Au/In₂O₃ Nanocubes Based Label‐free Aptasensor for Ultrasensitive and Rapid Recognition of Cardiac Troponin I

Abstract

Cardiac Troponin I (cTnI) is a preferred biomarker to diagnose acute myocardial infarction which is one of the leading risks to health globally due to its short term. However, clinical analyzers are difficult to achieve its on‐site quantitative detection. A novel label‐free aptasensor was constructed to realize ultrasensitive and rapid recognition of cTnI. A nanocubic AuNPs/In2O3 composite was designed to provide synergistic effects of abundant active sites and signal magnification for aptamers grafting. Relying on a conductance‐dependence strategy, this aptasensor can achieve the quantitative detection within 10 min, which is much faster than state‐of‐the‐art analyzers, as well as exhibiting an ultrawide linear range of 0.1–1000 ng/mL and a low detection limit of 0.06 ng/mL with an excellent selectivity in the analysis of human serum.

Electrochemical Sandwich-Type Aptasensor Based on the Multifunctional Catechol-Loaded Au/MIL-53(Fe) for Detection of Cardiac Troponin I

“…The clinical diagnosis for AMI is conducted using electrocardiography (ECG), coronary angiography, and assessment of biomarker levels. In contrast to the low accuracy of ECG, with 57% of patients diagnosed correctly, and utilizing the contrast agent in coronary angiography, which made this method invasive and time-consuming, the cTnI blood biomarker has been established as an extremely specific and sensitive measurement for a precise assessment of the progression of AMI disease. − As a result, a sensitive and simple method for detecting cTnI is extremely valuable for AMI patients’ early detection and critical treatment. − Nowadays, the primary method for detecting cTnI is based on the antigen–antibody interaction as an immunosensor − or DNA binding as an aptasensor. − Despite their high sensitivity and selectivity, immunosensors have several limitations, including low stability at high temperatures, a long period of the immune reaction, high antibody production costs, and the difficulty of chemically modifying antibodies for biological detection. − Target-binding aptamers with resistance to harsh conditions, ease of chemical synthesis, and high specific affinity for fast-capturing cTnI have been developed as alternatives to antibodies. , By using the systematic evolution of ligands by exponential enrichment (SELEX) technique, Ban and colleagues developed extremely sensitive and selective single-stranded DNA aptamers against cTnI . Studies revealed that compared to the anti-cTnI antibody, Tro4 and Tro6 aptamers exhibited superior binding abilities to cTnI, and their dissociation constants were lower than those of the anti-cTnI antibody .…”

In Situ Construction of Oriented Pt‐PANI Needle‐Like Nanoarrays‐Based Label‐Free Aptasensor for Ultrafast and Ultrasensitive Recognition of Cardiac Troponin I

“…[ 11 ] Nowadays, its work for cTnI detection mainly adopted the principle of immune reaction as a immunosensor [ 12 ] or DNA binding as a aptasensor. [ 13 ] The former one depends on the specific combination of antibody/antigen with cTnI to establish the relation between electric signal and target concentration. Although it is of a high sensitivity and selectivity, the immunosensor often spends more than half an hour to finish the analysis owing to the long period of the immune reaction.…”

“…[11] Nowadays, its work for cTnI detection mainly adopted the principle of immune reaction as a immunosensor [12] or DNA binding as a aptasensor. [13] The former one depends on the specific combination of antibody/antigen with cTnI to establish the relation between electric signal and target Due to the short course and high mortality of acute myocardial infarction (AMI) which will cause irreversible myocardial necrosis to induce cardiac arrest, its early diagnosis within minutes is always a challenge in clinical emergency. In this work, an ultrafast and ultrasensitive aptasensor is designed to achieve quantitatively detection of cardiac troponin I (cTnI) which is proved as a highly specific and sensitive biomarker to AMI, and within only 4 min, the cTnI concentration can be accurately reported.…”

TiO2 Nanoparticles Co-Sensitized with Graphene Quantum Dots and Pyrocatechol Violet for Photoelectrochemical Detection of Cr(VI)

“…4,5 Due to the merits of this emerging analytical method, PEC sensors have been effectively used for the detection of heavy metal ions, 2,6 protein biomarkers, 7,8 DNA, 9,10 and cells. 11,12 Upon optical excitation in a PEC cell, charge carriers are generated and migrated to the electrolyte/photoelectrode interface to drive redox reactions, generating a photocurrent/photovoltage. 9,13,14 When this cell is used as an analytical sensor, the presence of an analyte changes the type and/or rate of reactions that occur at the electrode/electrolyte interface, resulting in a change in the magnitude of the photo-induced signal.…”