Author response: Doa10 is a membrane protein retrotranslocase in ER-associated protein degradation

Schmidt, Claudia; Vasic, Vedran; Stein, Alexander

doi:10.7554/elife.56945.sa2

2020

DOI: 10.7554/elife.56945.sa2

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Author response: Doa10 is a membrane protein retrotranslocase in ER-associated protein degradation

Claudia Schmidt

Vedran Vasic

Alexander Stein

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2023

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Characterization of endoplasmic reticulum-associated degradation in the human fungal pathogen Candida albicans

Doss,

Moore,

Harman

et al. 2023

PeerJ

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Background Candida albicans is the most prevalent human fungal pathogen. In immunocompromised individuals, C. albicans can cause serious systemic disease, and patients infected with drug-resistant isolates have few treatment options. The ubiquitin-proteasome system has not been thoroughly characterized in C. albicans. Research from other organisms has shown ubiquitination is important for protein quality control and regulated protein degradation at the endoplasmic reticulum (ER) via ER-associated protein degradation (ERAD). Methods Here we perform the first characterization, to our knowledge, of ERAD in a human fungal pathogen. We generated functional knockouts of C. albicans genes encoding three proteins predicted to play roles in ERAD, the ubiquitin ligases Hrd1 and Doa10 and the ubiquitin-conjugating enzyme Ubc7. We assessed the fitness of each mutant in the presence of proteotoxic stress, and we used quantitative tandem mass tag mass spectrometry to characterize proteomic alterations in yeast lacking each gene. Results Consistent with a role in protein quality control, yeast lacking proteins thought to contribute to ERAD displayed hypersensitivity to proteotoxic stress. Furthermore, each mutant displayed distinct proteomic profiles, revealing potential physiological ERAD substrates, co-factors, and compensatory stress response factors. Among candidate ERAD substrates are enzymes contributing to ergosterol synthesis, a known therapeutic vulnerability of C. albicans. Together, our results provide the first description of ERAD function in C. albicans, and, to our knowledge, any pathogenic fungus.

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Characterization of endoplasmic reticulum-associated degradation in the human fungal pathogen Candida albicans

Doss,

Moore,

Harman

et al. 2023

PeerJ

View full text Add to dashboard Cite

show abstract

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Author response: Doa10 is a membrane protein retrotranslocase in ER-associated protein degradation

Cited by 1 publication

References 0 publications

Characterization of endoplasmic reticulum-associated degradation in the human fungal pathogen Candida albicans

Characterization of endoplasmic reticulum-associated degradation in the human fungal pathogen Candida albicans

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