Aureimonas jatrophae sp. nov. and Aureimonas phyllosphaerae sp. nov., leaf-associated bacteria isolated from Jatropha curcas L.

Abstract

Aureimonas jatrophae sp. nov. and Aureimonas phyllosphaerae sp. nov., leaf-associated bacteria isolated from Jatropha curcas L. , originating from surface-sterilized leaf tissues of Jatropha curcas L. cultivars were characterized using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that all four isolates belong to the genus Aureimonas. In these analyses, strain L7-484 T appeared to be most closely related to Aureimonas ureilytica 5715S-12 T (95.7 % sequence identity). The 16S rRNA gene sequences of strains L7-456, L9-479 and L9-753 T were found to be identical and also shared the highest similarity with A. ureilytica 5715S-12 T (97.5 %). Both L7- 484T and L9-753 T contained Q-10 and Q-9 as predominant ubiquinones and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidyldimethylethanolamine, sulfoquinovosyldiacylglycerol and an aminophospholipid as the major polar lipids. C 18 : 1 v7c and C 16 : 0 were the major fatty acids. Similar to other species in the genus Aureimonas, hydroxylated fatty acids (e.g. C 18 : 1 2-OH) and cyclic fatty acids (C 19 : 0 cyclo v8c) were also present. The DNA G+C contents of L7-484 T and L9-753 T were 66.1 and 69.4 mol%, respectively. Strains L7-484 T and L9-753 T exhibited less than 40 % DNA-DNA hybridization both between themselves and to A. ureilytica KACC 11607

Aureimonas galii sp. nov. and Aureimonas pseudogalii sp. nov. isolated from the phyllosphere of Galium album

“…The genomic DNA G+C content determined for strains PP-WC-4G-234 T , PP-CE-2G-454 T , PP-WC-1G-202 and PP-CC-3G-650 was 66.4, 68.9, 67.4 and 70.5 mol%, respectively. This is in the range of the G+C content reported for Aureimonas species (63.9-71.8 %; Jurado et al, 2006;Weon et al, 2007;Kim et al, 2008;Rathsack et al, 2011;Madhaiyan et al, 2013). The genomic G+C content of strain PP-WC-4G-234…”

“…†Discrepancies found between the results of Madhaiyan et al (2013) and Cho et al (2015). ‡Discrepancies found between the results of Rathsack et al (2011) and Madhaiyan et al (2013). Fig.…”

“…In contrast to the new strains, Aureimonas phyllosphaerae DSM 25026 T , Aureimonas ureilytica . Data for growth temperature, NaCl and pH range tests given in parentheses were taken from the original species descriptions (Weon et al, 2007;Kim et al, 2008;Jurado et al, 2006;Madhaiyan et al, 2013;Cho et al, 2015). Good growth was obtained after 3 days of incubation between 15 and 28 C (no growth at 4 C or at 30 C or above; Fig.…”

“…At the time of writing, five further Aureimonas species have been described, namely Aureimonas ferruginea and Aureimonas rubiginis (Lin et al, 2013), Aureimonas jatrophae and Aureimonas phyllosphaerae (Madhaiyan et al, 2013), and Aureimonas glaciistagni (Cho et al, 2015). Members of the genus Aureimonas have been isolated from a subterranean cave , air (Weon et al, 2007), a water-cooling system (Kim et al, 2008), rusty iron plates (Lin et al, 2013), leaves of Jatropha curcas L. (Madhaiyan et al, 2013) and a melt pond on arctic sea ice (Cho et al, 2015).…”

Impact of <i>Azospirillum</i> sp. B510 Inoculation on Rice-Associated Bacterial Communities in a Paddy Field

“…Aurantimonas has been found in above-ground parts of plants such as rice, soybean, Arabidopsis thaliana , and Jatropha curcas (1, 5, 16, 18). Their population was likely controlled in a manner similar to that of legume-rhizobia symbiosis (10).…”