Background: The accurate retrieval of cancer data in health information systems depends on the correct coding and classification of data. Thus, it is essential to provide continuous training for clinical coders and ensure the effectiveness of this training to achieve high-quality data. Objectives: The present study aimed at evaluating the effectiveness of the training workshop on ICD-10 cancer coding guidelines for clinical coders. Methods: The present study was performed to evaluate the effectiveness of coding guidelines training regarding the second chapter of ICD-10 (2016 ed.). Forty-five clinical coders have participated in the study. A researcher-made questionnaire was provided for guidelines data gathering, with the scoring system of Likert (0 to 5 points) at the reaction level, and by and pre-and post-test questionnaires at the learning level. The descriptive statistics were applied on the reaction level and the paired sample t-test was performed on the reaction level data. Results: The results of descriptive statistics revealed that the learners had 84.4% of the average satisfaction with the training courses The paired-samples test indicated that, at the learning level, a significant difference (P = 0.000) existed between the mean pre-and post-test scores. Conclusions: Based on Kirkpatrick’s model, the training program resulted in satisfaction and improved the clinical coders skills regarding cancer coding. It is recommended that health information managers hold effective training courses to enhance the coders’ knowledge and skills.