Auto Anti-M: Clinical and Serologic Findings of Seven Patients from One Hospital and Review of the Literature

Sacher, Ronald A.; Abbondanzo, Susan L.; Miller, Deborah K.; Womack, Betty

doi:10.1093/ajcp/91.3.305

Cited by 10 publications

(5 citation statements)

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“…In fact, in this study, the average age of patients studied with auto‐anti‐M was 73 years old while the average age of patients with allo‐anti‐M/N was 51 years old. Nevertheless, the incidence of auto‐anti‐M is low with one case series of seven patients with auto‐anti‐M reported in the literature by Sacher et al 21 In this case series, the patients had a variety of clinical features and diagnoses for which one could not identify a common cause accounting for the development of an auto‐anti‐M 21 …”

Section: Discussionmentioning

confidence: 90%

Autoantibodies to red blood cell surface Glycophorin A impact the activation poise of circulating leukocytes

et al. 2021

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Background Both M and N alleles encode antigens on Glycophorin A (GPA), a red blood cell (RBC) surface sialoglycoprotein. Interaction between RBC GPA and leukocyte surface lectins may downregulate their activation. The current study investigates if RBC autoantibodies against GPA, such as auto‐anti‐M/N, prime an activated phenotype in peripheral blood leukocytes. Methods Leukocyte activation was assessed in whole blood from patients with auto‐anti‐GPA (anti‐M/N) and compared to those with allo‐anti‐M/N and healthy subjects. Control samples from healthy subjects with no antibodies incubated in vitro with either anti‐GPA or anti‐Rh were analyzed for neutrophil and monocyte surface activation marker expression, reactive oxygen species (ROS) content, and formation of aggregates with RBCs. Samples incubated with an IgG1 isotype antibody served as controls. Results Ex vivo, neutrophil CD66b and monocyte CD63 surface expression was increased in patients with auto‐anti‐M/N compared to those with allo anti‐M/N (p = .1757; p = .0698) and to healthy subjects (p = .0186; p = .013). In vitro, neutrophil CD66b and monocyte CD63 surface expression was increased following incubation with anti‐GPA compared to anti‐Rh (p = .0003; p = .0328) and isotype control (p = .000; p = .0062). Intracellular ROS content increased in both neutrophils and monocytes incubated with anti‐GPA compared to anti‐Rh (p = .0012; p = .0693) and isotype control (p = .001; p = .0021). Percentage of neutrophil‐RBC aggregates was decreased when incubated with anti‐GPA compared to isotype control (p < .01). Conclusions Neutrophils and monocytes in peripheral blood exposed to an antibody directed against GPA on RBC surfaces, such as M or N antigens, may be primed towards an activated phenotype.

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Section: Discussionmentioning

confidence: 90%

Autoantibodies to red blood cell surface Glycophorin A impact the activation poise of circulating leukocytes

et al. 2021

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“…At least 15 cases of patients with autoanti-M have been reported and reviewed by Sacher et al . [ 11 ] According to him, 11 were non significant while rest four gave some symptoms of cold hemagglutinin disease. Anti–M may show a dosage effect as in the case of the female patient, reacting more strongly with homozygous cells (M+N-) than cells (M+N+).…”

Section: Discussionmentioning

confidence: 99%

Anti-M: Report of two cases and review of literature

Tondon

Kataria

Chaudhry

2008

Asian J Transfus Sci

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Anti-M is a fairly common naturally occurring antibody with rarely causing hemolytic transfusion reactions or hemolytic disease of the newborn. Most anti-M are not active at 37°C and can generally be ignored in transfusion practice. However, we did not find this antibody to be fairly common and detected only two cases of anti-M in the past three years. We describe these two cases; one ‘immunizing’ type and other ‘naturally occurring’ and review the literature. The immunizing type was reactive at 37°C as well as AHG phase of testing with IgG component, and showing dosage effect while the other was ‘naturally occurring’ reactive well below 37°C. Though rare, sometimes these antibodies can be of clinical significance when the antibody detected is reactive at 37°C and AHG phase. When the antibody is active at 37°C, M antigen negative cross match compatible red cell unit should be given.

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“…This finding is in contrast to our finding from the whole blood donor, where the anti-M was reactive at room temperature with optimum activity at 4°C. Sacher et al 12 described 17 cases of autoanti-M, of which four had symptoms of cold agglutinin disease, such as Raynaud's phenomenon, livedo reticularis, and acrocyanosis, and two had mild hemolysis; the rest were asymptomatic despite having a significant titer of the autoanti-M. Patients with anti-M have been described by Tandon et al 13 and Kaur et al 14 These antibodies were identified as IgM, causing ABO grouping discrepancy, with optimum reactivity below 37°C.…”

Section: Discussionmentioning

confidence: 99%

Clinical and laboratory profile of anti-M

Basu

Basu²,

Reddy³

et al. 2017

Immunohematology

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Anti-M is a frequently detected naturally occurring antibody that has been reported in various clinical settings and also in voluntary donors. We describe here the clinical and laboratory findings of 11 cases with anti-M detected at our center. This report is a retrospective study in which we reviewed our immunohematology laboratory records for cases involving anti-M. Both donor and patient data from a 28-month period (September 2014 to December 2016) were reviewed. During this period, 11 examples of anti-M were detected (8 patients, 1 voluntary whole blood donor, and 1 hematopoietic stem cell donor. Anti-M was also detected in one external quality assessment scheme sample received during this period. In conclusion, anti-M can be detected in various clinical settings. This antibody can be clinically significant; in the laboratory, it can present as a serologic problem such as an ABO group discrepancy or an incompatible crossmatch. After detection, management and course of action is determined by both the antibody characteristics and the clinical setting.

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Auto Anti-M: Clinical and Serologic Findings of Seven Patients from One Hospital and Review of the Literature

Cited by 10 publications

References 0 publications

Autoantibodies to red blood cell surface Glycophorin A impact the activation poise of circulating leukocytes

Autoantibodies to red blood cell surface Glycophorin A impact the activation poise of circulating leukocytes

Anti-M: Report of two cases and review of literature

Clinical and laboratory profile of anti-M

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