The main objectives of this research were twofold: firstly, to evaluate tourists’ perception of Gornje Podunavlje Special Nature Reserve, one of the four significant protected areas within the Serbian part of Mura-Drava-Danube Biosphere Reserve, and secondly, to identify the motivations behind tourists’ visits to wetlands and their overall satisfaction with the visits. In addition, this study was also focused on the relationships between aforementioned constructs and tourists’ socio-demographic characteristics and visit characteristics. Finally, this study attempts to address the relationships between tourists’ motives for their visit, their image perception and satisfaction. The research findings showed that each of the three remaining factors had significant relationships with Satisfaction and experience. The research was conducted in the form of the survey in the period between May 2020 and April 2022 on the sample of 216 visitors. The research findings showed that each of three remaining factors had significant relationships with Satisfaction and experience. Relationships are also found between identified improvement requirements, Motivation for spending time particularly in wetlands, and perceived biodiversity image. Finally, there are significant relationships between duration of the visit with Satisfaction and experience and Motivation for spending time in nature and future behavior. Practical implications of the research results might be beneficial for managers of wetland destinations, in order to improve the offer in line with tourists’ preferences.