Atypical glandular cells (AGC): ThinPrep Imaging System (TIS), manual screening (MS), and correlation with Hybrid Capture 2 (HC2) HPV DNA testing

Sireci, Anthony; Crapanzano, John P.; Mansukhani, Mahesh; Babiac, B A Anamaria; Erroll, C T Maria; Vazquez, Madeline F.; Saqi, Anjali

doi:10.1002/dc.21273

Cited by 6 publications

(5 citation statements)

References 28 publications

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“…Fortunately, a number of studies, using various designs, found that the Imager algorithm does in fact identify and show to the screener abnormal glandular cells in most cases, resulting in no significant decrease in the detection of invasive or in situ adenocarcinoma when using this method. 3,[8][9][10] In one study, the sensitivity rate was found to be approximately similar to our study. TIS was able to identify atypical cells in 97% of these cases (68 of 70 cases) with a total number of 124 cases however they find 9 additional positive cases.…”

Section: Discussionsupporting

confidence: 88%

“…The widespread adoption of Imager led to some concern that the algorithm might not be optimal for the detection of glandular abnormalities. Fortunately, a number of studies, using various designs, found that the Imager algorithm does in fact identify and show to the screener abnormal glandular cells in most cases, resulting in no significant decrease in the detection of invasive or in situ adenocarcinoma when using this method 3,8–10 . In one study, the sensitivity rate was found to be approximately similar to our study.…”

Section: Discussionsupporting

confidence: 78%

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Diagnostic utility of ThinPrep Imaging System® for detecting atypical glandular cells in cervical smear samples

Akca

Erkılıç

2022

Diagnostic Cytopathology

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Introduction:The ThinPrep Imaging System ® (TIS) is an automated system that has now been used for over 20 years in the primary screening of ThinPrep liquid-based cervical samples. Although there are a lot of publications about the diagnostic utility of this method in squamous cell lesions, which has advantages such as time-saving and standardization, there are only a few publications on this issue in glandular cell lesions in the literature. We aimed in this study to investigate the diagnostic utility of the system in the detection of premalignant and malignant glandular lesions in cervical smears.Material and Method: Our study was conducted retrospectively, and a total of 126 cervical smear samples between 2010 and 2022 that have histological confirmation of endometrial adenocarcinoma (EAC), endocervical adenocarcinoma (ECAC), or adenocarcinoma in situ (A _ IS), were included. These samples were re-evaluated by manual and TIS by two experienced pathologists, and the results were compared in terms of sensitivity.Results: We found out that 70 of the 126 smear samples have atypical glandular cells. We detect 48 cases (48/70) (sensitivity 68.5%) in manual examination, however TIS successfully determined 66 cases (66/70) (sensitivity 94.3%). In 4 cases (5.7%) TIS could not detect the atypical cells within the 22 areas. Conclusion:TIS is quite an effective method with a high sensitivity for detecting atypical glandular cells in cervical smears, like detecting squamous cell anomalies.Imposing this system in our laboratory and using them appropriately, save us time and help to ensure standardization. Additionally, it may be a good way to adopt artificial intelligence and digital pathology in today's world.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 78%

Diagnostic utility of ThinPrep Imaging System® for detecting atypical glandular cells in cervical smear samples

Akca

Erkılıç

2022

Diagnostic Cytopathology

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“…Fortunately, a number of studies, using various designs, found that the Imager algorithm does in fact identify and show to the cytotechnologist abnormal glandular cells in most cases, resulting in no significant decrease in detection of invasive or in situ adenocarcinoma when using this method. [34][35][36] Likewise, detection of pathogenic organisms, though much less important, also appears not to decrease significantly when using the Imager for screening. 19,32 The Imager was the first commercial system to achieve significant commercial success.…”

Section: Thinprep Imaging System (Imager)mentioning

confidence: 99%

“…Since cases with no abnormalities in the 22 FOV selected by the Imager can be signed out as negative, many investigators feared that atypical glandular cells might be ignored by the algorithm and therefore would not be shown to cytotechnologists, resulting in false‐negative results. Fortunately, a number of studies, using various designs, found that the Imager algorithm does in fact identify and show to the cytotechnologist abnormal glandular cells in most cases, resulting in no significant decrease in detection of invasive or in situ adenocarcinoma when using this method . Likewise, detection of pathogenic organisms, though much less important, also appears not to decrease significantly when using the Imager for screening …”

