Atypical cutaneous mycobacteriosis diagnosed by polymerase chain reaction

Collina, Guido; Morandi, Luca; Lanzoni, Anna; Reggiani, Maria Letizia Bacchi

doi:10.1046/j.1365-2133.2002.04903.x

Cited by 31 publications

(18 citation statements)

References 12 publications

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Section: Discussionmentioning

confidence: 99%

“…A fish-to-human transmission of mycobacteria other than M. marinum is also possible. A recent report by Collina et al (2002) describes M. chelonae and M. fortuitum infection in 2 people who kept fish.Mycobacterium marinum infections in humans have been detected in Slovenia. Since 1999, 4 cases were reported by the University Clinic of Respiratory and Allergic Diseases Golnik (M. 5olnir-Dov< unpubl.…”

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Detection of mycobacteria in aquarium fish in Slovenia by culture and molecular methods

Pate

Jenčič²,

Žolnir-Dovč³

et al. 2005

Dis. Aquat. Org.

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Thirty-five aquarium fish were investigated for the presence of mycobacteria by culture and molecular methods. The following species were examined: goldfish Carassius auratus auratus, guppy Poecilia reticulata, 4 three-spot gourami Trichogaster trichopterus, dwarf gourami Colisa lalia, Siamese fighting fish Betta splendens, freshwater angelfish Pterophyllum scalare, African cichlid fish Cichlidae spp., cichlid fish Microgeophagus altispinosus, cichlid fish Pseudotropheus lombardoi, blue streak hap Labidochromis caeruleus, sterlet Acipenser ruthenus, southern platyfish Xiphophorus maculatus, and catfish Corydoras spp. Isolates of mycobacteria were obtained in 29 cases (82.9%). Two specimens were positive using Ziehl-Neelsen (ZN) staining, but the cultivation failed. Four specimens were both ZN-and culture-negative. On the basis of GenoType Mycobacterium assay (Hain Lifescience) and restriction enzyme analysis of the amplified products (PCR-RFLP), 23 isolates (79.3%) were identified: 7 as Mycobacterium fortuitum, 6 as M. gordonae, 6 as M. marinum, 3 as M. chelonae, and 1 as M. peregrinum. Five isolates remained unidentified (Mycobacterium spp.). One case probably represented a mixed infection (M. marinum/M. fortuitum). Since M. marinum infections are also detected in humans, the significance of mycobacteria in aquarium fish should not be overlooked. KEY WORDS: Mycobacteria · Aquarium fish · PCR · Restriction enzyme analysis Resale or republication not permitted without written consent of the publisherDis Aquat Org 64: [29][30][31][32][33][34][35] 2005 character of the disease, any suspicion of fish mycobacteriosis should be taken seriously and investigation of the case conducted.The diagnosis of mycobacterial disease in fish is based on histopathological, culture and molecular methods. The aim of the present study was to identify the species of mycobacteria isolated from aquarium fish in Slovenia in the period from 2001 to 2004 and to compare the available identification methods in order to assess their suitability for routine use. MATERIALS AND METHODSMaterials. The internal organs of 35 aquarium fish were investigated. The number of fish and the species investigated were: 11 goldfish Carassius auratus auratus, 7 freshwater angelfish Pterophyllum scalare, 4 three-spot gourami Trichogaster trichopterus, 3 guppy Poecilia reticulata, 2 southern platyfish Xiphophorus maculatus, 1 dwarf gourami Colisa lalia, 1 Siamese fighting fish Betta splendens, 1 African cichlid fish Cichlidae spp., 1 cichlid fish Microgeophagus altispinosus, 1 cichlid fish Pseudotropheus lombardoi, 1 blue streak hap Labidochromis caeruleus, 1 sterlet Acipenser ruthenus, and 1 catfish Corydoras spp.Bacteriology. Smears made directly from fish organs were stained with Ziehl-Neelsen (ZN). Following homogenization, decontamination with N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH), and concentration of the specimens, their sediments were inoculated on the following media: Stonebrink, Middlebrook 7H10, Löwenstein-Jensen with pyruvate, ...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Detection of mycobacteria in aquarium fish in Slovenia by culture and molecular methods

Pate

Jenčič²,

Žolnir-Dovč³

et al. 2005

Dis. Aquat. Org.

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“…Therefore, the best course is to cull and disinfect the premises. Rifampicin in combination with tetracycline (Boos et al, 1995) and clarithromycin may reduce infection (Collina et al, 2002).…”

Section: Wwwintechopencommentioning

confidence: 99%

Antibacterial Drugs in Fish Farms: Application and Its Effects

Sekkin¹,

Kum²

2011

Recent Advances in Fish Farms

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The world keeps changing. There are always risks associated with change. To make careful risk assessment it is always needed to re-evaluate the information according to new findings in research. Scientific knowledge is essential in determining the strategy for fish farming. This information should be updated and brought into line with the required conditions of the farm. Therefore, books are one of the indispensable tools for following the results in research and sources to draw information from. The chapters in this book include photos and figures based on scientific literature. Each section is labeled with references for readers to understand, figures, tables and text. Another advantage of the book is the "systematic writing" style of each chapter. There are several existing scientific volumes that focus specially on fish farms. The book consists of twelve distinct chapters. A wide variety of scientists, researchers and other will benefit from this book.

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“…The staining of histological specimens according to Ziehl-Neelsen (ZN) to detect mycobacteria is o�en negative as is the culture which should be performed under conditions allowing growth of atypical mycobacteria which usually require a lower in vitro culture temperature (about 25 to 30°C), through they can also grow when the temperatures reach about 37°C (Wayne and Kubica, 1986;Ang et al, 2000;Bha�y et al, 2000;Matlova et al, 2003). It is recommended to use biochemical examination or PCR detection for identification of the causative agent (Wayne and Kubica, 1986;Dvorska et al, 2001;Collina et al, 2002).…”

Section: Bacterial Pathogens Transmissiblementioning

confidence: 99%

Fish: a potential source of bacterial pathogens for human beings

Novotny¹,

Dvorská²,

Lorencova³

et al. 2004

Vet. Med.

208

187

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ABSTRACT:Human infections caused by pathogens transmi�ed from fish or the aquatic environment are quite common and depend on the season, patients' contact with fish and related environment, dietary habits and the immune system status of the exposed individual. They are o�en bacterial species facultatively pathogenic for both fish and human beings and may be isolated from fish without apparent symptoms of the disease. The infection source may be fish kept for both for food and as a hobby.

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Atypical cutaneous mycobacteriosis diagnosed by polymerase chain reaction

Cited by 31 publications

References 12 publications

Detection of mycobacteria in aquarium fish in Slovenia by culture and molecular methods

Detection of mycobacteria in aquarium fish in Slovenia by culture and molecular methods

Antibacterial Drugs in Fish Farms: Application and Its Effects

Fish: a potential source of bacterial pathogens for human beings

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