Author response: Loss of MeCP2 disrupts cell autonomous and autocrine BDNF signaling in mouse glutamatergic neurons

Sampathkumar, Charanya; Wu, Yuan-Ju; Vadhvani, Mayur; Trimbuch, Thorsten; Eickholt, Britta J.; Rosenmund, Christian

doi:10.7554/elife.19374.017

Cited by 2 publications

(1 citation statement)

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“…Corresponding to this Bdnf -glutamate interaction, the modulation of glutamate release likewise depended on Bdnf secretion in oligodendrocytes (Jang et al, 2019). Furthermore, the effects of methyl CpG binding protein 2 alteration toward glutamatergic neurons are conditioned on Bdnf expression (Sampathkumar et al, 2016). Although this work appears tangential to supporting our postulation, it demonstrates the lacuna that exists for fully grasping the detailed mechanisms of glutamatergic signaling in sexual behavior.…”

Section: Potential Role Of Glutamate In Steroid-independent Male Sexumentioning

confidence: 69%

Glutamate in Male and Female Sexual Behavior: Receptors, Transporters, and Steroid Independence

Chiang

Park

2020

Front. Behav. Neurosci.

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The survival of animal species predicates on the success of sexual reproduction. Neurotransmitters play an integral role in the expression of these sexual behaviors in the brain. Here, we review the role of glutamate in sexual behavior in rodents and non-rodent species for both males and females. These encompass the release of glutamate and correlations with glutamate receptor expression during sexual behavior. We then present the effects of glutamate on sexual behavior, as well as the effects of antagonists and agonists on different glutamate transporters and receptors. Following that, we discuss the potential role of glutamate on steroid-independent sexual behavior. Finally, we demonstrate the interaction of glutamate with other neurotransmitters to impact sexual behavior. These sexual behavior studies are crucial in the development of novel treatments of sexual dysfunction and in furthering our understanding of the complexity of sexual diversity. In the past decade, we have witnessed the burgeoning of novel techniques to study and manipulate neuron activity, to decode molecular events at the single-cell level, and to analyze behavioral data. They pose exciting avenues to gain further insight into future sexual behavior research. Taken together, this work conveys the essential role of glutamate in sexual behavior.

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Section: Potential Role Of Glutamate In Steroid-independent Male Sexumentioning

confidence: 69%

Glutamate in Male and Female Sexual Behavior: Receptors, Transporters, and Steroid Independence

Chiang

Park

2020

Front. Behav. Neurosci.

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Functional architecture of the synaptic transducers at a central glutamatergic synapse

Brockmann

Toulmé

Grasskamp

et al. 2020

Preprint

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SummaryNeuronal synapses transduce information via the consecutive action of three transducers: voltage-gated Ca2+-channels, fusion-competent synaptic vesicles, and postsynaptic receptors. Their physical distance is thought to influence the speed and efficiency of neurotransmission. However, technical limitations have hampered resolving their nanoscale arrangement. Here, we developed a new method for live-labeling proteins for electron microscopy (EM), revealing that release-competent vesicles preferentially align with Ca2+-channels and postsynaptic AMPA receptors within 20-30 nm and thereby forming a transsynaptic tripartite nanocomplex. Using functional EM, we show that single action potentials cause vesicles within the nanocomplex to fuse with a 50% probability. The loss of the presynaptic scaffold disrupts the formation of the tripartite transducers. Strikingly, the forced transsynaptic alignment of the Ca2+-channel subunit α2δ1 and AMPA receptors suffice to restore neurotransmission in a scaffold lacking synapse. Our results demonstrate a synaptic transducer nanocomplex that actively contributes to the organization of central synapses.

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Author response: Loss of MeCP2 disrupts cell autonomous and autocrine BDNF signaling in mouse glutamatergic neurons

Cited by 2 publications

References 0 publications

Glutamate in Male and Female Sexual Behavior: Receptors, Transporters, and Steroid Independence

Glutamate in Male and Female Sexual Behavior: Receptors, Transporters, and Steroid Independence

Functional architecture of the synaptic transducers at a central glutamatergic synapse

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