Auto-fluorescent PAMAM-based dendritic molecules and their potential application in pharmaceutical sciences

Abstract

The epithelial permeation of water-soluble fluorescent PAMAM dendrons based on 7Hbenz[de] benzimidazo [2,1-a] isoquinoline-7-one as a fluorescent core across epithelial cell models MDCK I and MDCK II has been quantified.Hydrodynamic radii have been derived from self-diffusion coefficients obtained via pulsedgradient spin-echo Nuclear Magnetic Resonance (PGSE-NMR). Results indicate that these dendritic molecules are molecularly disperse, non-aggregating, and only slightly larger than their parent homologues. MDCK I permeability studies across epithelial barriers show that these dendritic molecules are biocompatible with the chosen epithelial in-vitro model and can permeate across MDCK cell monolayers. Permeability is demonstrated to be a property of dendritic size and cell barrier restrictiveness indicating that paracellular mechanisms play the predominant role in the transport of these molecules.

Recent advances in development of dendritic polymer‐based nanomedicines for cancer diagnosis

“…For example,7H‐benz benzimidazo[2,1‐α] isoquinoline‐7‐one was applied as a fluorescent core of water‐soluble PAMAM dendrons. Their non‐aggregation behaviors have been further confirmed by their self‐diffusion coefficients and insensitivity to variations in the concentration range (0.83 ~ 5.75 × 10 −4 mM) with the aid of pulsed‐gradient spin‐echo nuclear magnetic resonance (El‐Betany et al, 2020).…”

“…Recent decades have witnessed rapid development of dendritic polymers in the field of biomedicine, especially in cancer diagnosis. Dendritic polymers, including dendrons, dendrimers, hyperbranched polymers, dendrigraft polymers and other dendritic hybrid polymers (Cook & Perrier, 2020), have emerged to deliver imaging probes or function as imaging reporters (El‐Betany et al, 2020; Jin et al, 2018; Y. Zeng, Li, et al, 2020), as well as capture cancer markers, such as circulating tumor cells (CTCs), cell‐free tumor DNA (ctDNA), and tumor derived exosomes (Bu et al, 2020; J. Dong et al, 2020; Poellmann et al, 2020). The intrinsic physiochemical properties endow the dendritic polymers with unique advantages in cancer diagnosis, and the use of dendritic polymer‐based nanomedicines is of great help in effectively diagnosing early stage tumors and tracking the information on tumor characterization, invasiveness, metastasis, and prognosis (C. Li, Wang, et al, 2020; Tan et al, 2020).…”

“…Recent decades have witnessed rapid development of dendritic polymers in the field of biomedicine, especially in cancer diagnosis. Dendritic polymers, including dendrons, dendrimers, hyperbranched polymers, dendrigraft polymers and other dendritic hybrid polymers (Cook & Perrier, 2020), have emerged to deliver imaging probes or function as imaging reporters (El-Betany et al, 2020;Jin et al, 2018;, as well as capture cancer markers, such as circulating tumor cells (CTCs), cell-free tumor DNA (ctDNA), and tumor derived exosomes (Bu et al, 2020;J. Dong et al, 2020;Poellmann et al, 2020).…”

Intrinsic Blue Fluorescence of 2.0G PAMAM-DCM Polymer Dots and Its Applications for Fe3+ Sensing

“…These studies are helpful to gain a better understanding of the relationship between the internal backbone and fluorescence. Recent studies have concluded that the fluorescence properties of PAMAM will be affected by special conditions, including the oxidation of tertiary amines, photoelectron transfer, and the aggregation of internal carbonyl groups [15][16][17]. This indicates that at least two fluorescence sites are inside the PAMAM, influenced by pH or temperature [18][19][20][21].…”