Author Correction: Molecular architecture of lineage allocation and tissue organization in early mouse embryo

Peng, Guangdun; Suo, Shengbao; Cui, Guizhong; Yu, Fang; Wang, Ran; Chen, Jun; Chen, Shirui; Liu, Zhiwen; Chen, Guoyu; Qian, Yun; Tam, Patrick; Han, Jing‐Dong J.; Jing, Naihe

doi:10.1038/s41586-020-2755-1

Cited by 5 publications

(1 citation statement)

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“…Because TFs recognize DNA motifs in the genome, one can measure if inferred target genes are expressed within single cells, and therefore quantify the activity of TFs. Such approaches have revealed the regulatory programs in distinct systems including the Drosophila brain ( 76 ), cancer ( 77 ), during early mouse embryonic ( 78 ) and pancreas development ( 79 ), reprogramming to induced pluripotency ( 80 ), in a mouse cell atlas ( 81 ) and a human cell atlas ( 3 ).…”

Section: Introductionmentioning

confidence: 99%

Inferring regulators of cell identity in the human adult pancreas

Vanheer,

Fantuzzi,

et al. 2023

NAR Genomics and Bioinformatics

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Cellular identity during development is under the control of transcription factors that form gene regulatory networks. However, the transcription factors and gene regulatory networks underlying cellular identity in the human adult pancreas remain largely unexplored. Here, we integrate multiple single-cell RNA-sequencing datasets of the human adult pancreas, totaling 7393 cells, and comprehensively reconstruct gene regulatory networks. We show that a network of 142 transcription factors forms distinct regulatory modules that characterize pancreatic cell types. We present evidence that our approach identifies regulators of cell identity and cell states in the human adult pancreas. We predict that HEYL, BHLHE41 and JUND are active in acinar, beta and alpha cells, respectively, and show that these proteins are present in the human adult pancreas as well as in human induced pluripotent stem cell (hiPSC)-derived islet cells. Using single-cell transcriptomics, we found that JUND represses beta cell genes in hiPSC-alpha cells. BHLHE41 depletion induced apoptosis in primary pancreatic islets. The comprehensive gene regulatory network atlas can be explored interactively online. We anticipate our analysis to be the starting point for a more sophisticated dissection of how transcription factors regulate cell identity and cell states in the human adult pancreas.

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