Augmentation of TH-1 type response by immunoactive AT oligonucleotide from lactic acid bacteria via Toll-like receptor 9 signaling

Shimosato, Takeshi; Kitazawa, Haruki; Katoh, Shinichiro; Tohno, Masanori; Iliev, Iliyan D.; Nagasawa, Chiho; Kimura, Toshiro; Kawai, Yasushi; Saito, Tadao

doi:10.1016/j.bbrc.2004.11.119

Cited by 61 publications

(63 citation statements)

References 30 publications

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“…TLR2 and TLR4 are expressed at the cell surface, while TLR9 is expressed in endosomes (34). Recently, the specific TLRs involved in LAB recognition and cytokine production by DCs and macrophages have been identified (35)(36)(37)(38). Rigaux et al have reported that IL-12p40 secretion by mouse DCs in response to L. plantarum NCIMB8826 was dependent on TLR2-and TLR9-mediated signaling (37).…”

Section: Discussionmentioning

confidence: 99%

“…Rigaux et al have reported that IL-12p40 secretion by mouse DCs in response to L. plantarum NCIMB8826 was dependent on TLR2-and TLR9-mediated signaling (37). A second report showed that a CpG oligonucleotide from some LAB activates the TLR9 signaling pathway (38). Endosomal TLR9 recognition of bacterial oligonucleotide may occur after phagocytosis and digestion of bacteria.…”

Section: Discussionmentioning

confidence: 99%

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A sulfated polysaccharide, fucoidan, enhances the immunomodulatory effects of lactic acid bacteria

Kawashima

Murakami²,

Nishimura³

et al. 2011

Int J Mol Med

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Abstract. Fucoidan, a sulfated polysaccharide contained in brown algae, has a variety of immunomodulatory effects, including antitumor and antiviral effects. On the other hand, lactic acid bacteria (LAB) also have immunomodulatory effects such as anti-allergic effects. In this study, we demonstrated that fucoidan enhances the probiotic effects of LAB on immune functions. By using Peyer's patch cells and spleen cells in vitro, fucoidan amplified interferon (IFN)-γ production in response to a strain of LAB, Tetragenococcus halophilus KK221, and this activity was abolished by desulfation of fucoidan. Moreover, this IFN-γ response was abolished by interleukin (IL)-12 neutralization. These results indicate that fucoidan enhanced IL-12 production in response to KK221, resulting in promoting IFN-γ production. In an in vivo study, Th1/Th2 immunobalance was most improved by oral administration of both fucoidan and KK221 to ovalbumin-immunized mice. These findings suggest that fucoidan can enhance a variety of beneficial effects of LAB on immune functions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A sulfated polysaccharide, fucoidan, enhances the immunomodulatory effects of lactic acid bacteria

Kawashima

Murakami²,

Nishimura³

et al. 2011

Int J Mol Med

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“…[19][20][21] It has also been shown that LAB contains CpG-like stimulatory oligonucleotides, which can directly ligate with TLR-2 and/or TLR-9. 22,23) Furthermore, DCs can directly present antigens from commensal bacteria to mesenteric lymph nodes and interact with T and B cells to maintain non-inflammatory immune responses. 24,25) Recent evidence indicates that DC is also required for the generation of Foxp3 + Tregs through a retinoic acid and TGF-β-dependent manner.…”

Section: Cd25mentioning

confidence: 99%

Lactobacillus acidophilus Strain L-92 Induces CD4+CD25+Foxp3+ Regulatory T Cells and Suppresses Allergic Contact Dermatitis

