Augmentation Potency of Ethanol Seed Extract of <i>Ricinodendron heudelotii</i> on Some Male Reproductive Hormones in Wistar Albino Rats

Abstract

The quest for the use of plants in the management of reproductive hormone dysfunction prompted this research. This study explored the effects of Ricinodendron heudelotti seed ethanol extract on some male reproductive hormones. Twenty five male albino rats were used. The animals were grouped into five and each group had five (5) albino rats. The extract was processed from the seeds of Ricinodendron heudelotti. Group A served as control, group B animals were administered with 300mg/kg.bw, while groups C, D and E animals were administered 450, 600 and 750 mg/kg.bw of the extract respectively for twenty one days. The animals were sacrificed and their blood samples were collected for the assay of testosterone, Follicle stimulating hormone (FSH) and Luteinizing hormone (LH). From the results obtained, the serum testosterone level of the experimental animals showed a concentration dependent increase in all the extract administered groups when compared with the value of the control group. FSH and LH levels showed an increase statistically (p≤0.05) in comparison to the level of the control. Ricinodendron heudelotti seed extract effectively increased the reproductive hormones of the animals by increasing the serum Testosterone, FSH and LH levels, hence suggests its effectiveness in enhancement of the male reproductive function.

Male Reproductive Hormonal Activity of Synergistic Ingestion of Aqueous Extract of Cyperus esculentus, Phoenix dactylifera and Cocos nucifera in Male Rat Model

“…Collection of blood samples: Sacrifice was done in three batches; the first was carried out after 7 days, the second after 14 days and the third after 21 days. Blood samples (5 mL) were collected through cardiac puncture from each rat and put in plain sample bottles for biochemical analysis of Prolactin, Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Testosterone using the methods as described by Odinga et al 30,31 . The testes were carefully harvested from each animal for histological examination.…”

Effects of Synergistic Consuption of Aqueous Extract of Cyperus esculentus, Phoenix dactylifera and Cocos nucifera on Hematological Indices in Male Rat Model

“…The mixture was filtered using a sieve and the resulting filtrate was put into a bottle until use. This synergistic mixture (STDC) was prepared daily for the 21 days of administration 35 .…”

“…C Group 1(control): Feed+water ad libitum C Group 2 STDC: 200 mg/kg of STDC C Group 3 STDC: 400 mg/kg of STDC The administration in both group 2 and 3 was done orally using an oral gavage tube. The administration lasted for 21 days and animals were sacrificed 35 .…”

“…Blood samples were collected through cardiac puncture and transferred into Ethylenediaminetetraacetic Acid (EDTA) sample bottles for hematological analysis. Blood for platelets count was collected in sodium citrate bottle and analyzed uncentrifuged 35 .…”

“…Haematological analysis: Hematological analysis was carried out as reported in method used by Odinga et al 35 . Parameters that were analyzed for hematological status include White Blood Cell (WBC), Hemoglobin (Hb), Red Blood Cell (RBC), Packed Cell Volume (PCV), White Blood Cell Count (WBC) and differentials (neutrophils, eosinophils, monocytes and lymphocytes) and platelets using an automated hematological machine; hematology analyzer (Abacus Junior Vet 5, Austria).…”