1999
DOI: 10.1099/00221287-145-12-3331
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AUD4, a new amplifiable element from Streptomyces lividans The GenBank accession number for the sequence reported in this paper is AF072709.

Abstract: After transformation of the Streptomyces lividans chloramphenicol-sensitive, arginine-auxotrophic mutant strain AJ100 with a derivative of plasmid SCP2, some of the regenerated protoplasts contained an 82 kb DNA sequence amplified to several hundred copies per chromosome. The corresponding nonamplified sequence, called AUD4, was isolated from a λ phage genomic library of S. lividans 1326. Two cytosine residues were the only directly repeated nucleotides at the ends of the element, indicating that AUD4 is a cla… Show more

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Cited by 7 publications
(2 citation statements)
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“…In one, similar to that for AUD4 of S. lividans (26), misaligned pairing and recombination between RsA and RsB on different chromosomes would generate a duplication of the AUD; recombination between RsC and RsD, resulting in excision of the intervening sequences, would generate the structure shown in Fig. 3B from which higher-order amplifications could be generated by unequal crossing over.…”
Section: Discussionmentioning
confidence: 94%
“…In one, similar to that for AUD4 of S. lividans (26), misaligned pairing and recombination between RsA and RsB on different chromosomes would generate a duplication of the AUD; recombination between RsC and RsD, resulting in excision of the intervening sequences, would generate the structure shown in Fig. 3B from which higher-order amplifications could be generated by unequal crossing over.…”
Section: Discussionmentioning
confidence: 94%
“…CYP105D5 and surrounding genes had the closest homology to the S. lividans AUD4 gene cluster (24). A S. griseus protein associated with the metabolism of a vast array of xenobiotics is also encoded by a homologue with high identity (16,25).…”
Section: Resultsmentioning
confidence: 99%