Auto-regulation of estrogen receptor subtypes and gene expression profiling of 17β-estradiol action in the neuroendocrine axis of male goldfish

Marlatt, Vicki L.; Zhang, Dapeng; Xiong, Hua; Watt, Jennifer; Xia, Xuhua; Moon, Thomas W.; Trudeau, Vance L.

doi:10.1016/j.mce.2007.10.013

Cited by 113 publications

(81 citation statements)

References 51 publications

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“…This assumption is reinforced by the fact that treatment of control embryos with ICI causes a strong reduction in cypa19a1b basal expression at all time points suggesting that basal expression relies in large part upon activation of estrogen receptors. We and others have previously shown that the cyp19a1b gene is very sensitive to E2 in young and adult fish (Hinfray et al, 2006;Sawyer et al, 2006;Cheshenko et al, 2007;Lassiter and Linney, 2007;Cheshenko et al, 2008;Le Page et al, 2008;Marlatt et al, 2008;Mouriec et al, 2008;Strobl-Mazzulla et al, 2008 , cyp19a1b mRNAs remained undetectable in the brain using whole-mount in situ hybridization at all these early stages. Nevertheless, as already documented Mouriec et al, 2009), treating zebrafish embryos with E2 causes a dramatic increase in cyp19a1b expression that becomes detectable at the messenger level by in situ hybridization or protein level by immunohistochemistry as early as 32-36 hpf (data not shown).…”

Section: Early Cyp19a1b Expression Relies On Estrogen Receptorsmentioning

confidence: 67%

Early regulation of brain aromatase (cyp19a1b) by estrogen receptors during zebrafish development

Mouriec

Lareyre

Tong

et al. 2009

Developmental Dynamics

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Section: Early Cyp19a1b Expression Relies On Estrogen Receptorsmentioning

confidence: 67%

Early regulation of brain aromatase (cyp19a1b) by estrogen receptors during zebrafish development

Mouriec

Lareyre

Tong

et al. 2009

Developmental Dynamics

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“…25,26 However, compared with the general positive responses observed along the HPGL axis, the transcriptions of subtypes of ER (ERα, ERβ, and ERγ) were differentially down-regulated in the male testis and liver but without changes in the male brain and female tissues, which could be attributed to the tissue-and sex-specific expressions because of the autoregulatory capabilities of the ER subtypes against estrogen mimics to reduce the responsiveness of target tissues to abnormally elevated hormone levels. 27 The histological examination revealed that oogenesis-related processes were not blocked in the female ovary, as verified by the mildly but nonsignificantly decreased fecundity. The incongruity between molecular events and egg production is supposed to result from the complexity of HPGL axis and regulatory network as well as the timing of spawning pattern.…”

Section: ■ Discussionmentioning

confidence: 89%

Chronic Exposure of Marine Medaka (Oryzias melastigma) to 4,5-Dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) Reveals Its Mechanism of Action in Endocrine Disruption via the Hypothalamus-Pituitary-Gonadal-Liver (HPGL) Axis

Chen

Zhang

Rui³

et al. 2016

Environ. Sci. Technol.

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In this study, marine medaka (Oryzias melastigma) were chronically exposed for 28 days to environmentally realistic concentrations of 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) (0, 0.76, 2.45, and 9.86 μg/L), the active ingredient in commercial antifouling agent SeaNine 211. Alterations of the hypothalamus-pituitary–gonadal-liver (HPGL) axis were investigated across diverse levels of biological organization to reveal the underlying mechanisms of its endocrine disruptive effects. Gene transcription analysis showed that DCOIT had positive regulatory effects mainly in male HPGL axis with lesser extent in females. The stimulated steroidogenic activities resulted in increased concentrations of steroid hormones, including estradiol (E2), testosterone (T), and 11-KT-testosterone (11-KT), in the plasma of both sexes, leading to an imbalance in hormone homeostasis and increased E2/T ratio. The relatively estrogenic intracellular environment in both sexes induced the hepatic synthesis and increased the liver and plasma content of vitellogenin (VTG) or choriogenin. Furthermore, parental exposure to DCOIT transgenerationally impaired the viability of offspring, as supported by a decrease in hatching and swimming activity. Overall, the present results elucidated the estrogenic mechanisms along HPGL axis for the endocrine disruptive effects of DCOIT. The reproductive impairments of DCOIT at environmentally realistic concentrations highlights the need for more comprehensive investigations of its potential ecological risks.

