AuNP-DG: Deoxyglucose-Labeled Gold Nanoparticles as X-ray Computed Tomography Contrast Agents for Cancer Imaging

Aydogan, Bulent; Li, Ji; Rajh, Tijana; Chaudhary, Amitabh; Chmura, Steven J.; Pelizzari, Charles A.; Wietholt, Christian; Kurtoğlu, Metin; Redmond, Peter L.

doi:10.1007/s11307-010-0299-8

Cited by 76 publications

(74 citation statements)

References 24 publications

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“…In vitro cell labeling studies demonstrated that cells positive for the targeted receptor were able to internalize a greater mass of AuNPs compared with cells without the receptor or when treated with nontargeted AuNPs [47][48]61,[63][64][65][66][67][68][69][70][71][72]. AuNPs targeted to mineral surfaces were shown to reach a saturation point in the surface density of AuNPs labeling mineral surfaces in vitro [32,73], similar to the saturation point observed for the nontargeted delivery of AuNPs to cells.…”

Section: Deliverability and Targetingmentioning

confidence: 81%

“…Other molecules that have been investigated for improving colloidal stability include gum arabic [59,128], polyacrylic acid [63], polysaccharides [20,48] and polyvinyl alcohol [32,73,84,[129][130][131]. AuNPs have been surface functionalized with peptides [21,72], antibodies [23,47,[63][64][69][70], saccharides [20,48,[66][67], lipoproteins [22,132] and numerous small molecules [32,61,71,73,[122][123]125,[129][130][131] for active targeting. Molecular ligands can be strategically chosen for target-specific binding to a site of interest, including cancerous cells or tumors [21,33,[47][48]61,[63][64][65][66][67][68][69]…”

Section: Surface Functionalizationmentioning

confidence: 99%

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Gold Nanoparticles as Contrast Agents in x-ray Imaging and Computed Tomography

Cole

Ross

Tilley

et al. 2015

Nanomedicine (Lond.)

304

212

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Computed tomography enables 3D anatomic imaging at a high spatial resolution, but requires delivery of an x-ray contrast agent to distinguish tissues with similar or low x-ray attenuation. Gold nanoparticles (AuNPs) have gained recent attention as an x-ray contrast agent due to exhibiting a high x-ray attenuation, nontoxicity and facile synthesis and surface functionalization for colloidal stability and targeted delivery. Potential diagnostic applications include blood pool imaging, passive targeting and active targeting, where actively targeted AuNPs could enable molecular imaging by computed tomography. This article summarizes the current state of knowledge for AuNP x-ray contrast agents within a paradigm of key structure-property-function relationships in order to provide guidance for the design of AuNP contrast agents to meet the necessary functional requirements in a particular application. Functional requirements include delivery to the site of interest (e.g., blood, tumors or microcalcifications), nontoxicity during delivery and clearance, targeting or localization at the site of interest and contrast enhancement for the site of interest compared with surrounding tissues. Design is achieved by strategically controlling structural characteristics (composition, mass concentration, size, shape and surface functionalization) for optimized properties and functional performance. Examples from the literature are used to highlight current design trade-offs that exist between the different functional requirements.

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Section: Deliverability and Targetingmentioning

confidence: 81%

Section: Surface Functionalizationmentioning

confidence: 99%

Gold Nanoparticles as Contrast Agents in x-ray Imaging and Computed Tomography

Cole

Ross

Tilley

et al. 2015

Nanomedicine (Lond.)

304

212

View full text Add to dashboard Cite

show abstract

“…5 E-selectin peptides graed on liposome surface, 6 or 2-deoxy-D-glucose onto gold NPs 7 have also shown interesting accumulation in cancer cells due to their enhanced glucose consumption. Specic targeting of pancreatic ductal adenocarcinoma can be performed through plectin-1 targeted peptides (PTP) at the surface of hybrid iron oxide/polymeric NPs.…”

Section: Introductionmentioning

confidence: 99%

Functionalization of nano-emulsions with an amino-silica shell at the oil–water interface

et al. 2015

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ab Nano-emulsions are very promising nano-carriers with high potential for loading lipophilic drugs. However, the surface of oil nano-droplets is a dynamic oil/water interface stabilized by surfactants, and its chemical modification to graft ligands is highly challenging. In this study we developed a new protocol for modification of the nano-droplets surface through a silica shell terminated by amine functions. It enabled preparation of nanocapsules of 65, 85 and 120 nm diameters with a surface coverage of ca. 2 amino groups per nm 2 . The nanocapsule surface was then functionalized (41% efficiency) by a model fluorescent ligand (coumarin blue) with a carboxylic function. The evidence for the successful grafting was provided by spectrofluorometry, Förster resonance energy transfer, atomic force microscopy coupled with fluorescence imaging and fluorescence correlation spectroscopy. This simple protocol for surface functionalization of the liquid/liquid interface of lipid droplets may constitute a real advance regarding potential applications that need efficient decoration of droplets with ligands.

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“…Cells were allowed to incubate with targeted AuNP before being imaged. Glucose allowed for an increased uptake of AuNP to cells, resulting in a 4-fold increase in CT attenuation in these targeted cells over nontargeted trials [40]. Li et al similarly described attachment of 2-DG to AuNP and subsequent uptake into A-549 cells in vitro.…”

Section: Cancer Targeted Gold Nanoparticles Formulationsmentioning

confidence: 99%

Gold Nanoparticles as X-Ray, CT, and Multimodal Imaging Contrast Agents: Formulation, Targeting, and Methodology

Mahan

Doiron

2018

Journal of Nanomaterials

116

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Computed tomography (CT) is among the most popular medical imaging modalities due to its high resolution images, fast scan time, low cost, and compatibility with all patients. CT scans of soft tissues require the localization of imaging contrast agents (CA) to create contrast, revealing anatomic information. Gold nanoparticles (AuNP) have attracted interest recently for their use as CT CA due to their high X-ray attenuation, simple surface chemistry, and biocompatibility. Targeting molecules may be attached to the particles to allow for the targeting of specific cell types and disease states. AuNP can also be readily designed to incorporate other imaging contrast agents such as rare earth metals and dyes. This review summarizes the current state-of-the-art knowledge in the field of AuNP used as X-ray and multimodal contrast agents. Primary research is analyzed through the lens of structure-propertyfunction to best explain the design of a particle for a given application. Design specification of particles includes size, shape, surface functionalization, composition, circulation time, and component synergy. Key considerations include delivery of a CA payload to the site of interest, nontoxicity of particle components, and contrast enhancement compared to the surrounding tissue. Examples from literature are included to illustrate the strategies used to address design considerations.

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AuNP-DG: Deoxyglucose-Labeled Gold Nanoparticles as X-ray Computed Tomography Contrast Agents for Cancer Imaging

Abstract: Results from this study demonstrate enhanced uptake of 2-DG-labeled gold nanoparticle by cancer cells in vitro and warrant further experiments to study the exact molecular mechanism by which the AuNP-DG is internalized and retained in the tumor cells.

Cited by 76 publications

References 24 publications

Gold Nanoparticles as Contrast Agents in x-ray Imaging and Computed Tomography

Gold Nanoparticles as Contrast Agents in x-ray Imaging and Computed Tomography

Functionalization of nano-emulsions with an amino-silica shell at the oil–water interface

Gold Nanoparticles as X-Ray, CT, and Multimodal Imaging Contrast Agents: Formulation, Targeting, and Methodology

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