Australian thraustochytrids: Potential production of dietary long-chain omega-3 oils using crude glycerol

Feeding Whole Thraustochytrid Biomass to Cultured Atlantic Salmon (Salmo salar) Fingerlings: Culture Performance and Fatty Acid Incorporation

“…TC 20 was maintained at the Australian National Algae Culture Collection, strain number CS-997, GenBank accession number JN675250. Strain isolation information, medium preparation and culturing conditions have been reported previously [3,24]. In brief, the growth medium consisted of 4% (w/v) pure and crude glycerol (with glycerol content of 16.4 g/L), 0.2% (w/v) peptone, 0.2% (w/v) yeast extract, 0.5% (w/v) monosodium glutamate, 0.2% (w/v) corn steep liquor, metal solution (1 mL/L) and vitamin solution (1 mL/L) [24].…”

“…Strain isolation information, medium preparation and culturing conditions have been reported previously [3,24]. In brief, the growth medium consisted of 4% (w/v) pure and crude glycerol (with glycerol content of 16.4 g/L), 0.2% (w/v) peptone, 0.2% (w/v) yeast extract, 0.5% (w/v) monosodium glutamate, 0.2% (w/v) corn steep liquor, metal solution (1 mL/L) and vitamin solution (1 mL/L) [24]. Cells were harvested after 7 days cultivation in shaking incubators at 25 • C, 150 rpm and pH 7 and centrifuged at 2500 rpm (500× g) at 20 • C for 10 min.…”

Algal biotechnology for pursuing omega-3 fatty acid (bioactive) production

“…Various inexpensive carbon sources, such as whey from the dairy industry, processed fruit pulp from the food processing industry (an important resource for recovering nutrients), bread and bakery waste, 20 crude glycerol, 14 oil cake, lignocellulose resource 21 and molasses can be tested for growing novel microalgal strains 22 . Such cost-effective carbon sources may lead to higher or comparative yield to glucose and glycerol, thus decreasing upstream processing costs.…”

“…An approximate three-fold improvement in carotenoid content in all isolates was achieved when glycerol was used as a carbon source in the production medium 12 . Based on fatty acid profiling, Australian thraustochytrids, in general, had a higher docosahexaenoic acid (DHA) content with DHA at 17-31% of total fatty acid 13,14 . We have evaluated the efficiency of various solvents, solvent combinations 15 and cell disruption methods for lipid extraction 16 to make the process cost-effective.…”

Microbial lipids as potential source to food supplements

“…Schizochytrium sp. are the main organisms investigated for DHA production in particular for the optimization of the culture condition [22,25,26,27,30,36]. Thanks to their A c c e p t e d M a n u s c r i p t efficiency to produce DHA, thraustochytrids participate actively in the transfer of this omega-3 into marine ecosystem and consequently to land.…”