological activities including anti‐inflammatory, antioxidant, anti‐apoptotic, anti‐microbial properties as well as cardioprotective, renoprotective, and hepatoprotective action (Lin et al, ; Ma, Xia, Cui, & Yu, ). Previously, Lee and his co‐workers () have revealed the neuroprotective activity of salvianolic acid B on Alzheimer's induced mouse model as it crosses the blood–brain barrier.…”

Retracted Article: Salvianolic acid B inhibits inflammatory response and cell apoptosisviathe PI3K/Akt signaling pathway in IL-1β-induced osteoarthritis chondrocytes

“…21,36 Ma et al have proved that Sal B inhibited the inammatory response and affected the prognosis of renal ischemia-reperfusion through PI3K/Akt signaling pathway. 37 In this study, we rst examined the toxicity of Sal B on human primary chondrocytes and showed that the cytotoxicity of chondrocytes when Sal B reached dose of 150 M. Aer the study, we found that Sal B reduced the upregulation of iNOS induced by IL-1b at safe range, reducing the secretion of NO in cartilage cells, reducing the mRNA levels of IL-6, IL-17 and TNF-a, thus inhibiting the inammation caused by IL-1b. This results are consistent with the Li et al and others published researches.…”

Cardioprotective effect of salvianolic acid B against isoproterenol-induced inflammation and histological changes in a cardiotoxicity rat model

“…Salvia militiorrhiza is a popular Chinese herb used in Traditional Chinese Medicine (TCM) for treating various ailments such as cerebrovascular and cardiovascular disease, and dysmenorrhea [12,13]. Salvianolic acid (SAB-B) exhibits a broad range of biological activities including anti-inflammatory, anti-apoptotic, antioxidant, anti-cancer and anti-diabetic, cardioprotective, neuroprotective, gastro-protective and renoprotective properties in various experimental models [14][15][16].…”