Author response: Endothelial Ca2+ oscillations reflect VEGFR signaling-regulated angiogenic capacity in vivo

Yokota, Yasuhiro; Nakajima, Hiroyuki; Wakayama, Yuki; Muto, Akira; Kikuchi, Kôichi; Fukuhara, Shigetomo; Mochizuki, Naoki

doi:10.7554/elife.08817.038

Cited by 5 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In control embryos, Vegfr-active ECs were abundant and heterogeneously spread throughout the dorsal aorta during early phases of tip cell selection (up to 21 hpf ). This broad and random distribution then rapidly resolved to a refined, highly regular salt and pepper pattern of Vegfr-active ECs by 22 hpf, indicating robust tip cell patterning coincident with initiation of ISV branching, as previously described [73]. Consequently, tip cells that initiated ISV branching were consistently pErk-positive (figure 2f ).…”

Section: Resultssupporting

confidence: 72%

Active perception during angiogenesis: filopodia speed up Notch selection of tip cells in silico and* in vivo*

Zakirov

Charalambous

Thuret

et al. 2021

Phil. Trans. R. Soc. B

View full text Add to dashboard Cite

How do cells make efficient collective decisions during tissue morphogenesis? Humans and other organisms use feedback between movement and sensing known as ‘sensorimotor coordination’ or ‘active perception’ to inform behaviour, but active perception has not before been investigated at a cellular level within organs. Here we provide the first proof of concept in silico / in vivo study demonstrating that filopodia (actin-rich, dynamic, finger-like cell membrane protrusions) play an unexpected role in speeding up collective endothelial decisions during the time-constrained process of ‘tip cell’ selection during blood vessel formation (angiogenesis). We first validate simulation predictions in vivo with live imaging of zebrafish intersegmental vessel growth. Further simulation studies then indicate the effect is due to the coupled positive feedback between movement and sensing on filopodia conferring a bistable switch-like property to Notch lateral inhibition, ensuring tip selection is a rapid and robust process. We then employ measures from computational neuroscience to assess whether filopodia function as a primitive (basal) form of active perception and find evidence in support. By viewing cell behaviour through the ‘basal cognitive lens' we acquire a fresh perspective on the tip cell selection process, revealing a hidden, yet vital time-keeping role for filopodia. Finally, we discuss a myriad of new and exciting research directions stemming from our conceptual approach to interpreting cell behaviour. This article is part of the theme issue ‘Basal cognition: multicellularity, neurons and the cognitive lens’.

show abstract

Section: Resultssupporting

confidence: 72%

Active perception during angiogenesis: filopodia speed up Notch selection of tip cells in silico and* in vivo*

Zakirov

Charalambous

Thuret

et al. 2021

Phil. Trans. R. Soc. B

View full text Add to dashboard Cite

show abstract

“…Following the 'decide then move' time-ordering, rearrangements should presumably be quite regular, with the timing driven by gene expression delays in the Hes1 oscillator. In a related in vivo zebrafish study, Yokota et al [61] asked the temporal question 'When do ECs respond to VEGF?' providing a tantalizing peak into exactly these kind of early selection dynamics.…”

Section: (B) Time-ordering Of Cell Rearrangement Dynamics During Sproutingmentioning

confidence: 99%

“…Finally, the topology of the live-imaged lung explant model was quantified and found to be significantly less branched. It is interesting to note that Sema3E-Plexin-D1 signalling as the intersection between somites and the DA was found to affect the collective dynamics of EC activation [61].…”

Section: Changing the Tempo To Alter Vascular Structure (A) Slowing Down Selection Alters Vascular Branching Densitymentioning

confidence: 99%

The temporal basis of angiogenesis

Bentley

Chakravartula

2017

Phil. Trans. R. Soc. B

View full text Add to dashboard Cite

The process of new blood vessel growth (angiogenesis) is highly dynamic, involving complex coordination of multiple cell types. Though the process must carefully unfold over time to generate functional, well-adapted branching networks, we seldom hear about the time-based properties of angiogenesis, despite timing being central to other areas of biology. Here, we present a novel, time-based formulation of endothelial cell behaviour during angiogenesis and discuss a flurry of our recent, integrated in silico/in vivo studies, put in context to the wider literature, which demonstrate that tissue conditions can locally adapt the timing of collective cell behaviours/decisions to grow different vascular network architectures. A growing array of seemingly unrelated ‘temporal regulators’ have recently been uncovered, including tissue derived factors (e.g. semaphorins or the high levels of VEGF found in cancer) and cellular processes (e.g. asymmetric cell division or filopodia extension) that act to alter the speed of cellular decisions to migrate. We will argue that ‘temporal adaptation’ provides a novel account of organ/disease-specific vascular morphology and reveals ‘timing’ as a new target for therapeutics. We therefore propose and explain a conceptual shift towards a ‘temporal adaptation’ perspective in vascular biology, and indeed other areas of biology where timing remains elusive.This article is part of the themed issue ‘Systems morphodynamics: understanding the development of tissue hardware’.

show abstract

The potential of TRP channels as new prognostic and therapeutic targets against prostate cancer progression

Chinigò,

Ruffinatti,

Munaron

2024

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer

View full text Add to dashboard Cite

Author response: Endothelial Ca2+ oscillations reflect VEGFR signaling-regulated angiogenic capacity in vivo

Cited by 5 publications

References 0 publications

Active perception during angiogenesis: filopodia speed up Notch selection of tip cells in silico and* in vivo*

Active perception during angiogenesis: filopodia speed up Notch selection of tip cells in silico and* in vivo*

The temporal basis of angiogenesis

The potential of TRP channels as new prognostic and therapeutic targets against prostate cancer progression

Contact Info

Product

Resources

About

Author response: Endothelial Ca2+ oscillations reflect VEGFR signaling-regulated angiogenic capacity in vivo

Cited by 5 publications

References 0 publications

Active perception during angiogenesis: filopodia speed up Notch selection of tip cells in silico and in vivo

Active perception during angiogenesis: filopodia speed up Notch selection of tip cells in silico and in vivo

The temporal basis of angiogenesis

The potential of TRP channels as new prognostic and therapeutic targets against prostate cancer progression

Contact Info

Product

Resources

About

Active perception during angiogenesis: filopodia speed up Notch selection of tip cells in silico and* in vivo*

Active perception during angiogenesis: filopodia speed up Notch selection of tip cells in silico and* in vivo*