Authentication of commercial candy ingredients using <scp>DNA PCR</scp>‐cloning methodology

Muñoz-Colmenero, Marta; Martínez, José Luis; Roca, A.; García‐Vázquez, Eva

doi:10.1002/jsfa.7158

Cited by 11 publications

(15 citation statements)

References 26 publications

(37 reference statements)

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“…Despite legal regulations, undesired porcine DNA has been found in candies labeled as halal (Demirhan and others ), which represents clear fraud and also an ethical problem for halal consumers. Porcine DNA traces have also been detected in lacto‐ovo‐vegetarian candies (Muñoz‐Colmenero and others ), corroborating the occurrence of this type of adulteration and undermining vegetarian practices. Animal traces come from gelatin, which is commonly used as a gelling and thickening agent in several foodstuffs, as candies, drinks, water‐based desserts, and in the pharmaceutical industry to make pill wraps (Demirhan and others ).…”

Section: Introductionmentioning

confidence: 53%

“…The process of DNA extraction and analysis from candies has been described in detail in Muñoz‐Colmenero and others (). Briefly, the extraction of DNA was performed with DNeasy Mericon Food Kit (QUIAGEN), from 200 mg of candy.…”

Section: Methodsmentioning

confidence: 99%

“…The high variety of resources for the gelatin challenges the identification of species contained in products with gelatin. DNA has proven to be a useful tool for this purpose (Ardura and others ; Ardura and others ; Doosti and others ; Huang and others ; Karabasanavar and others ; Pegels and others ; Muñoz‐Colmenero and others ). In this pilot study carried out in Spain, we have analyzed gelatin‐based candies of different types (that is, marshmallows, gummies, and complex products), employing the polymerase reaction chain (PCR), cloning and sequencing of short species‐specific DNA fragments.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Different DNA Animal Species in Commercial Candy Products

Muñoz-Colmenero

Martínez

Roca

et al. 2016

Journal of Food Science

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Candy products are consumed all across the world, but there is not much information about their composition. In this study we have used a DNA-based approach for determining the animal species occurring in 40 commercial candies of different types. We extracted DNA and performed PCR amplification, cloning and sequencing for obtaining species-informative DNA sequences. Eight species were identified including fish (hake and anchovy) in 22% of the products analyzed. Bovine and porcine were the most abundant appearing in 27 samples each one. Most products contained a mixture of species. Marshmallows (7), jelly-types, and gummies (20) contained a significantly higher number of species than hard candies (9). We demonstrated the presence of DNA animal species in candy product which allow consumers to make choices and prevent allergic reaction.

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Section: Introductionmentioning

confidence: 53%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of Different DNA Animal Species in Commercial Candy Products

Muñoz-Colmenero

Martínez

Roca

et al. 2016

Journal of Food Science

Self Cite

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“…To detect mixed samples, cloning sequencing has been widely applied in candy, fish pulp, and fish feedstuff (Galal-Khallaf, Ardura, Borrell, & Garcia-Vazquez, 2016;Muñoz-Colmenero, Martínez, Roca, & Garcia-Vazquez, 2016). When this strategy was used for small berry fruit products, we obtained 10% sensitivity for fresh, pasteurized and HTHP small berry fruit juice.…”

Section: Discussionmentioning

confidence: 99%

Authentication of Small Berry Fruit in Fruit Products by DNA Barcoding Method

Yang

et al. 2018

Journal of Food Science

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Small berry fruit products are gaining an expanded market due to their high nutrition value. However, the authenticity of products is challenged by adulteration and mislabeling. To establish an accurate and robust method for identifying both known and unknown fruit species in small berry fruit products, DNA barcoding technology based on Sanger sequencing was adopted. To overcome the influence of processing conditions on DNA recovery, mini-barcodes of rbcL and ITS and a medium-barcode of psbA-trnH were applied. To identify ingredients in products containing mixed species, plasmid cloning was applied to separate mixed barcodes. The method established in this paper could detect 1% to 10% target species in mixed fruit juice.

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“…1 The advent of polymerase chain reaction (PCR) has attracted much attention from scientists. 2 PCR has been one of the most improtant techniques in modern molecular biology 3 and has widespread applications in many areas, for example, DNA cloning, 4 diagnosis therapy, 5 funcitonal analysis of genes. 6 The amplication technique can increase the number of copies of target genes by a magnification of several millions within hours, which allows detection and analysis of DNA sequences.…”

Section: Introductionmentioning

confidence: 99%

Effect of antifouling dendrimers and Au DENPs on the enhancement of PCR amplification

Zhang

Peng

et al. 2017

Can. J. Chem.

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Abstract:The polymerase chain reaction (PCR) has been considered as one of the most fundamental techniques to amplify and analyze specific DNA fragments in the field of molecular biology and clinical medicine. Recently, a variety of nanoparticles (NPs) have been regarded as a novel method to enhance quality and yield of PCR technique. Herein, we report the use of generation 5 (G5) poly(amidoamine) (PAMAM) dendrimers and dendrimer-entrapped gold nanoparticles (Au DENPs) modified with polyethylene glycol (PEG) moieties and/or acetyl groups as a novel class of enhancers to improve the PCR amplification. We set up the nonspecific PCR and two-round PCR as model systems to investigate mechanisms of enhanced PCR. Our results show that dendrimer-based derivatives seem to enhance the PCR specificity. It is worth noting that the modification of antifouling PEG significantly lowered the optimization capability of the corresponding dendrimers, while this inhibition effect can be remarkably compromised by the entrapment of Au NPs.Furthermore, we found that in the presence of Au NPs, the Au NPs-induced thermal conductivity could play the dominant role in the PCR optimization, while in the absence of Au NPs, electrostatic interaction of the dendrimers with PCR components may be the major factor affecting the PCR system. Our results also showed that the optimal concentrations of the materials in the two test systems were very close, which indicates that the developed dendrimer derivatives with good thermal stability may be the efficient PCR additives for enhancing different PCR systems.

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Authentication of commercial candy ingredients using DNA PCR‐cloning methodology

Abstract: 1BACKGROUND: Commercial candies are consumed by all population age sectors

Cited by 11 publications

References 26 publications

Detection of Different DNA Animal Species in Commercial Candy Products

Detection of Different DNA Animal Species in Commercial Candy Products

Authentication of Small Berry Fruit in Fruit Products by DNA Barcoding Method

Effect of antifouling dendrimers and Au DENPs on the enhancement of PCR amplification

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