Augmentation of anti-tumour activity of cisplatin by pectin nano-conjugates in B-16 mouse model: pharmacokinetics and in-vivo biodistribution of radio-labelled, hydrophilic nano-conjugates

Verma, Anita; Chanchal, A.; Chutani, Krishna

doi:10.1504/ijnt.2012.049452

Cited by 15 publications

(7 citation statements)

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“…Studies were conducted under standardized procedures on inbred, male 6–8-week-old Balb/c (18–20 g) mice (Verma et al 2012 ). Destructive sampling method was adopted for acquisition of biological samples (blood and tissues) for analysis.…”

Section: Methodsmentioning

confidence: 99%

Pharmacokinetics and biodistribution of negatively charged pectin nanoparticles encapsulating paclitaxel

Verma

Kumar

2013

Cancer Nano

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Pectin, a naturally occurring biopolymer has been found to have increasing applications in the pharmaceutical and biotechnology industry. Sugars with their three-dimensional structures are important for many biological functions. We report preparation of negatively charged pectin nanoparticles encapsulating paclitaxel, a broad-spectrum anticancer drug for possible therapeutic applications. The mean diameter of the nanoparticles was ~300–350 nm with an encapsulation efficiency ~17 %. The TEM studies indicated that the particles were spherical in shape and their sizes are in unison with the DLS size spectra. The surface charge of pectin polymer was −5 mV and of nanoparticles were ~ −32 mV. The enhanced surface charge shows greater stability. The high electrophoretic mobility of ~3.5 to 1.5 μmcm/Vs confirmed the nano-size of particles. We compared the cytotoxic effect of paclitaxel (Pax) per se, pectin nanoparticles (PPN), and pectin chains on Hep G2, hepatic carcinoma cell line. Dose-dependent cytotoxicity was observed, wherein ~21.7 ± 3.2 % cytotoxicity was observed by PPN, but Pax per se showed ~55.6 ± 3.5 % cytotoxicity in a 72-h assay. The pharmacokinetics and biodistribution studies on Balb/c mice indicated that the nanoparticles had prolonged plasma retention of the drug with major accumulation in liver tissue after an i.v. tail vein injection of 20 mg/kg drug. The rank order of concentration are as follows, i.e., liver > kidney > lung > spleen for the PPN and spleen > liver > kidney ≥ lung for Pax per se. The in vitro studies clearly indicated that the efficacy of the drug was not compromised by encapsulation, making it a good candidate to deliver biopharmaceuticals. Nanoparticles produced free radicals in the free cell system and this ability caused oxidative stress, which may give rise to inflammation, cell destruction, and genotoxicity. Thus, the results obtained in this study holds great promise for pectin nanoparticles to be exploited for passive delivery of paclitaxel to tumor tissues, in particular, liver cancers.

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Section: Methodsmentioning

confidence: 99%

Pharmacokinetics and biodistribution of negatively charged pectin nanoparticles encapsulating paclitaxel

Verma

Kumar

2013

Cancer Nano

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“…Molecular pathways leading to targeted drug delivery and various diseases regulated with modified pectin. [108][109][110][111] Created with BioRender.com. [112] Psyllium moringa gum alginate Ciprofloxacin Hydrogel beads Anti ulcer potential "anti oxidative potential Slow and sustained drug delivery [113] Calcium alginate based sphere-in-capsule structure Bovine serum albumin Ranitidine hydrochloride…”

Section: Future Perspectivesmentioning

confidence: 99%

“… Molecular pathways leading to targeted drug delivery and various diseases regulated with modified pectin [108–111] . Created with BioRender.com.…”

Section: Significance Of Selected Polysaccharidesmentioning

confidence: 99%

Diversity of Rationally Modified Polysaccharides for Pharmaceutical Applications

et al. 2023

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The pressing need for the development of diversified and non‐toxic biomaterials in recent decades has led to the emergence of novel and valuable structural modifications in polysaccharides to broaden their spectrum of applications. Polysaccharides are macromolecules with multifaceted diverse structures and exhibit wide range of pharmacological and biological activities. Naturally occurring polysaccharides such as starch, chitosan, alginate, acetalated dextran and pectin could potentially negate major concerns associated with the selection of suitable polysaccharides without compromising their unique bioactive nature. Chemical modification of polysaccharides offers distinctive benefits such as improved biocompatibility, stability, solubility, and functionality making them more valuable for a variety of biomedical applications such as drug delivery, gene delivery, cell culture technology and tissue engineering. The present review is a compendium of relevant chemically modified polysaccharides and discusses their role in diverse fields of pharmacy and medicine.

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“…The anti-tumor efficacy was checked by measuring tumor growth. Cisplatin reduced the tumor size effectively, as studied by tumor regression studies [ 105 ]. Nano-conjugates of pectin and cisplatin were more effective when given along with radiotherapy than cisplatin.…”

Section: Polysaccharides Based Drug Carriersmentioning

confidence: 99%

Polysaccharide-Drug Conjugates: A Tool for Enhanced Cancer Therapy

et al. 2022

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Cancer is one of the most widespread deadly diseases, following cardiovascular disease, worldwide. Chemotherapy is widely used in combination with surgery, hormone and radiation therapy to treat various cancers. However, chemotherapeutic drugs can cause severe side effects due to non-specific targeting, poor bioavailability, low therapeutic indices, and high dose requirements. Several drug carriers successfully overcome these issues and deliver drugs to the desired sites, reducing the side effects. Among various drug delivery systems, polysaccharide-based carriers that target only the cancer cells have been developed to overcome the toxicity of chemotherapeutics. Polysaccharides are non-toxic, biodegradable, hydrophilic biopolymers that can be easily modified chemically to improve the bioavailability and stability for delivering therapeutics into cancer tissues. Different polysaccharides, such as chitosan, alginates, cyclodextrin, pullulan, hyaluronic acid, dextran, guar gum, pectin, and cellulose, have been used in anti-cancer drug delivery systems. This review highlights the recent progress made in polysaccharides-based drug carriers in anti-cancer therapy.

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Augmentation of anti-tumour activity of cisplatin by pectin nano-conjugates in B-16 mouse model: pharmacokinetics and in-vivo biodistribution of radio-labelled, hydrophilic nano-conjugates

Cited by 15 publications

References 0 publications

Pharmacokinetics and biodistribution of negatively charged pectin nanoparticles encapsulating paclitaxel

Pharmacokinetics and biodistribution of negatively charged pectin nanoparticles encapsulating paclitaxel

Diversity of Rationally Modified Polysaccharides for Pharmaceutical Applications

Polysaccharide-Drug Conjugates: A Tool for Enhanced Cancer Therapy

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