The aim of this paper is to assess the accessibility of flagship cultural attractions—that is, museums located in Krakow, a city with a rich historical heritage included in the UNESCO World Cultural and Natural Heritage List and distinguished by being a recipient of the Access City Award—for people with disabilities. This research shows the degree to which these museums have been adapted to receive visitors with disabilities. Assessments were also conducted with regard to access to the museums’ websites and the possibility for virtual tours of their collections. Out of a total of 50 museums, 31 were selected for this study, both leading museums in terms of attendance, such as Wawel Royal Castle, the National Museum, the Museum of Krakow, and the Wieliczka Salt Mine, and smaller ones such as the Aviation Museum. The research results indicate that Krakow museums are well-prepared for receiving people with physical disabilities and seniors but are less well adapted to receiving visually impaired and blind people and hearing-impaired and deaf people. Analysis of the museums’ websites showed that they are well-prepared in terms of compatibility and comprehensibility but that they received worse assessment scores with regard to their perceivability and functionality. Visitors to the museums are principally adults, adolescents, and seniors. People with disabilities make up 10% of all visitors.