Attraction of<i>Plecia nearctica</i>(Diptera: Bibionidae) to Floral Lures Containing Phenylacetaldehyde

Arthurs, Steven; Tofangsazi, Nastaran; Meagher, Robert L.; Cherry, Ron

doi:10.1653/024.095.0132

Cited by 8 publications

(5 citation statements)

References 11 publications

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“…A sensitive chemosensory system is essential for insects to accomplish many important physiological behaviors, such as the detection of food, predators, hosts, oviposition sites, and mates 1 , 2 . Many proteins have been found to be involved in odorant reception in the antennae, the major chemosensory organ.…”

Section: Introductionmentioning

confidence: 99%

Structure-Based Analysis of the Ligand-Binding Mechanism for DhelOBP21, a C-minus Odorant Binding Protein, from Dastarcus helophoroides (Fairmaire; Coleoptera: Bothrideridae)

Li¹,

Yu²,

Yi³

et al. 2015

Int. J. Biol. Sci.

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Odorant binding proteins (OBPs) transport hydrophobic odor molecules across the sensillar lymph to trigger a neuronal response. Herein, the Minus-C OBP (DhelOBP21) was characterized from Dastarcus helophoroides, the most important natural parasitic enemy insect that targets Monochamus alternatus. Homology modeling and molecular docking were conducted on the interaction between DhelOBP21 and 17 volatile molecules (including volatiles from pine bark, the larva of M. alternatus, and the faeces of the larva). The predicted three-dimensional structure showed only two disulfide bridges and a hydrophobic binding cavity with a short C-terminus. Ligand-binding experiments using N-phenylnaphthylamine (1-NPN) as a fluorescent probe showed that DhelOBP21 exhibited better binding affinities against those ligands with a molecular volume between 100 and 125 Å³ compared with ligands with a molecular volume between 160 and 185 Å³. Molecules that are too big or too small are not conducive for binding. We mutated the amino acid residues of the binding cavity to increase either hydrophobicity or hydrophilia. Ligand-binding experiments and cyber molecular docking assays indicated that hydrophobic interactions are more significant than hydrogen-bonding interactions. Although hydrogen-bond interactions could be predicted for some binding complexes, the hydrophobic interactions had more influence on binding following hydrophobic changes that affected the cavity. The orientation of ligands affects binding by influencing hydrophobic interactions. The binding process is controlled by multiple factors. This study provides a basis to explore the ligand-binding mechanisms of Minus-C OBP.

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Section: Introductionmentioning

confidence: 99%

Structure-Based Analysis of the Ligand-Binding Mechanism for DhelOBP21, a C-minus Odorant Binding Protein, from Dastarcus helophoroides (Fairmaire; Coleoptera: Bothrideridae)

Li¹,

Yu²,

Yi³

et al. 2015

Int. J. Biol. Sci.

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“…Hyacinthin is also an attractant of other insect groups, including e.g. the western honey bee Apis mellifera (Hymenoptera, Apidae) [51] or a species of march y Plecia nearctica (Diptera, Bibionidae) [52]. Although the plants of E. albensis produce hyacintin, we were unable to observe any moths visiting the owers, even those active at night.…”

Section: Discussionmentioning

confidence: 74%

Why Does an Obligate Autogamous Orchid Produce Insect Attractants in Nectar? – A Case Study on Epipactis Albensis (Orchidaceae)

Jakubska-Busse

Czeluśniak

Kobyłka

et al. 2021

Preprint

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Background The flowers of some species of orchids produce nectar as a reward for pollination, the process of transferring pollen from flower to flower. Epipactis albensis is an obligatory autogamous species, does not require the presence of insects for pollination, nevertheless, it has not lost the ability to produce nectar, the chemical composition of which we examined by gas chromatography-mass spectrometry (GC-MS) method for identification of potential insect attractants. Results During five years of field research, we did not observe any true pollinating insects visiting the flowers of this species, only accidental insects as ants and aphids. As a result of our studies we find that this self-pollinating orchid produces in nectar inter alia aliphatic saturated and unsaturated aldehydes such as i.e. nonanal (pelargonal) and 2-pentenal as well as aromatic ones (i.e. syringaldehyde, hyacinthin). The nectar is low in alkenes, which may explain the absence of pollinating insects. Moreover, vanillin and eugenol derivatives, well-known as important scent compounds were also identified, but the list of chemical compounds is much poorer compared with a closely related species, insect-pollinating E. helleborine. Conclusion Autogamy is a reproductive mechanism employed by many flowering plants, including the orchid genus Epipactis, as adaptation to grow in habitats where pollinating insects are rare observed due to the lack of nectar-producing plants they feed on. The production of numerous chemical attractants by self-pollinated E. albensis confirms the evolutionary secondary process, i.e. transition from ancestral insect-pollinating species to obligatory autogamous.

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“…Contrarily, the compound’s attractivity to dipterans has been revealed only in three Nematocera species, belonging to the Bibionidae ( Plecia nearctica Har. : [ 80 ]), Culicidae ( Culex pipiens Lin. : [ 81 ]) and Scatopsidae ( Coboldia fuscipes Meig.…”

Section: Discussionmentioning

confidence: 99%

Wide Range of Brachyceran Fly Taxa Attracted to Synthetic and Semi-Synthetic Generic Noctuid Lures and the Description of New Attractants for Sciomyzidae and Heleomyzidae Families

Nagy

Katona²,

Molnár

et al. 2023

Insects

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During field tests implemented in Transcarpathia (West Ukraine) in 2015, 6501 specimens belonging to 26 Brachyceran fly families were collected with traps baited with generic lures (originally developed for noctuid moths) based on fermenting liquid and floral compounds. Isoamyl alcohol-based baits generally attracted more flies than phenylacetaldehyde-based baits and unbaited controls; however, the phenylacetaldehyde-based traps were the most attractive to the Empididae and Milichiidae families. The isoamyl alcohol-based semisynthetic lure showed significant attractivity to the families of Muscidae, Ulidiidae, Sarcophagidae, Calliphoridae, Sciomyzidae, Heleomyzidae, Drosophilidae, Phoridae and Platystomatidae. Additionally, isoamyl alcohol-based semisynthetic lure is the first reported attractant of the Sciomyzidae family. Since our phenylacetaldehyde-based floral lure was also attractive to Heleomyzidae flies, both types of lures can be seen as the first known attractants of this family.

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Attraction ofPlecia nearctica(Diptera: Bibionidae) to Floral Lures Containing Phenylacetaldehyde

Cited by 8 publications

References 11 publications

Structure-Based Analysis of the Ligand-Binding Mechanism for DhelOBP21, a C-minus Odorant Binding Protein, from Dastarcus helophoroides (Fairmaire; Coleoptera: Bothrideridae)

Structure-Based Analysis of the Ligand-Binding Mechanism for DhelOBP21, a C-minus Odorant Binding Protein, from Dastarcus helophoroides (Fairmaire; Coleoptera: Bothrideridae)

Why Does an Obligate Autogamous Orchid Produce Insect Attractants in Nectar? – A Case Study on Epipactis Albensis (Orchidaceae)

Wide Range of Brachyceran Fly Taxa Attracted to Synthetic and Semi-Synthetic Generic Noctuid Lures and the Description of New Attractants for Sciomyzidae and Heleomyzidae Families

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