Auto‐amplification system for prostaglandin F2α in bovine corpus luteum

Kumagai, Asuka; Yoshioka, Shin; Sakumoto, Ryosuke; Okuda, Kiyoshi

doi:10.1002/mrd.22332

Cited by 9 publications

(7 citation statements)

References 43 publications

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“…This auto-amplification loop system for PGF 2α production may aid in the progression towards CL luteolysis. Enzymes such as PTGS2 and PTGFS are known to participate in PGF 2α synthesis [ 56 , 57 ]. Shirasuna et al [ 56 ] confirmed that the mRNA expression of key enzymes of PGF 2α biosynthesis was increased in the bovine CL after PGF 2α treatment.…”

Section: Discussionmentioning

confidence: 99%

The Role of Peroxisome Proliferator-Activated Receptors in PGF2α-Induced Luteolysis in the Bovine Corpus Luteum

Socha

Łada²,

Jończyk

et al. 2022

Animals

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The participation of peroxisome proliferator-activated receptors (PPARs) in ovarian function in cattle is still not fully understood. The aim of this in vitro study was to determine: (i) the immunolocalization, mRNA expression and tissue concentration of PPARα, PPARδ and PPARγ in the bovine corpus luteum (CL) (n = 40) throughout the estrous cycle, and (ii) the involvement of PPAR in PGF2α-induced processes related to luteolysis. CL (n = 9) explants were cultured in the presence of PPAR antagonists (10−5 M) in combination with or without PGF2α receptor antagonist (10−5 M) and PGF2α (10−6 M). The mRNA and protein expression of PPARs was evaluated through qPCR, IHC, and ELISA, respectively. The results showed that PPAR mRNA and protein expression differed according to the luteal stages. PGF2α upregulated PPARδ and PPARγ mRNA expression in the bovine CL in vitro, whereas PPARγ increased the inhibitory effect of PGF2α by decreasing progesterone secretion and the mRNA expression of hydroxy-delta-5-steroid dehydrogenase, 3 β- and steroid delta-isomerase 1 (HSD3B1) in the CL explants; mRNA transcription of tumor necrosis factor α (TNFα) and inducible nitric oxide synthase (iNOS) was increased. The obtained results indicate that the mRNA and protein expression of PPARs changes in the bovine CL throughout the estrous cycle and under the influence of PGF2α. We suggest that isoform γ, among all examined PPARs, could be a factor involved in the regulation of PGF2α-induced processes related to luteolysis in the bovine CL. Further studies are needed to understand the role of PPAR in luteal regression in the CL of cattle.

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Section: Discussionmentioning

confidence: 99%

The Role of Peroxisome Proliferator-Activated Receptors in PGF2α-Induced Luteolysis in the Bovine Corpus Luteum

Socha

Łada²,

Jończyk

et al. 2022

Animals

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“…PGE 2 and PGF 2α have opposing effects, but it has been suggested that their expression is coordinated to achieve a balance in vivo . It has also been proposed that PGF2α significantly stimulated intra-luteal PGF 2α production in all luteal phases, but did not affect PGE2 production [ 28 ]. PGE 2 rapidly increased TbetaRIII mRNA and protein expression and enhanced TbetaRIII gene promoter activity ctivity, whereas PGF 2α does not [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

The prostaglandin E<sub>2</sub> receptor PTGER2 and prostaglandin F<sub>2α</sub> receptor PTGFR mediate oviductal glycoprotein 1 expression in bovine oviductal epithelial cells

