“…Hence, an appropriate amount of industrial N fertilizer is needed to compensate for the annual soil N losses to maintain the high successive biomass yields of Miscanthus; otherwise, the significant decrease in soil N levels could be caused even over a short establishment period (Placek et al, 2018;Wang et al, 2017). However, many papers suggest that soil N contents are not affected much after Miscanthus establishment (Brami et al, 2020;Duan et al, 2019;Dufossé et al, 2014;Gregory et al, 2018;Kang et al, 2020;Krol et al, 2019;Xu et al, 2021) or even increased (Chen et al, 2009;Das et al, 2016;Dufossé et al, 2014;Kahle et al, 1999Kahle et al, , 2002Nebeská et al, 2018) to some degree in the absence of N supplement independent of the cultivation years and soil types. We previously found that the annual harvest of a large amount of biomass (around 22.22 t/ ha/year) over a long-term period (> 15 years) slightly increased total N (TN) contents of 0-100 cm soil in the absence of N fertilization (Zhao et al, 2020(Zhao et al, , 2021.…”