We conducted large-scale, replicated experiments to test the effects of two parallel power lines on area use, behaviour, and activity of semidomestic reindeer in enclosures. Yearling female reindeer were released into four 50 × 400 m enclosures; two treatment enclosures with power lines and two control enclosures. Reindeer from two herds, one from Kautokeino (domestic tame) and one from Vågå, (domestic wild) were tested separately and compared. Individual location within the enclosures was not affected by the power lines. Effects on restless behaviour were ambiguous, with slightly more restless behaviour in the treatment enclosures for the domestic tame reindeer, while the domestic wild reindeer maintained a stable level in the treatment enclosures, increasing with time in the control enclosures. Activity changes were slightly more common among animals within treatment enclosures for both herds, with no indication of habituation during the experiment. The domestic wild reindeer had more than three times the amount of restless behaviour than the domestic tame reindeer. Our study indicates that for reindeer in enclosures, the disturbance from a power line construction is negligible. This suggests that power lines are a minor disturbing factor compared to human handling when using fenced in areas like grazing gardens in reindeer husbandry.