Author Correction: CRISPR-Cas12a-assisted nucleic acid detection

Li, Shi‐Yuan; Cheng, Qiuxiang; Wang, Jing-Man; Li, Xiaoyan; Zhang, Zilong; Gao, Song; Cao, Ruibing; Zhao, Guoping; Wang, Jin

doi:10.1038/s41421-019-0083-0

Cited by 6 publications

(3 citation statements)

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“…CRISPR assay has become a very popular technology and has been deployed in a huge range of applications. The CRISPER/Cas12a assay is gradually being applied for the detection of pathogens ( Chen et al., 2018 ; Li et al., 2018 ). In future, maybe a simple, rapid, sensitive, and unaided eye visualization CRISPR-Cas12a detection system will be developed for molecular identification of P. cinnamomi without requiring technical expertise or ancillary equipment.…”

Section: Discussionmentioning

confidence: 99%

Rapid detection of Phytophthora cinnamomi based on a new target gene Pcinn13739

Chen

Jiao

Zhou

et al. 2022

Front. Cell. Infect. Microbiol.

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Phytophthora cinnamomi causes crown and root wilting in more than 5,000 plant species and represents a significant threat to the health of natural ecosystems and horticultural crops. The early and accurate detection of P. cinnamomi is a fundamental step in disease prevention and appropriate management. In this study, based on public genomic sequence data and bioinformatic analysis of several Phytophthora, Phytopythium, and Pythium species, we have identified a new target gene, Pcinn13739; this allowed us to establish a recombinase polymerase amplification–lateral flow dipstick (RPA-LFD) assay for the detection of P. cinnamomi. Pcinn13739-RPA-LFD assay was highly specific to P. cinnamomi. Test results for 12 isolates of P. cinnamomi were positive, but negative for 50 isolates of 25 kinds of Phytophthora species, 13 isolates of 10 kinds of Phytopythium and Pythium species, 32 isolates of 26 kinds of fungi species, and 11 isolates of two kinds of Bursaphelenchus species. By detecting as little as 10 pg.µl−1 of genomic DNA from P. cinnamomi in a 50-µl reaction, the RPA-LFD assay was 100 times more sensitive than conventional PCR assays. By using RPA-LFD assay, P. cinnamomi was also detected on artificially inoculated fruit from Malus pumila, the leaves of Rhododendron pulchrum, the roots of sterile Lupinus polyphyllus, and the artificially inoculated soil. Results in this study indicated that this sensitive, specific, and rapid RPA-LFD assay has potentially significant applications to diagnosing P. cinnamomi, especially under time- and resource-limited conditions.

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Section: Discussionmentioning

confidence: 99%

Rapid detection of Phytophthora cinnamomi based on a new target gene Pcinn13739

Chen

Jiao

Zhou

et al. 2022

Front. Cell. Infect. Microbiol.

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“…19,20 Most CRISPR/Cas12a-based nucleic acid detection systems are based on the cleavage of a fluorescence-active probe that contains a quencher and a fluorophore bridged by ssDNA, and a fluorescence “turn-on” will be observed when ssDNA is acted upon by Cas12/crRNA/target DNA. 21–23 Zhou et al developed a CRISPR/Cas12a-based magnetic pull-down-assisted colorimetric method (M-CDC) for the detection of SARS-CoV-2 with a detection limit of 50 RNA copies per reaction. 24 Taghdisi et al developed a colorimetrically sensitive sensor for the precise detection of aflatoxin M1 (AFM1) based on the peroxidase mimetic activity of AuNPs.…”

Section: Introductionmentioning

confidence: 99%

Sensitive detection of genetically modified maize based on a CRISPR/Cas12a system

Wang,

Su,

Chang

et al. 2024

Analyst

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“…22 By combining the high recognition ability of CRISPR/Cas with an optical sensing technology that amplifies the signal of the target gene, CRISPR/Cas system-based optical sensors can be constructed to achieve highly specific and sensitive detection of analytes. DETECTR (DNA endonuclease targeted CRISPR trans reporter), 23 HOLMES (one-hour low-cost multipurpose highly efficient system), 24,25 and SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) 26 are good examples, and they all use fluorescence signals to verify cleavage activity. In this review, we explore the use of CRISPR/Cas technology in combination with optical methods, including colorimetric, fluorescence, electrochemiluminescence, and surface-enhanced Raman scattering techniques, in various related fields.…”

Section: Introductionmentioning

confidence: 99%