Shah

Saio

Yamashita

et al. 2012

Biological & Pharmaceutical Bulletin

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The anti-allergic mechanism of heat-killed Lactobacillus acidophilus strain L-92 has not been fully investigated. Recent studies have reported that CD4 CD25 Foxp3 (forkhead box P3) T regulatory (Treg) cells play important roles in controlling allergic diseases. Hence, we examined the effect of orally administered L-92 on CD4 CD25 Foxp3 cell populations. BALB/c mice were supplemented daily with L-92 by gavage for 5 weeks. 2,4-Dinitrofluorobenzene (DNFB) was used to induce allergic contact dermatitis (ACD) in mice. Fluorescent-activated cell sorter (FACS) analysis was used to determine CD4 CD25 Foxp3 T cell populations in spleen and cervical lymph nodes (CLN). Interleukin-10 (IL-10), transforming growth factor-β (TGF-β), and Foxp3 mRNA expressions in mouse ear skin were investigated by real-time reverse transcription-polymerase chain reaction (RT-PCR). The percentage of CD4 CD25 Foxp3 T cell populations were significantly increased in both spleen and CLN of L-92-fed group than vehicle and control. In addition, L-92 produced higher levels of Foxp3, IL-10 and TGF-β compared to control mice. These results suggest that L-92 can up-regulate the number of Treg cells to suppress the progression of DNFB-induced contact dermatitis in mice. Key words contact dermatitis; Lactobacillus acidophilus strain L-92; regulatory T cell; forkhead box P3Commensal bacteria of the gut flora affect host physiology through diverse mechanisms, including modulation of the host immune system and attenuation of gastrointestinal (GI) diseases. Some clinical and experimental animal studies reported that certain commensal bacteria may also be able to regulate immune responses outside of the GI tract. Among them, species of lactobacilli and bifidobacteria are prominent commensal bacteria with anti-allergic properties. For example, a double-blind placebo-control study, Lactobacillus (L.) rhamnosus GG 1) and L. fermentum VRI-003 PCC 2) reduced the development of eczema in young children. Within the experimental allergy animal models, several lactic acid bacterial (LAB) strains, including L. casei strain Shirota, 3) and L. brevis strain SBC8803 4) inhibits antigen specific immunoglobulin E (IgE) production, and L. paracasei strain KW3110 5) was found to suppress atopic dermatitis (AD)-like skin lesions. We also previously demonstrated that heat-killed L. acidophilus strain L-92 (L-92) can suppress both 2,4-dinitrofluorobenzene (DNFB) and mite antigen-induced AD-like skin lesions in mice.6) Despite some pre-clinical studies on animal models as well as clinical trials have highlighted the beneficial roles of these bacteria, the exact mechanisms behind the anti-allergic effects still remain obscure.Allergic contact dermatitis (ACD) is a delayed-type hypersensitivity caused by an allergic reaction to some chemical (hapten) in contact with the skin. The allergic condition causes itching and inflammation of the skin manifested by varying degrees of erythema, edema, and vesiculation. Knowledge of the mechanism of ACD was derived mainly from animal models in...

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“…shown that the bacterial cell wall (lipopolysaccharide, lipoteichoicacid, peptidoglycan) and metabolites (such as short chain fatty acid, secreted proteins and so on) of viable lactobacilli play a pivotal role in the maintenance of gastrointestinal immune homeostasis and prevention of inflammation 3,[5][6][7][8] The most common cause of gastroenteritis in people of all ages is enteric caliciviruses (SRSVs) and rotaviruses followed by other enteric viruses like hepatitis A and E virus and human cytomegalovirus (CMV). In developing countries, diarrhea caused by rotavirus in children is often a significant cause of infant morbidity and mortality 3) .…”

Section: Introductionmentioning

confidence: 99%

“…In developing countries, diarrhea caused by rotavirus in children is often a significant cause of infant morbidity and mortality 3) . Recently, several controlled clinical trials have shown that some strains of lactobacilli such as Lactobacillus casei strain GG 3,5,9-12) Lactobacillus reuteri 13) and Lactobacillus acidophilus 14) exhibit both therapeutic and prophylactic effects in children with viral but not bacterially-induced diarrhea [15][16] .…”

Section: Introductionmentioning

confidence: 99%

Inhibition of VSV by Extracellular RNA from Culture Filtrate of Lactobacillus DM8909 in Vitro

Ling

Tang

2011

J. Hard Tissue Biology.

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Extracellular nucleic acids were found in the culture filtrate of Lactobacillus DM8909 at exponential growth phase. The study focused on the source, characteristics, nucleotide sequence and biological functions of extracellular nucleic acids in Lactobacillus DM8909.Nucleic acids in the culture filtrate were proven to be RNA by agarose gel electrophoresis which closely paralleled bacterial growth. Different experimental procedures (according to viral infection course) to assay the potential antiviral activity of RNA were used. Cell survival and viral inhibition were determined by antiviral assay and confirmed by immunofluorescence. Results showed the "excess" extracellular RNA which could inhibit viral infection to the host cells in vitro.The "excess" extracellular RNA may be excreted from Lactobacillus DM8909. The possible mechanisms of antiviral activity include: 1) interfering virus attachment or entry into the cells, perhaps by steric hindrance; 2) conducting as a signaling molecule in the cross-talk between probiotics and host; 3) inducing the immunocytes to produce IFN-α to modulate virus infection. This is the first study demonstrating the excretion of RNA in gastrointestinal commensal bacteria. Extracellular RNA may function as a new signaling molecule in the cross-talk between host and commensal bacteria.

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Augmentation of TH-1 type response by immunoactive AT oligonucleotide from lactic acid bacteria via Toll-like receptor 9 signaling

Cited by 61 publications

References 30 publications

A sulfated polysaccharide, fucoidan, enhances the immunomodulatory effects of lactic acid bacteria

A sulfated polysaccharide, fucoidan, enhances the immunomodulatory effects of lactic acid bacteria

<i>Lactobacillus acidophilus</i> Strain L-92 Induces CD4<sup>+</sup>CD25<sup>+</sup>Foxp3<sup>+</sup> Regulatory T Cells and Suppresses Allergic Contact Dermatitis

Inhibition of VSV by Extracellular RNA from Culture Filtrate of Lactobacillus DM8909 in Vitro

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