Zhang

Mao

Zhang

et al. 2018

Journal of Reproduction and Development

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Oviductal glycoprotein 1 (OVGP1), an oviductin, is involved in the maintenance of sperm viability and motility and contributes to sperm capacitation in the oviduct. In this study, the regulatory effects exerted by prostaglandin E2 (PGE2) and F2α (PGF2α) on OVGP1 expression via their corresponding receptors in bovine oviductal epithelial cells (BOECs) were investigated. BOECs were cultured in vitro, and their expression of receptors of PGE2 (PTGER1, PTGER2, PTGER3, and PTGER4) and PGF2α (PTGFR) was measured using RT-qPCR. Ca2+ concentration was determined with a fluorescence-based method and cAMP was quantified by enzyme-linked immunosorbent assays to verify activation of PTGER2 and PTGFR by their corresponding agonists in these cells. OVGP1 mRNA and protein expression was measured using RT-qPCR and western blotting, respectively, following PTGER2 and PTGFR agonist-induced activation. PTGER1, PTGER2, PTGER4, and PTGFR were found to be present in BOECs; however, PTGER3 expression was not detected. OVGP1 expression was significantly promoted by 10–6 M butaprost (a PTGER2 agonist) and decreased by 10–6 M fluprostenol (a PTGFR agonist). In addition, 3 μM H-89 (a PKA inhibitor) and 3 μM U0126 (an ERK inhibitor) effectively inhibited PGE2-induced upregulation of OVGP1, and 5 μM chelerythrine chloride (a PKC inhibitor) and 3 μM U0126 negated OVGP1 downregulation by PGF2α. In conclusion, this study demonstrates that OVGP1 expression in BOECs is enhanced by PGE2 via PTGER2-cAMP-PKA signaling, and reduced by PGF2α through the PTGFR-Ca2+-PKC pathway.

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“…Since leukocytes are known to produce a variety of cytokines including IFNG and TNF [ 38 ], the uterus and macrophages seem to be sources of PGF and INFG. In addition, we described an auto-amplification system for PGF in the bovine CL [ 39 ]. Luteal PGF may also play a role in regulating MMP-1 and TIMP expression during luteolysis.…”

Section: Discussionmentioning

confidence: 99%

Expression of matrix metalloproteinases in bovine luteal cells induced by prostaglandin F2α, interferon γ and tumor necrosis factor α

Abe

Sakumoto

Okuda

2015

Journal of Reproduction and Development

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We recently demonstrated that luteal cells flow out from the ovary via lymphatic vessels during luteolysis. However, the regulatory mechanisms of the outflow of luteal cells are not known. Matrix metalloproteinases (MMPs) can degrade the extracellular matrix and basal membrane, and tissue inhibitors of matrix metalloproteinases (TIMPs) inhibit the activity of MMPs. To test the hypothesis that MMP expression in luteal cells is regulated by luteolytic factors, we investigated the effects of prostaglandin F2α (PGF), interferon γ (IFNG) and tumor necrosis factor α (TNF) on the mRNA expression of MMPs and TIMPs in cultured luteal cells. Luteal cells obtained from the CL at the mid-luteal stage (days 8–12 after ovulation) were cultured with PGF (0.01, 0.1, 1 μM), IFNG (0.05, 0.5, 5 nM) and TNF (0.05, 0.5, 0.5 nM) alone or in combination for 24 h. PGF and IFNG significantly increased the expression of MMP-1 mRNA. In addition, 1 μM PGF in combination with 5 nM IFNG stimulated MMP-1 and MMP-9 mRNA expression significantly more than either treatment alone. In contrast, IFNG significantly decreased the level of MMP-14 mRNA. The mRNA expression of TIMP-1, which preferentially inhibits MMP-1, was suppressed by 5 nM INFG. One μM PGF and 5 nM IFNG suppressed TIMP-2 mRNA expression. These results suggest a new role of MMPs: luteal MMPs stimulated by PGF and IFNG break down the extracellular matrix surrounding luteal cells, which accelerates detachment from the CL during luteolysis, providing an essential prerequisite for outflow of luteal cells from the CL to lymphatic vessels.

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Auto‐amplification system for prostaglandin F2α in bovine corpus luteum

Cited by 9 publications

References 43 publications

The Role of Peroxisome Proliferator-Activated Receptors in PGF2α-Induced Luteolysis in the Bovine Corpus Luteum

The Role of Peroxisome Proliferator-Activated Receptors in PGF2α-Induced Luteolysis in the Bovine Corpus Luteum

The prostaglandin E<sub>2</sub> receptor PTGER2 and prostaglandin F<sub>2α</sub> receptor PTGFR mediate oviductal glycoprotein 1 expression in bovine oviductal epithelial cells

Expression of matrix metalloproteinases in bovine luteal cells induced by prostaglandin F2α, interferon γ and tumor necrosis factor α

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