Augmented antibacterial activity of cefazolin with silver nanoparticles against Staphylococcus aureus and Escherichia coli

Batool, Arfa; Bore, Mercy Chepngetich; Wu, Jie; Li, Chaoyu; Zeng, Hongjuan

doi:10.1016/j.jddst.2023.104550

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…The bicinchoninic acid (BCA) assay was conducted to assess the amount of protein leakage after the disinfection treatment [18]. After undergoing UV irradiation, exposure to an electric field, and combined disinfection methods, the agar blocks were subjected to elution in 10 mL of PBS.…”

Section: Bicinchoninic Acid Assaymentioning

confidence: 99%

Enhancing Escherichia coli Inactivation: Synergistic Mechanism of Ultraviolet Light and High-Voltage Electric Field

Zhang,

Liang,

Pan

et al. 2024

Foods

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This study investigated the bactericidal effects of ultraviolet (UV) radiation, a high-voltage electric field (HVEF), and their combination on Escherichia coli. The results indicated that UV and combined disinfection were more effective with longer exposure, leading to significant reductions in microbial activity. Specifically, the single UV disinfection alone reduced activity by 3.3 log after 5 min, while combined disinfection achieved a 4.2 log reduction. In contrast, short-term HVEF treatment did not exhibit significant bactericidal effects, only achieving a reduction of 0.17 log in 5 min. Furthermore, prolonged exposure to both UV disinfection and an HVEF was found to damage cell membranes, ultimately causing cell death, while shorter durations did not. Despite rapid cell count decreases, flow cytometry did not detect apoptotic or necrotic cells, likely due to rapid cell rupture. This study suggests that combining UV radiation and an HVEF could be a promising approach for inhibiting bacterial reproduction, with HVEF enhancing UV effects. These findings provide insights for using combined HVEF and UV disinfection in food safety and preservation.

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Section: Bicinchoninic Acid Assaymentioning

confidence: 99%

Enhancing Escherichia coli Inactivation: Synergistic Mechanism of Ultraviolet Light and High-Voltage Electric Field

Zhang,

Liang,

Pan

et al. 2024

Foods

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“…The time-kill assay was performed in accordance with a previous report [23]. Commercial pasteurized milk, or SSS, was inoculated with five strains, each at a concentration of 10 6 CFU/mL, respectively.…”

Section: Time-kill Assays In Sss and Pasteurized Milkmentioning

confidence: 99%

Antibacterial Effect and Possible Mechanism of Sesamol against Foodborne Pathogens

Li,

Wu,

Yan

et al. 2024

Foods

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Food safety problems caused by foodborne pathogens have become a major public issue, and the search for efficient and safe bacteriostatic agents has gained attention. Sesamol (SE), a phenolic compound abundant in sesame oil, offers numerous health benefits and exhibits certain antibacterial properties. The purpose of this study was to evaluate the antibacterial effect and potential mechanisms of SE against representative foodborne pathogens, including Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Salmonella serovar Enteritidis. The results showed that SE significantly inhibited the growth of the five pathogenic bacteria in sterile saline and pasteurized milk by 2.16–4.16 log10 CFU/g within 48 h. The results of the minimum bactericidal concentration and time–kill assay showed that SE had a greater inhibitory effect on L. monocytogenes compared with other bacteria. Additionally, SE was found to alter the cell membranes’ permeability in these bacteria, resulting in the release of intercellular proteins and DNA. A scanning electron microscopy analysis showed that exposure to SE resulted in significant changes in bacterial morphology, producing cell shrinkage and deformation. These findings suggest that SE could inhibit both Gram-negative and Gram-positive bacteria by interfering with the function and morphology of bacterial cells.

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Sonochemical Synthesis of Silver Nanoparticles and its Nanomedicinal Activities Against Staphylococcus aureus

Reyes

2024

Orient. J. Chem

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The global rise of antibiotic-resistant bacteria due to the overuse of commercial antibiotics poses a significant public health threat. As a result, researchers have been exploring new antibacterial agents and approaches, including the field of nanomedicine. This study aims to produce silver nanoparticles (AgNPs) using lactose as a reducing agent through an ultrasound-assisted procedure and evaluate their anti-staphylococcal properties. AgNPs were synthesized using a one-pot ultrasound-assisted method with silver nitrate as the precursor and lactose as the reducing agent while the antibacterial activity was tested against Staphylococcus aureus using the agar well diffusion method. The synthesized AgNPs exhibited strong antibacterial activity, demonstrating potential as nanomedicine. These findings suggest that AgNPs could be valuable in combating antibiotic resistance and functional in the healthcare industry as an alternative antibacterial agent.

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Augmented antibacterial activity of cefazolin with silver nanoparticles against Staphylococcus aureus and Escherichia coli

Cited by 3 publications

References 29 publications

Enhancing Escherichia coli Inactivation: Synergistic Mechanism of Ultraviolet Light and High-Voltage Electric Field

Enhancing Escherichia coli Inactivation: Synergistic Mechanism of Ultraviolet Light and High-Voltage Electric Field

Antibacterial Effect and Possible Mechanism of Sesamol against Foodborne Pathogens

Sonochemical Synthesis of Silver Nanoparticles and its Nanomedicinal Activities Against Staphylococcus aureus

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