Aureobasidium iranianum, a new species on bamboo from Iran

Identification and Characterization of Leaf-Inhabiting Fungi from Castanea Plantations in China

“…Notes— Aureobasidium is a well-known genus comprising saprophytes, endophytes and pathogens on various substrates [ 28 , 29 , 30 , 31 , 32 ]. A. castaneae from Castanea henryi in this study is closely related to A. thailandense from unknown plants based on phylogenetic inference of ITS and LSU sequence data ( Figure 5 ).…”

Collophora hispanica, a New Pathogen and Potential Threat to the Almond Industry in Iran

“…) were used to amplify ITS1 + 5.8s gene + ITS2 region. The reaction mixture and thermal cycling condition were the same as Arzanlou and Khodaei ). PCR products were sequenced in both directions using the same PCR primers as for amplification and a bigdye terminator v. 3.1 cycle sequencing kit (Applied Biosystems) as recommended by vendor and analysed on a 3130xl Genetic Analyzer (Applied Biosystems ™ , Foster City, CA, USA).…”

Molecular characterisation and pathogenicity ofPhaeoacremoniumspp. associated with esca disease of grapevine in Northern Iran

“…For the strains identified as Phaeoacremonium, part of b-tubulin genes was amplified using primer sets T1 (O'Donnell and Cigelnik 1997) and Bt2b (Glass and Donaldson 1995). The reaction mixture and PCR conditions for the ITS region were the same as Arzanlou and Khodaei (2012) and Arzanlou and Bakhshi (2012). For the b-tubulin, PCR was carried out in a final volume of 12.5 ml containing 10-15 ng genomic DNA, 1.25 ml of 106 reaction buffer, 60 ml of 1 mM dNTPs, 1.5 MgCl 2 , 0.2 pM of each primer, 0.5 ml DSMO and 0.5 U Taq Polymerase.…”