Atypical strains of<i>Candida albicans</i>recovered from AIDS patients

Pujol, Claude; Renaud, François; Mallié, M.; Meeûs, Thierry De; Bastide, J.-M.

doi:10.1080/02681219780001001

Cited by 37 publications

(52 citation statements)

References 32 publications

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“…The patterns of some type II C. stellatoidea isolates were indistinguishable from those of some C. albicans isolates, demonstrating a lack of species specificity or low resolving power, if one accepts C. stellatoidea as an independent species (170). It is more likely that C. stellatoidea type II represents a subgroup of C. albicans, as has been suggested (172,290). rDNA and mitochondrial DNA probes, therefore, have not been generally used in broad epidemiological studies of the infectious fungi, and neither method has been validated for the different levels of genetic resolution.…”

Section: Restriction Fragment Length Polymorphisms With Hybridizationmentioning

confidence: 99%

The Ins and Outs of DNA Fingerprinting the Infectious Fungi

Soll

2000

Clinical Microbiology Reviews

283

317

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Section: Restriction Fragment Length Polymorphisms With Hybridizationmentioning

confidence: 99%

The Ins and Outs of DNA Fingerprinting the Infectious Fungi

Soll

2000

Clinical Microbiology Reviews

283

317

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“…Although the initial reports of atypical C. albicans isolates that proved to be C. dubliniensis (15,19,26) and many of the subsequent reports on this new species involved work done with isolates from HIV-positive patients (12,13,23,27), it is recognized that this species is not restricted to HIV-positive individuals (9,10,14,16,17). However, it has been generally accepted that C. dubliniensis preferentially colonizes HIV-positive individuals.…”

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Racial Distribution of Candida dubliniensis Colonization among South Africans

et al. 2003

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“…Some authors have proposed that clonal propagation with a remaining capacity of recombination, shape the population structure of C. albicans (Caugant & Sandven 1993, Gräser et al 1996, Tibayrenc 1997. Pujol et al (1997) demonstrated that the fingerprinting of C. albicans randon amplified polymorphic DNA (RAPD), multilocus enzyme electrophoresis (MLEE) and Southern blot hybridization with the moderately repetitive DNA Ca3 probe, not only clustered moderately related isolates in a similar fashion but also afforded similar levels of resolution of microevolution within a clonal population.…”

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confidence: 99%

First characterization of Candida albicans by random amplified polymorphic DNA method in Nicaragua and comparison of the diagnosis methods for vaginal candidiasis in Nicaraguan women

Bello

González

Barnabé

et al. 2002

Mem. Inst. Oswaldo Cruz

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The genital candidiasis is one of the pathogenic demonstrations of yeast. Candida albicans is the most frequent species; it is usually isolated in 85 to 90% from the vaginal mycoses (Odds et al. 1988).Vaginal candidiasis affects females at least once during their lifetime, at an estimated rate of 70 to 75%, of whom 40 to 50% will experience a recurrence (Sobel 1999).In Nicaragua, we know very little about the prevalence and incidence of vaginal candidiasis and no study of the biology of C. albicans has been carried out. In this country, diagnosis of vaginal candidiasis is mainly based on the clinical presentation. Laboratories of the hospitals and health centres (peripheral laboratories) carry out only the microscopic diagnosis from the vaginal fluid. In the laboratory of the National Centre of Diagnosis and Reference of Nicaragua (CNDR), the yeast identification is based on the observation of the microscopic aspects, culture and biochemical tests.Most of the genetic studies revealed that C. albicans is predominantly clonal (Pujol et al. 1993, Helstein et al. 1993, Lockhart et al. 1995, Xu et al. 1999. Some authors have proposed that clonal propagation with a remaining capacity of recombination, shape the population structure of C. albicans (Caugant & Sandven 1993, Gräser et al. 1996, Tibayrenc 1997 Southern blot hybridization with the moderately repetitive DNA Ca3 probe, not only clustered moderately related isolates in a similar fashion but also afforded similar levels of resolution of microevolution within a clonal population.The goal of this study was to use the RAPD method to examine the patterns of yeast genetic diversity among women with vaginitis from a single geographic area. We were specifically interested to know the frequency of yeast in vulvovaginal secretions. We also compared the conventional methods of yeast diagnosis from vaginal samples used in Nicaragua and yeast culture method. MATERIALS AND METHODSThe vaginal swabs were taken from 106 women exhibiting symptoms of vulvovaginitis, who were attended in the outpatient ward of the CNDR in Managua, Nicaragua, between June and August 1997. Swabs were processed by the method routinely used for the detection of germinated yeast pathogens: microscopic examination of wet mount, with a 10% potassium hydroxide (KOH) preparation, and the Gram's stain. Samples were inoculated into Sabouraud-glucose agar, supplemented with chloramphenicol, and were incubated at 37°C for 48 h. For identification of C. albicans, isolates were placed in foetal calf serum for 4 h to test for the production of germ tubes and were incubated on Rice-Agar-Tween (RAT ® ) BioMérieux Laboratories, France for 48 h to induce chlamydospores. All yeast isolates were preliminary identified to the species level according to the CHROMagar Albicans® Test (Mycoplasme International, Toulon, France). This medium contains a chromogene substrate for immediate identification of C. albicans (green), C. tropicalis (metallic blue), C. glabrata (pink) and C. krusei (pale pink). Yeast species were conf...

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Atypical strains ofCandida albicansrecovered from AIDS patients

Cited by 37 publications

References 32 publications

The Ins and Outs of DNA Fingerprinting the Infectious Fungi

The Ins and Outs of DNA Fingerprinting the Infectious Fungi

Racial Distribution of Candida dubliniensis Colonization among South Africans

First characterization of Candida albicans by random amplified polymorphic DNA method in Nicaragua and comparison of the diagnosis methods for vaginal candidiasis in Nicaraguan women

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