Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor

Palacio, Irene; Moreno, Miguel; Náñez, Almudena; Purwidyantri, Agnes; Domingues, Telma; Cabral, Patrícia D.; Borme, Jérôme; Marciello, Marzia; Mendieta‐Moreno, Jesús I.; Torres-Vázquez, Beatriz; Martínez, José I.; López, María Francisca; Garcia-Hernandez, M.; Vázquez, Luís; Jelı́nek, Pavel; Alpuim, P.; Briones, Carlos; Martín‐Gago, José A.

doi:10.1016/j.bios.2022.115006

Cited by 21 publications

(13 citation statements)

References 38 publications

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“…Advances in GFET protein sensors have also focused on improving sensor performance with emerging technologies. [ 15,114 ] For example, Dai et al. developed a multiantibody functionalization strategy to enhance the sensitivity and accuracy of GFET biosensors.…”

Section: Biomarker Analysismentioning

confidence: 99%

“…[206][207][208] The primary step for epidemic prevention and control is rapid and accurate diagnosis of presymptomatic and asymptomatic individuals who have low viral loads. [208][209][210] This section summarizes the notable progress in GFET-based POC systems for sensitively detecting viruses such as Zika, [148] Ebola, [190] SARS-CoV-2, [142] hepatitis C, [114] tuberculosis, [77] and others. [211] Early advances have been made in antibody-modified GFET biosensors for amplification-free detection of infectious viruses in unprocessed samples.…”

Section: Infectious Virusmentioning

confidence: 99%

“…[50] Advances in GFET protein sensors have also focused on improving sensor performance with emerging technologies. [15,114] For example, Dai et al developed a multiantibody functionalization strategy to enhance the sensitivity and accuracy of GFET biosensors. In this configuration, three differential antibodies were immobilized on the graphene surface to bind not only RBD domains but also adjacent sites of SARS-CoV-2 (Figure 5a).…”

Section: Proteinmentioning

confidence: 99%

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Graphene Transistors for In Vitro Detection of Health Biomarkers

Dai

Kong

Chen

et al. 2023

Adv Funct Materials

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Biomarkers are primary indicators for precise diagnosis and treatment. The early identification of health biomarkers has been sustained by the evolutionary success in sensor technologies. Among them, graphene field-effect transistor (GFET) biosensors have exhibited major advantages such as an ultrashort response time, high sensitivity, easy operation, capability of integration, and label-free detection. Owing to the atomic thickness, graphene restricts charge carrier flow merely at the material surface and responds to foreign stimuli directly, leading to effective signal acquisition and transmission. Here, this review summarizes the latest advances in GFET biosensors in a comprehensive manner that contains the device design, working principle, surface functionalization, and proof-of-concept applications. It provides a comprehensive survey of GFET biosensors with regard to biomarker analysis at the single-device level and integrated prototypes that include wearable sensors, biomimetic systems, healthcare electronics, and diagnostic platforms. Moreover, there is discussion on the long-standing research efforts and outlook for the future development of GFET sensor systems from lab to fab.

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Section: Biomarker Analysismentioning

confidence: 99%

Section: Infectious Virusmentioning

confidence: 99%

Section: Proteinmentioning

confidence: 99%

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Graphene Transistors for In Vitro Detection of Health Biomarkers

Dai

Kong

Chen

et al. 2023

Adv Funct Materials

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“…Besides detecting RNA and DNA, 2D material FET biosensors can detect viral proteins. For instance, Palacio et al have employed graphene-based FET biosensors to detect the presence of the hepatitis C virus core protein [ 204 ]. By measuring the degree of specific binding between the virus DNA/RNA or protein and the biosensor surface, the presence of the virus in the sample can be determined.…”

Section: Iterative Strategies Of Fetmentioning

confidence: 99%

Review on two-dimensional material-based field-effect transistor biosensors: accomplishments, mechanisms, and perspectives

et al. 2023

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Field-effect transistor (FET) is regarded as the most promising candidate for the next-generation biosensor, benefiting from the advantages of label-free, easy operation, low cost, easy integration, and direct detection of biomarkers in liquid environments. With the burgeoning advances in nanotechnology and biotechnology, researchers are trying to improve the sensitivity of FET biosensors and broaden their application scenarios from multiple strategies. In order to enable researchers to understand and apply FET biosensors deeply, focusing on the multidisciplinary technical details, the iteration and evolution of FET biosensors are reviewed from exploring the sensing mechanism in detecting biomolecules (research direction 1), the response signal type (research direction 2), the sensing performance optimization (research direction 3), and the integration strategy (research direction 4). Aiming at each research direction, forward perspectives and dialectical evaluations are summarized to enlighten rewarding investigations.

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“…18 Recently, a similar platform has been demonstrated, especially suitable for high-performance bioelectronic devices. 31 Afterwards, we carry out an in situ surface polymerization of 2-hydroxyethyl acrylate (HEA) monomer that grows in a controlled and reproducible manner 32 from the OH group provided by the p-AP molecule, resulting in a robust and stable graphene/p-AP/PHEA hybrid functional material. Our method keeps the suitable properties of functionalized graphene, since no solvents or high temperatures that could lead to the material degradation are used enhancing, in contrast, the versatility of graphene.…”

Section: Introductionmentioning

confidence: 99%

Acrylates Polymerization on Covalent Plasma-Assisted Functionalized Graphene: A Route to Synthesize Hybrid Functional Materials

Muñoz,

León-Boigues,

López-Elvira

et al. 2023

ACS Appl. Mater. Interfaces

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The modification of the surface properties of graphene with polymers provides a method for expanding its scope into new applications as a hybrid material. Unfortunately, the chemical inertness of graphene hinders the covalent functionalization required to build them up. Developing new strategies to enhance the graphene chemical activity for efficient and stable functionalization, while preserving its electronic properties, is a major challenge. We here devise a covalent functionalization method that is clean, reproducible, scalable, and technologically relevant for the synthesis of a large-scale, substratesupported graphene−polymer hybrid material. In a first step, hydrogen-assisted plasma activation of p-aminophenol (p-AP) linker molecules produces their stable and covalent attachment to large-area graphene. Second, an in situ radical polymerization reaction of 2-hydroxyethyl acrylate (HEA) is carried out on the functionalized surface, leading to a graphene−polymer hybrid functional material. The functionalization with a hydrophilic and soft polymer modifies the hydrophobicity of graphene and might enhance its biocompatibility. We have characterized these hybrid materials by atomic force microscopy (AFM), X-Ray photoelectron spectroscopy (XPS) and Raman spectroscopy and studied their electrical response, confirming that the graphene/p-AP/PHEA architecture is anchored covalently by the sp 3 hybridization and controlled polymerization reaction on graphene, retaining its suitable electronic properties. Among all the possibilities, we assess the proof of concept of this graphene-based hybrid platform as a humidity sensor. An enhanced sensitivity is obtained in comparison with pristine graphene and related materials. This functional nanoarchitecture and the two-step strategy open up future potential applications in sensors, biomaterials, or biotechnology fields.

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Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor

Cited by 21 publications

References 38 publications

Graphene Transistors for In Vitro Detection of Health Biomarkers

Graphene Transistors for In Vitro Detection of Health Biomarkers

Review on two-dimensional material-based field-effect transistor biosensors: accomplishments, mechanisms, and perspectives

Acrylates Polymerization on Covalent Plasma-Assisted Functionalized Graphene: A Route to Synthesize Hybrid Functional Materials

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