Author response: Distinct myocardial lineages break atrial symmetry during cardiogenesis in zebrafish

Guerra, Almary; Germano, Raoul Fv; Stone, Oliver A.; Arnaout, Rima; Guenther, Stefan; Ahuja, Suchit; Uribe, Verónica; Vanhollebeke, Benoît; Stainier, Didier Y.R.; Reischauer, Sven

doi:10.7554/elife.32833.044

Cited by 3 publications

(1 citation statement)

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“…Vcl immunostaining showed the same localization pattern (Figure S3C). To examine Vcl-EGFP localization in more detail, we used the Tg(myl7:BFP-CAAX) line (Guerra et al, 2018) to label CM membranes with blue fluorescent protein (BFP). We found that Vcl-EGFP localized more specifically to cell-cell junctions in CMs at 127 hpf than 72 hpf (Figures 3A and 3B), as assessed by measuring Vcl-EGFP fluorescence intensity across the entire CM (Figures 3A and 3B).…”

Section: Cardiac Contractility Regulates Vcl Localization and Activationmentioning

confidence: 99%

Mechanical Forces Regulate Cardiomyocyte Myofilament Maturation via the VCL-SSH1-CFL Axis

et al. 2019

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Section: Cardiac Contractility Regulates Vcl Localization and Activationmentioning

confidence: 99%

Mechanical Forces Regulate Cardiomyocyte Myofilament Maturation via the VCL-SSH1-CFL Axis

et al. 2019

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Of form and function: Early cardiac morphogenesis across classical and emerging model systems

Shewale

Dubois

2021

Seminars in Cell & Developmental Biology

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The developing epicardium regulates cardiac chamber morphogenesis by promoting cardiomyocyte growth

Boezio

Gollin

Zhao

et al. 2021

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The epicardium, the outermost layer of the heart, is an important regulator of cardiac regeneration. However, a detailed understanding of the crosstalk between the epicardium and myocardium during development requires further investigation. Here, we generated three models of epicardial impairment in zebrafish by mutating the transcription factor genes tcf21 and wt1a, and by ablating tcf21+ epicardial cells. Notably, all three epicardial-impairment models exhibit smaller ventricles. We identified the initial cause of this phenotype as defective cardiomyocyte growth, resulting in reduced cell surface and volume. This failure of cardiomyocytes to grow is followed by decreased proliferation and increased abluminal extrusion. By temporally manipulating its ablation, we show that the epicardium is required to support ventricular growth during early cardiac morphogenesis. By transcriptomic profiling of sorted epicardial cells, we identified reduced expression of FGF and VEGF ligand genes in tcf21-/- hearts, and pharmacological inhibition of these signaling pathways partially recapitulated the ventricular growth defects. Thus, the analysis of these epicardial-impairment models further elucidates the distinct roles of the epicardium during cardiac morphogenesis and the signaling pathways underlying epicardial-myocardial crosstalk.

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Author response: Distinct myocardial lineages break atrial symmetry during cardiogenesis in zebrafish

Cited by 3 publications

References 0 publications

Mechanical Forces Regulate Cardiomyocyte Myofilament Maturation via the VCL-SSH1-CFL Axis

Mechanical Forces Regulate Cardiomyocyte Myofilament Maturation via the VCL-SSH1-CFL Axis

Of form and function: Early cardiac morphogenesis across classical and emerging model systems

The developing epicardium regulates cardiac chamber morphogenesis by promoting cardiomyocyte growth

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