Atypical<i>Salmonella enterica</i>serovars in murine and human infection models: Is it time to reassess our approach to the study of salmonellosis?

Hurley, Daniel; Hoffmann, Maria; Muruvanda, Tim; Allard, Marc W.; Brown, Eric W.; Martins, Marta; Fanning, Séamus

doi:10.1101/058610

Cited by 2 publications

(2 citation statements)

References 86 publications

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“…Several studies have reported that S. enterica serovar Typhimurium and other S. enterica serovars which grow as yellow colonies on XLD agar [72,73]. Out of the 195 colonies that grew on the presumptive XLD agar, only 35 were red with black centers while 160 colonies were yellow pigmented, and some with black centers.…”

Section: Discussionmentioning

confidence: 99%

Detection and Molecular Identification of Salmonella Virulence Genes in Livestock Production Systems in South Africa

2019

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Livestock are an important source of protein and food for humans, however opportunistic pathogens such as Salmonella spp. turn livestock into vehicles of foodborne diseases. This study investigated the prevalence of virulence genes in Salmonella spp. isolated from livestock production systems in two provinces of South Africa. During the period from May to August, 2018, a total of 361 faecal (189), oral (100), environmental (soil (36) and water (27)) and feed (9) samples were randomly collected from different animals (cattle, sheep, goats, pigs, ducks and chickens) that were housed in small-scale livestock production systems from Eastern Cape and KwaZulu-Natal Provinces in South Africa. Salmonella spp. were isolated and identified using microbiological and DNA molecular methods. Salmonella spp. were present in 29.0% of the samples of which 30.2% belonged to the Salmonella enterica species as confirmed by the positive amplification of the species specific iroB gene. Virulence genes that were screened from livestock-associated Salmonella were invA, iroB, spiC, pipD and int1. Statistically significant associations (p < 0.05) were established between the virulence genes, sampling location, animal host as well as the season when samples were collected. Furthermore, statistically significant (p < 0.05) positive correlations were observed between most of the virulence genes investigated. This is one of the recent studies to detect and investigate livestock-associated Salmonella spp. in South Africa. This study highlights the importance of continuous monitoring and surveillance for pathogenic salmonellae. It also demonstrated the detection and prevalence of virulent Salmonella spp. harbored by livestock in South Africa. This study demonstrated the potential risks of pathogenic Salmonella enterica to cause foodborne diseases and zoonotic infections from farm-to-fork continuum using the global one-health approach.

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Section: Discussionmentioning

confidence: 99%

Detection and Molecular Identification of Salmonella Virulence Genes in Livestock Production Systems in South Africa

2019

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“…These primers were designed from the highly conserved sequences across all strain-specific regions in their reference genomes like STY0307 genes for S. Typhi and an intergenic region between SSPA1723a and SSPA1724 for S. Paratyphi A. Many studies have examined thousands of Salmonella genomes that are accessible and have discovered SNPs that are unique to particular lineages [32,[34][35][36]. In several South Asian nations, the predominant strain of S. Typhi is genotype 4.3.1 (H58), which is highly resistant to 27 antibiotics [36].…”

Section: Discussionmentioning

confidence: 99%

Source tracking of extensively drug resistant Salmonella Typhi in food and raw vegetables using molecular approaches

Huda,

Sohail,

Mirani

et al. 2024

J Infect Dev Ctries

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Introduction: Extensively drug resistant (XDR) strains of the Salmonella lineages have been reported to spread from Africa to South Asia. XDR strains are resistant to fluoroquinolones, chloramphenicol, co-trimoxazole, and ampicillin, resulting in treatment failure. The objectives of this study included the investigation of transmission of S. Typhi lineages and the identification of the potentially contaminated sources of the XDR typhoid outbreak from different urban areas by using molecular techniques. Methodology: Environmental samples, including food samples, were collected from different towns and the susceptibility of each isolate to the antimicrobial agents was examined. Molecular identification of different Salmonella lineages including S. Typhi, S. Paratyphi A, H58, and XDR was carried out through multiplex PCR. Results and Conclusions: A total of 328 environmental samples including raw vegetables, water, and bakery items were collected. More than half of the tested samples (64%) found harboring Salmonella spp. The Salmonella was confirmed through PCR amplification of species-specific markers that showed the presence of S. Typhi (40%), S. Paratyphi A (8%), H58 (7%), and XDR S. Typhi (6%). Raw vegetables had the highest number of Salmonella spp., indicating consumption of raw vegetables as a possible source of salmonellosis. XDR status was also affirmed through phenotypic antimicrobial susceptibility testing.

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AtypicalSalmonella entericaserovars in murine and human infection models: Is it time to reassess our approach to the study of salmonellosis?

Cited by 2 publications

References 86 publications

Detection and Molecular Identification of Salmonella Virulence Genes in Livestock Production Systems in South Africa

Detection and Molecular Identification of Salmonella Virulence Genes in Livestock Production Systems in South Africa

Source tracking of extensively drug resistant Salmonella Typhi in food and raw vegetables using molecular approaches

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