Introduction: Since prescribing is a new subject in the nursing profession, it is necessary to assess nurses' attitudes to and readiness for this new responsibility. Objective: This study aimed to investigate nurses' attitudes to and readiness for nurse prescribing in teaching hospitals of Tabriz City, Iran. Materials and Methods: This cross-sectional analytical study was conducted on 335 nurses working in the hospitals of Tabriz from June to October 2019. The participants were selected with a stratified random sampling method, and study data were collected using a two-part questionnaire: demographics and the nurse prescribing questionnaire.The demographics were analyzed using descriptive statistics. The Spearman rank correlation coefficient and the Mann-Whitney U tests were employed to examine the correlation and to compare the mean scores, respectively. The significance level for all tests was determined to be less than 0.05. Results: About 82.1% of participants were female, 59.7% were working in general wards, and 42.1% were in critical care units. The results showed that the mean ±SD scores of attitude and readiness were 34.79 ±8.15 and 36.99 ±6.01, respectively. Moreover, 71.6% of nurses agreed with nurse prescribing. The results showed that the score of attitudes towards prescribing the drug was significant in terms of gender (P = 0.039), employment status (P = 0.032), work experience (P =0.041), and academic degree (P = 0.028). Also, results showed readiness to implement was significant in terms of gender (P = 0.028), employment status (P = 0.049), work experience (P = 0.037), and academic degree (P = 0.042). Finally, there was a positive and significant correlation between nurses' attitudes toward and readiness for prescribing (P = 0.042, rs=0.626). Conclusion: Because nurses have a positive attitude toward and are ready for prescribing, it is possible to make them more prepared for this new role by increasing their pharmacological knowledge and improving their accountability.