Author response: Replication Study: Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis

Sheen, Mee Rie; Fields, Jennifer; Northan, B.; Lacoste, Judith; Ang, Lay‐Hong; Fiering, Steven; Iorns, Elizabeth; Tsui, Rachel; Denis, Alexandria; Perfito, Nicole; Errington, Tim

doi:10.7554/elife.45120.sa2

Cited by 2 publications

(2 citation statements)

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“…Loss of E-cadherin alteration of the Wnt signaling pathway is an essential process in mesenchymal transition ( 254 ). In the Wnt pathway, β-catenin is responsible for the downregulation of E-cadherin, leading to the mesenchymal transition ( 255 ).…”

Section: Chlamydia Trachomatismentioning

confidence: 99%

Bacteria-Mediated Oncogenesis and the Underlying Molecular Intricacies: What We Know So Far

et al. 2022

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Cancers are known to have multifactorial etiology. Certain bacteria and viruses are proven carcinogens. Lately, there has been in-depth research investigating carcinogenic capabilities of some bacteria. Reports indicate that chronic inflammation and harmful bacterial metabolites to be strong promoters of neoplasticity. Helicobacter pylori-induced gastric adenocarcinoma is the best illustration of the chronic inflammation paradigm of oncogenesis. Chronic inflammation, which produces excessive reactive oxygen species (ROS) is hypothesized to cause cancerous cell proliferation. Other possible bacteria-dependent mechanisms and virulence factors have also been suspected of playing a vital role in the bacteria-induced-cancer(s). Numerous attempts have been made to explore and establish the possible relationship between the two. With the growing concerns on anti-microbial resistance and over-dependence of mankind on antibiotics to treat bacterial infections, it must be deemed critical to understand and identify carcinogenic bacteria, to establish their role in causing cancer.

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Section: Chlamydia Trachomatismentioning

confidence: 99%

Bacteria-Mediated Oncogenesis and the Underlying Molecular Intricacies: What We Know So Far

et al. 2022

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“…So far, 17 replication studies have been completed. Five studies reported the successful reproduction of the original studies [16][17][18][19][20], while eight reported a mixed outcome [21][22][23][24][25][26][27][28], and four failed to reproduce the original findings [29][30][31][32]. It is not clear whether these data are representative.…”

Section: Introductionmentioning

confidence: 98%

Data variability in standardised cell culture experiments

Reddin

Fenton

Wass

et al. 2021

Preprint

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Despite much debate about a perceived 'reproducibility crisis' in the life sciences, it remains unclear what level of replicability is technically possible. Here, we analysed the variation among drug response data of the NCI60 project, which for decades has tested anti-cancer agents in a 60-cell line panel following a standardised protocol. In total, 2.8 million compound/cell line experiments are available in the NCI60 resource CellMiner. The largest fold change between the lowest and highest GI50 (concentration that reduces cell viability by 50%) in a compound/cell line combination was 3.16 x 1010. All compound/ cell line combinations with >100 experiments displayed maximum GI50 fold changes >5, 99.7% maximum fold changes >10, 87.3% maximum fold changes >100, and 70.5% maximum fold changes >1000. FDA-approved drugs and experimental agents displayed similar variation. The variability remained very high after removal of outliers and among experiments performed in the same month. Hence, our analysis shows that high variability is an intrinsic feature of experimentation in biological systems, even among highly standardised experiments in a world-leading research environment. Thus, a narrow focus on experiment standardisation does not ensure a high level of replicability on its own.

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Author response: Replication Study: Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis

Cited by 2 publications

References 0 publications

Bacteria-Mediated Oncogenesis and the Underlying Molecular Intricacies: What We Know So Far

Bacteria-Mediated Oncogenesis and the Underlying Molecular Intricacies: What We Know So Far

Data variability in standardised cell culture experiments

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