Auto IgG anti-IgE and IgG × IgE immune complex presence and effects on ELISA-based quantitation of IgE in canine atopic dermatitis, demodectic acariasis and helminthiasis

Hammerberg, Bruce; Bevier, Diane E.; DeBoer, Douglas J.; Olivry, Thierry; Orton, Susan M.; Gebhard, David F.; Vaden, Shelly L.

doi:10.1016/s0165-2427(97)00119-0

Cited by 39 publications

(23 citation statements)

References 19 publications

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“…Even if free IgE were neutralized to 99% by the anti-IgE, the therapy still would fail because the few remaining IgE molecules would be sufficient to sensitise the mast cells and basophils [29]. This would be a more serious problem in dogs since levels of IgE are relatively high compared to humans [28]. However, an anti-IgE approach has the potential to provide therapeutic efficacy through other actions.…”

Section: Discussionmentioning

confidence: 99%

“…[28]. Plates were coated with polyclonal anti-dog IgE, and captured IgE was detected by monoclonal antibodies specific for heat-stable IgE epitopes, as described [28].…”

Section: Measurement Of Anti-ige Antibodiesmentioning

confidence: 99%

“…The sera collected from two responsive dogs from each of groups 1-3 and a dog from control group 5 were assayed for total IgE content, including free IgE and IgE within anti-IgE/IgE immune complexes, by the quantitative assay on heated serum [28]. Serum IgE for responsive dogs from group 1 (nos.…”

Section: In Vivo Functional Immunogenicity In Dogsmentioning

confidence: 99%

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Synthetic IgE peptide vaccine for immunotherapy of allergy

Wang¹

2003

Vaccine

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Section: Discussionmentioning

confidence: 99%

“…[28]. Plates were coated with polyclonal anti-dog IgE, and captured IgE was detected by monoclonal antibodies specific for heat-stable IgE epitopes, as described [28].…”

Section: Measurement Of Anti-ige Antibodiesmentioning

confidence: 99%

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Synthetic IgE peptide vaccine for immunotherapy of allergy

Wang¹

2003

Vaccine

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“…The canine sera were serially twofold diluted starting at a dilution of 1∶100. After incubation and washing, biotinylated monoclonal anti-canine IgE antibody 5.91 [39] was added for 1 h, followed by extravidine-alkaline phosphatase (Sigma). The plates were then developed with a of phosphatase substrate (Sigma) in diethanolamine (Fluka) solution.…”

Section: Methodsmentioning

confidence: 99%

Two Loci on Chromosome 5 Are Associated with Serum IgE Levels in Labrador Retrievers

et al. 2012

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Crosslinking of immunoglobulin E antibodies (IgE) bound at the surface of mast cells and subsequent mediator release is considered the most important trigger for allergic reactions. Therefore, the genetic control of IgE levels is studied in the context of allergic diseases, such as asthma, atopic rhinitis, or atopic dermatitis (AD). We performed genome-wide association studies in 161 Labrador Retrievers with regard to total and allergen-specific immunoglobulin E (IgE) levels. We identified a genome-wide significant association on CFA 5 with the antigen-specific IgE responsiveness to Acarus siro. We detected a second genome-wide significant association with respect to the antigen-specific IgE responsiveness to Tyrophagus putrescentiae at a different locus on chromosome 5. A. siro and T. putrescentiae both belong to the family Acaridae and represent so-called storage or forage mites. These forage mites are discussed as major allergen sources in canine AD. No obvious candidate gene for the regulation of IgE levels is located under the two association signals. Therefore our studies offer a chance of identifying a novel mechanism controlling the host's IgE response.

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“…14 Thus, biotinylated human recombinant FcεRI alpha chain (R&D Systems; Minneapolis, MN, USA) and biotinylated scFv were used to detect plasma IgE, with the former being conventionally referred to in human studies with omalizumab as detecting "free" IgE (not complexed with omalizumab or FcεRI alpha chain), and the latter being referred to as "total" IgE based on scFv binding at a site not blocked by the FcεRI alpha chain. Absolute values of IgE lg/ml are reported based on standard curves using affinity purified monoclonal canine IgE; however, the use of low pH elution of the IgE standard from affinity matrix reduces the signal values from biotinylated FcεRI alpha chain detection, which precludes direct comparison of the plasma values generated by the two different protocols.…”

Section: Cd23mentioning

confidence: 99%

Therapeutic anti‐IgE monoclonal antibody single chain variable fragment (scFv) safety and immunomodulatory effects after one time injection in four dogs

Hammerberg

Eguiluz-Hernandez

2017

Advances in Veterinary Dermatology

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Background -The therapeutic monoclonal antibody omalizumab that is specific for IgE has proven to be an effective addition to the treatment of allergic disease in humans.Hypothesis/Objectives -The aims of this study were to demonstrate the safety and immunomodulating effects of a single injection of a monoclonal antibody single chain variable fragments (scFv) specific for canine IgE in normal dogs.Animals -Three normal dogs were bled for EDTA whole blood samples for 112 days post-injection (dpi). A fourth dog was monitored for 28 days.Methods -Anti-IgE scFv was pegylated to minimize scFv dimerization. Four normal dogs were injected once subcutaneously with anti-IgE scFv at 1 mg/kg. Flow cytometry was performed on whole blood. Plasma levels of IgE were measured by ELISA.Results -None of the four dogs showed signs of anaphylaxis. All dogs demonstrated decreases in IgE(+) cells in lymphocyte-gated events by 14 dpi. Dogs C and D returned to pre-injection levels by 21 days, whereas dogs A and B remained below pre-injection levels until Day 112. Similar differences were seen in IgE-bearing granulocyte-gated cells. Free plasma IgE decreased below pre-injection levels by 47% in Dog A and by 52% in Dog B at 112 days. Dogs C and D did not change by more than 32% from preinjection levels.Conclusion -A single injection of monomeric, pegylated scFv with high affinity for dog IgE was demonstrated to be safe. Marked reduction in IgE-bearing lymphocytes and granulocytes accompanied by reduced "free" plasma IgE level in two of four dogs is analogous to omalizumab in humans.

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Auto IgG anti-IgE and IgG × IgE immune complex presence and effects on ELISA-based quantitation of IgE in canine atopic dermatitis, demodectic acariasis and helminthiasis

Cited by 39 publications

References 19 publications

Synthetic IgE peptide vaccine for immunotherapy of allergy

Synthetic IgE peptide vaccine for immunotherapy of allergy

Two Loci on Chromosome 5 Are Associated with Serum IgE Levels in Labrador Retrievers

Therapeutic anti‐IgE monoclonal antibody single chain variable fragment (scFv) safety and immunomodulatory effects after one time injection in four dogs

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