Section: Papnetmentioning

confidence: 99%

Automated screening of Papanicolaou tests: A review of the literature

Thrall

2018

Diagnostic Cytopathology

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Automated Papanicolaou test screening systems have now been available for over 25 years. Currently two automated screening systems are in widespread clinical use. These are the ThinPrep Imaging System and the FocalPoint GS Imaging System. In their current configurations, both facilitate faster screening by showing a limited number of fields of view (FOV) to cytotechnologists. The FOV are based on the use of proprietary algorithms applied to computerized images of the slide that determine the cells and cell groups with the highest likelihood of abnormality. If all of the FOV are deemed to be negative, the case can be signed out with no additional review; if one or more fields appear possibly abnormal, the entire slide must be manually screened. The United States Food and Drug Administration has ruled that for workload calculation purposes, looking at only the FOV review counts as one-half slide, potentially greatly increasing the number of slides that can be screened. However, follow-up studies of this technology have shown that screening accuracy declines when very large numbers of cases are reviewed per day. Recommendations designed to limit screening volumes to levels that do not jeopardize patient care have therefore been created. The development of fully automated screening that does not rely on human judgment remains an unrealized aspiration. This review covers the history of the development and clinical implementation of automated screening technology with descriptions of the various automated screening systems and their performance as reported in published literature.

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“…The ThinPrep Papanicolaou-stained (Pap) smear has been one of the precancerous diagnosis methods of high-risk cervical carcinoma and has been widely used to screen the cervical cancer currently since it was developed in the 1990s [1] , [2] , [3] . ThinPrep liquid-based cytology has become a preferred method, of which samples were fixed in time, and blood, mucus and unstructured pieces were eliminated from the specimen.…”

Section: Introductionmentioning

confidence: 99%

Capture of cervical exfoliative cells on a glass slide coated by 3-glycidyloxypropyl trimethoxysilane and poly-L-lysine

Xing

Xiang

Xue

et al. 2012

Journal of Pharmaceutical Analysis

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A new modification method for glass slides was developed and applied to make ThinPrep Pap smears, in order to increase the adhesion ability of cervical exfoliative cells. 3-glycidyloxypropyl trimethoxysilane (GOPS) was coated on the glass slides firstly on the slides, then poly-L-lysine (PLL) was covalently modified onto the above epoxy-terminated slides to form GOPS-PLL double decorated slides. The modified slides were characterized using X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The cell adhesion ability effect was tested and compared with traditional PLL coated slides by fixing the cervical exfoliative cells on the double adorned slides. The control test was conducted by the bare glass slides unmodified. The cell morphology of cervical exfoliative cells adhered on different slides was observed under the microscope after Papanicolaou staining. The number of cervical exfoliative cells on the unmodified slides, PLL coated slides and GOPS-PLL coated slides was 1030±300, 3283±226 and 4119±280 ( n =12), respectively. The data among the three different modification methods showed significant differences (one-way analysis of variance, ANOVA test, P <0.05). The cell capturing effect of the GOPS-PLL slide was the best among the three different modified slides. In addition, the GOPS-PLL slide could enhance the uniformity of the adhered cells and be widely applied to the ThinPrep system for cervical carcinoma screening to increase the accuracy rate of diagnosis.

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Atypical glandular cells (AGC): ThinPrep Imaging System (TIS), manual screening (MS), and correlation with Hybrid Capture 2 (HC2) HPV DNA testing

Cited by 6 publications

References 28 publications

Diagnostic utility of ThinPrep Imaging System® for detecting atypical glandular cells in cervical smear samples

Diagnostic utility of ThinPrep Imaging System® for detecting atypical glandular cells in cervical smear samples

Automated screening of Papanicolaou tests: A review of the literature

Capture of cervical exfoliative cells on a glass slide coated by 3-glycidyloxypropyl trimethoxysilane and poly-L-lysine

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