AuNP array coated substrate for sensitive and homogeneous SERS-immunoassay detection of human immunoglobulin G

Qu, Qi; Wang, Jing; Zeng, Chuan; Wang, Mengfan; Qi, Wei; He, Zhimin

doi:10.1039/d1ra02404c

Cited by 11 publications

(5 citation statements)

References 37 publications

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“…The high biocompatibility of gold and graphene is well known [ 95 , 96 ]. In addition, the uniform size and shape distribution of nanostructures can reduce signal variability; in particular, structural uniformity in surface-enhanced Raman scattering (SERS) can significantly amplify the analyte signal [ 97 , 98 , 99 , 100 , 101 ]. On the basis of these properties, Choi et al developed a graphene/gold-hybrid SERS aptamer sensor that can detect DA by directly culturing cells on the sensor surface [ 68 ].…”

Section: Metal/graphene-hybrid-based Aptamer Sensorsmentioning

confidence: 99%

Recent Advances in Aptamer-Based Sensors for Sensitive Detection of Neurotransmitters

2023

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In recent years, there has been an increased demand for highly sensitive and selective biosensors for neurotransmitters, owing to advancements in science and technology. Real-time sensing is crucial for effective prevention of neurological and cardiovascular diseases. In this review, we summarise the latest progress in aptamer-based biosensor technology, which offers the aforementioned advantages. Our focus is on various biomaterials utilised to ensure the optimal performance and high selectivity of aptamer-based biosensors. Overall, this review aims to further aptamer-based biosensor technology.

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Section: Metal/graphene-hybrid-based Aptamer Sensorsmentioning

confidence: 99%

Recent Advances in Aptamer-Based Sensors for Sensitive Detection of Neurotransmitters

2023

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“…Evaluation of the efficiency of the SERS substrate showed the nanomolar range of the epidermal growth factor receptor detection. In the work of Qu et al [ 109 ], the etched glass substrates were used with the layer of immobilized gold nanoparticles, followed by a protective polydopamine coating and antibody immobilization. The implementation of multicomposites based on several types of nanoparticles in combination with a Raman reporter molecule made it possible to obtain a multilayer structure.…”

Section: Application Of Sers and Sers-combined Raman Techniques In The Detection Of Biomoleculesmentioning

confidence: 99%

“…[ 99,109] Langmuir film fabrication Assembling of metal monolayer at an air-liquid interface, followed by the transfer of the film onto a solid support.…”

Section: Substrate Fabricationmentioning

confidence: 99%

Raman Scattering-Based Biosensing: New Prospects and Opportunities

et al. 2021

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The growing interest in the development of new platforms for the application of Raman spectroscopy techniques in biosensor technologies is driven by the potential of these techniques in identifying chemical compounds, as well as structural and functional features of biomolecules. The effect of Raman scattering is a result of inelastic light scattering processes, which lead to the emission of scattered light with a different frequency associated with molecular vibrations of the identified molecule. Spontaneous Raman scattering is usually weak, resulting in complexities with the separation of weak inelastically scattered light and intense Rayleigh scattering. These limitations have led to the development of various techniques for enhancing Raman scattering, including resonance Raman spectroscopy (RRS) and nonlinear Raman spectroscopy (coherent anti-Stokes Raman spectroscopy and stimulated Raman spectroscopy). Furthermore, the discovery of the phenomenon of enhanced Raman scattering near metallic nanostructures gave impetus to the development of the surface-enhanced Raman spectroscopy (SERS) as well as its combination with resonance Raman spectroscopy and nonlinear Raman spectroscopic techniques. The combination of nonlinear and resonant optical effects with metal substrates or nanoparticles can be used to increase speed, spatial resolution, and signal amplification in Raman spectroscopy, making these techniques promising for the analysis and characterization of biological samples. This review provides the main provisions of the listed Raman techniques and the advantages and limitations present when applied to life sciences research. The recent advances in SERS and SERS-combined techniques are summarized, such as SERRS, SE-CARS, and SE-SRS for bioimaging and the biosensing of molecules, which form the basis for potential future applications of these techniques in biosensor technology. In addition, an overview is given of the main tools for success in the development of biosensors based on Raman spectroscopy techniques, which can be achieved by choosing one or a combination of the following approaches: (i) fabrication of a reproducible SERS substrate, (ii) synthesis of the SERS nanotag, and (iii) implementation of new platforms for on-site testing.

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“…This work presents the development of a direct label-free SERS-based sensor for the detection of a model immunoglobulin G (IgG) based on stable and reproducible SERS substrate. IgG is the most common antibody in humans and its concentration in healthy adults varies from 7 to 18 mg/mL depending on the response against different pathogens, such as viruses and bacteria [ 21 ]. The fabrication and detection of various concentrations of IgG (1 to 150 μg/mL) were achieved by step-by-step functionalization of the AuNPs/PS 1250 -b-P2VP 1285 with anti-IgG and applying SERS spectroscopy and principal component analysis (PCA) analysis.…”

Section: Introductionmentioning

confidence: 99%

A Direct Immunoassay Based on Surface-Enhanced Spectroscopy Using AuNP/PS-b-P2VP Nanocomposites

Vieira

Bovolato

Fonseca

et al. 2023

Sensors

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A biosensor was developed for directly detecting human immunoglobulin G (IgG) and adenosine triphosphate (ATP) based on stable and reproducible gold nanoparticles/polystyrene-b-poly(2-vinylpyridine) (AuNP/PS-b-P2VP) nanocomposites. The substrates were functionalized with carboxylic acid groups for the covalent binding of anti-IgG and anti-ATP and the detection of IgG and ATP (1 to 150 μg/mL). SEM images of the nanocomposite show 17 ± 2 nm AuNP clusters adsorbed over a continuous porous PS-b-P2VP thin film. UV–VIS and SERS were used to characterize each step of the substrate functionalization and the specific interaction between anti-IgG and the targeted IgG analyte. The UV–VIS results show a redshift of the LSPR band as the AuNP surface was functionalized and SERS measurements showed consistent changes in the spectral features. Principal component analysis (PCA) was used to discriminate between samples before and after the affinity tests. Moreover, the designed biosensor proved to be sensitive to different concentrations of IgG with a limit-of-detection (LOD) down to 1 μg/mL. Moreover, the selectivity to IgG was confirmed using standard solutions of IgM as a control. Finally, ATP direct immunoassay (LOD = 1 μg/mL) has demonstrated that this nanocomposite platform can be used to detect different types of biomolecules after proper functionalization.

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AuNP array coated substrate for sensitive and homogeneous SERS-immunoassay detection of human immunoglobulin G

Abstract: An AuNP array coated substrate was developed for the SERS-immunoassay detection of human immunoglobulin G.

Cited by 11 publications

References 37 publications

Recent Advances in Aptamer-Based Sensors for Sensitive Detection of Neurotransmitters

Recent Advances in Aptamer-Based Sensors for Sensitive Detection of Neurotransmitters

Raman Scattering-Based Biosensing: New Prospects and Opportunities

A Direct Immunoassay Based on Surface-Enhanced Spectroscopy Using AuNP/PS-b-P2VP Nanocomposites

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