Atypical cutaneous lymphocytic infiltrate and a role for quantitative immunohistochemistry and gene rearrangement studies

Fucich, Lee F.; Freeman, Scott F.; Boh, Erin E.; McBurney, Elizabeth I.; Marrogi, Aizen J.

doi:10.1046/j.1365-4362.1999.00809.x

Cited by 14 publications

(8 citation statements)

References 24 publications

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“…This latter finding was in agreement with the known expansion of the normally occurring CD7 − /CD4 + T cell subtype in some benign inflammatory dermatoses (31,32). This latter finding was in agreement with the known expansion of the normally occurring CD7 − /CD4 + T cell subtype in some benign inflammatory dermatoses (31,32).…”

Section: Normal and Nonneoplastic Human Tissuessupporting

confidence: 91%

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Saati¹,

Alibaud²,

Lamant³

et al. 2001

APPL. IMMUNOHISTOCHEM.

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CBC.37 monoclonal antibody (mAb) was generated using balb/c mice immunized with CEM T cell line. It was selected because of its strong reactivity on T lymphocytes on paraffin tissue sections. The anti-CD7 specificity of CBC.37 mAb was assessed by immunohistochemistry, cross-blocking, and crossimmunoprecipitation experiments using CBC.37 and the anti-CD7 mAb DK24. CBC.37 mAb immunoprecipitated a 40-kDa protein. Cross-blocking and cross-immunoprecipitation experiments demonstrated that the two antibodies recognized the same molecule. Immunostaining of a large number of reactive lymph nodes and B and T cell lymphomas confirms that CBC.37 mAb was directed against T cells. As expected, on reactive lymph nodes the staining pattern was comparable to that of CD3. Among the 110 T cell lymphomas examined, all T lymphoblastic lymphomas were positive (15+/15; 100%). As a result of the frequent loss of CD7 antigen, only 25+/95 (26%) of peripheral T cell neoplasms were found to be positive for CBC.37. A marked reduction in the number of CBC.37positive T cells was observed in 7 of the 60 cases of benign inflammatory dermatoses studied (≈12%). CBC.37 was unreactive with all healthy and neoplastic non-lymphoid samples examined. Because the lack of CD7 expression in T cell lymphomas is of diagnostic value, CBC.37 mAb in association with other anti-T cell antibodies working on paraffin sections could be of particular value in asserting the diagnosis of T cell lymphomas in routine histopathology.

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Section: Normal and Nonneoplastic Human Tissuessupporting

confidence: 91%

Untitled

Saati¹,

Alibaud²,

Lamant³

et al. 2001

APPL. IMMUNOHISTOCHEM.

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“…DNA cytophotometry, 33-37 nuclear morphometry, 38-41 immunohistochemistry, 42-57 chromosomal studies, [58][59][60] and, more recently, molecular genetic analysis of T-cell clonality 8,[55][56][57][61][62][63][64][65][66][67][68][69][70][71][72][73][74][75][76][77][78] have generated a significant amount of data that suggest their utility not only in the early diagnosis of MF but also potentially in the clinical management of MF patients. How these data may be integrated with light microscopy to aid in the definitive diagnosis of CTCL has not been standardized.…”

Section: Early Diagnosis Of Mf: the Role Of Ancillary Techniquesmentioning

confidence: 99%

“…80 That the more sensitive molecular studies may be able to unmask this subset of ''benign'' dermatoses is supported by studies showing clonal populations, 81,82 aberrant immunophenotypes, 57 and/or MF-like T-cell cytokine production profiles 83 in the peripheral blood of some patients diagnosed as ''parapsoriasis en plaques.'' More recently developed techniques, such as single-cell PCR combined with laser-assisted microdissection, that exceed the current sensitivity of PCR-based methods (;1% clonal detection threshold) 76,77 need further evaluation regarding their specificity.…”

Section: Early Diagnosis Of Mf: the Role Of Ancillary Techniquesmentioning

confidence: 99%

Defining early mycosis fungoides

Pimpinelli¹,

Olsen

Santucci³

et al. 2005

Journal of the American Academy of Dermatology

475

378

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This editorial review summarizes the results of 5 meetings sponsored by the International Society for Cutaneous Lymphoma at which the clinicopathologic and ancillary features of early mycosis fungoides were critically examined. Based on this analysis, an algorithm was developed for the diagnosis of early mycosis fungoides involving a holistic integration of clinical, histopathologic, immunopathologic, and molecular biological characteristics. A novel aspect of this algorithm is that it relies on multiple types of criteria rather than just one, for example, histopathology. Before its finalization, the proposed diagnostic algorithm will require validation and possibly further refinement at multiple centers during the next several years. It is anticipated that a more standardized approach to the diagnosis of early mycosis fungoides will have a beneficial impact on the epidemiology, prognostication, treatment, and analysis of clinical trials pertaining to this most common type of cutaneous lymphoma.

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“…Another application germane to this fi eld was presented as Oral abstract 088 at 2nd world Congress of Cutaneous Lymphomas, Berlin 2013. Using the NUANCE imaging system, low-level CD30 expression was detected in CTCL and was proffered as an explanation for There has been scant literature in image cytometry application in cutaneous lymphoid infi ltrates with few exceptions, despite perceived advantages (Fucich et al 1999 ). The direct advantages include optimal use for enumerating the infi ltrative areas typical of skin biopsy, which are almost always limited in amount or almost always fi xed in formalin.…”

Section: Image Cytometrymentioning

confidence: 99%

Cutaneous Hematopathology

2014

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Atypical cutaneous lymphocytic infiltrate and a role for quantitative immunohistochemistry and gene rearrangement studies

Abstract: Quantitative immunophenotyping and gene rearrangement analysis can provide detailed information for classifying ACLIs with 91% diagnostic sensitivity and 87% specificity.

Cited by 14 publications

References 24 publications

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Defining early mycosis fungoides

Cutaneous Hematopathology

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