Authentication of Chinese Medicinal Materials by DNA Technology

Shaw, Pang‐Chui; Wang, J; But, P.P.H.

doi:10.1142/4700

Cited by 24 publications

(19 citation statements)

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“…For RAPD, the reproducibility is heavily affected by the quality and concentration of template DNA, the ratio of template DNA to primers, and slight fluctuations of reacting components or cycling parameters. In regards to the PCR-RFLP method, the length of PCR products also confined its utilization, as the number of restriction enzyme sites is limited in sequence segments between two primers (Wang et al 2000;Shaw et al 2002;Lian et al 2008). As diagnostic tools, the ITS sequences are especially useful for authenticating substitutes or adulterants which contain morphologically and/or phytochemically indistinguishable species (Joshi et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA

He¹,

Tian²,

Liu³

2010

Electro Journal of Biotech

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Abbreviations: AP-PCR: arbitraily primed PCRARMS: amplification-refractory mutation system DMSO: dimethyl sulfoxide GC: guanine-cytosine ITS: internal transcribed spacer MP: maximum parsimony nrDNA: nuclear ribosomal DNA RAPD: random amplified polymorphic DNA RFLP: restriction fragment length polymorphism SCAR: sequence characterized amplified region TCM: traditional Chinese medicineTraditionally, the authentication of the traditional Chinese medicines (TCM), Angelica sinensis, is based on slightly different morphological characters and complex compounds. Usually, those methods are simultaneously affected by several factors, leading to subtle and ambiguous results. In this study, the internal transcribed spacer (ITS) regions of A. sinensis and seven other Angelica species used as adulterants were sequenced. A pair of specific primers was designed from the polymorphic ITS regions to distinguish A. sinensis from the adulterants and regional substitutes. These ITS-derived primers amplified approximately 520 bp specific fragments from the adulterants, whereas no products was amplified with the DNA of A. sinensis. We tested eight commercially crude materials purchased in the market by using these specific primers. The result showed that there were four samples adulterating A.

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Section: Discussionmentioning

confidence: 99%

Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA

He¹,

Tian²,

Liu³

2010

Electro Journal of Biotech

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“…Arif and Saleem (2016) authenticated 6 different strains of S. fimicola by targeting full ITS region and also found 100% resemblance between strains isolated from two opposite slopes of Evolution Canyon, Israel. ITS region is highly conserved within species and sequence homology decreases between different species, or class (Shaw et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Isolation of Coprophilous Fungus Sordaria fimicola from Different Areas of Lahore-Pakistan, and Molecular Characterization by ITS and RAPD-SCAR PCR

Arif¹,

Saleem²

2017

IJAB

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Sixty-one isolates of Sordaria fimicola were isolated from the dung samples of cow (Bos taurus), horse (Equus caballus) buffalo (Bos taurus), donkey (Equus asinus), rabbit (Oryctolagus cuniculus), sheep (Ovis aries), camel (Camelus bactrianus) and goat (Capra hircus) collected from different areas of Lahore by adopting a moist chamber method. The frequency of occurrence (62.5%) was higher for horse samples followed by cow and donkey. Microscopic characters such as colour of mycelia mat, shape of perithecia, arrangements of ascospores in the ascus were observed for morphological identification of isolated fungus. ITS region 1 and RAPD-SCAR analyses were made for the molecular characterization of isolates of S. fimicola. Diversity indices showed that diversity exists in all isolates. A monomorphic band of 200bp was amplified by ITS1 and ITS2 universal primers in all the isolates and showed 100% resemblance with S. fimicola when introduced in the NCBI BLAST database. Four of eight random decamer RAPD primers after initial screening amplified 22 bands among eight isolates, of which 13(59.0%) were polymorphic bands with average 5.5 bands per primer. Cluster analysis showed that the similarity coefficients ranged from 0.2-0.6. RL-4 and RL-7 locus amplified by R3 primer were sent for direct sequencing and contained 485 and 811 bp nucleotides. Successful SCAR primers were developed from sequenced RAPD fragments and submitted to GenBank under the accession number KY098763 for RAPD locus RL-7 and KY120322 for RL-4. Two stable SCAR markers called SCAR400 and SCAR500 were developed successfully that can be used to differentiate isolates of different areas.

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“…Therefore, has been development several methods of species identification that allow to be applied to a large number of medicinal plants (Smillie and Khan, 2010). In the 1990 decade, the researchers began to work with the design of certification methods using the genome of medicinal plants (Shaw et al, 2002;Techen et al, 2004;Sucher and Carles, 2008;Hao et al, 2009a). These methods favored the DNA polymerase (PCR) technique and the use of different DNA thermostable polymerases.…”

Section: Conventional Methods For the Authentication Of Medicinal Plamentioning

confidence: 99%

DNA Barcoding: An Alternative for the Identification of the Medicinal Plants Employed in Mexico

Marcial-Quino¹,

Mendoza‐Espinoza²,

Palacios³

2015

J. of Plant Sciences

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The use of plants for the treatment of illness is a common practice in Mexico. Almost 80% of the population utilizes medicinal plants at some point of their lives. The high demand of these plants generates the production of a diversity of herbal products in some of which different vegetal species are used as adulterants that can generate non-desired effects. This problem has led to the search of different methods for the identification of the vegetal species used in the herbal preparations. However, the path has not been obstacle free, some of these methods requires specific characteristics of the analyzing material, others shows low reproducibility and high costs. In this context, the present review covers diverse aspects of the identification methods in particular, the DNA barcoding. This a simple, low cost methodology used to identify medicinal plant materials in various presentations. The implementation of this methodology for the quality control of medicinal plants products in Mexico, would bring a number of benefits both, the herbal industry and the consumers, since the authentication of medicinal plants by DNA barcoding will guarantee high quality herbal medicinal products, without adulterants and without side effects, which would have a direct impact on users health, in addition to detonate the growth of the national herbalist sector.

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Authentication of Chinese Medicinal Materials by DNA Technology

Cited by 24 publications

References 0 publications

Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA

Molecular authentication of the traditional Chinese medicinal plant Angelica sinensis based on internal transcribed spacer of nrDNA

Isolation of Coprophilous Fungus Sordaria fimicola from Different Areas of Lahore-Pakistan, and Molecular Characterization by ITS and RAPD-SCAR PCR

DNA Barcoding: An Alternative for the Identification of the Medicinal Plants Employed in Mexico

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