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“…RNA was extracted from eight pools consisting of four or five individual hypothalami or telencephali from control and fluoxetine-injected female goldfish. The multiplex reaction for the endogenous reference gene ␤-actin in combination with ER␣, ER␤1, and ER␤2 has been validated by our lab (33). Data were normalized to ␤-actin because the levels did not change significantly with fluoxetine treatment (P ϭ 0.56 hypothalamus; P ϭ 0.99 telencephalon).…”

Section: Methodsmentioning

confidence: 99%

“…We previously described and validated the production and use of our goldfish-carp cDNA microarray (33,34). For a detailed description of the microarray the reader is directed to Williams et al (68).…”

Section: Methodsmentioning

confidence: 99%

Effects of fluoxetine on the reproductive axis of female goldfish (Carassius auratus)

Mennigen

Martyniuk

Crump

et al. 2008

Physiological Genomics

129

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. Effects of fluoxetine on the reproductive axis of female goldfish (Carassius auratus). Physiol Genomics 35: 273-282, 2008. First published September 2, 2008; doi:10.1152/physiolgenomics.90263.2008.-We investigated the effects of fluoxetine, a selective serotonin reuptake inhibitor, on neuroendocrine function and the reproductive axis in female goldfish. Fish were given intraperitoneal injections of fluoxetine twice a week for 14 days, resulting in five injections of 5 g fluoxetine/g body wt. We measured the monoamine neurotransmitters serotonin, dopamine, and norepinephrine in addition to their metabolites with HPLC. Homovanillic acid, a metabolite in the dopaminergic pathway, increased significantly in the hypothalamus. Plasma estradiol levels were measured by radioimmunoassay and were significantly reduced approximately threefold after fluoxetine treatment. We found that fluoxetine also significantly reduced the expression of estrogen receptor (ER)␤1 mRNA by 4-fold in both the hypothalamus and the telencephalon and ER␣ mRNA by 1.7-fold in the telencephalon. Fluoxetine had no effect on the expression of ER␤2 mRNA in the hypothalamus or telencephalon. Microarray analysis identified isotocin, a neuropeptide that stimulates reproductive behavior in fish, as a candidate gene affected by fluoxetine treatment. Real-time RT-PCR verified that isotocin mRNA was downregulated approximately sixfold in the hypothalamus and fivefold in the telencephalon. Intraperitoneal injection of isotocin (1 g/g) increased plasma estradiol, providing a potential link between changes in isotocin gene expression and decreased circulating estrogen in fluoxetine-injected fish. Our results reveal targets of serotonergic modulation in the neuroendocrine brain and indicate that fluoxetine has the potential to affect sex hormones and modulate genes involved in reproductive function and behavior in the brain of female goldfish. We discuss these findings in the context of endocrine disruption because fluoxetine has been detected in the environment. brain; estrogen receptors; isotocin; microarray; Prozac FLUOXETINE (PROZAC), a selective serotonin reuptake inhibitor (SSRI) (46), has been used in several studies in fish to investigate the serotonergic modulation of the endocrine system (55). A racemic mixture of two lipophilic enantiomers, fluoxetine is metabolized by cytochrome P-450 isoenzymes to the active metabolite norfluoxetine (46). In humans, fluoxetine is primarily excreted in urine as ϳ20%-30% unchanged parent compound, while metabolites are largely excreted as pharmacologically active norfluoxetine and inactive fluoxetine glucuronide (62).Fluoxetine also has effects on reproduction in several vertebrates. For example, in the goldfish (Carassius auratus), combined intraperitoneal administration of 10 g/g fluoxetine and 10 g/g serotonin (5-HT) elevated luteinizing hormone (LH) levels relative to 5-HT administration alone 2 h after treatment (55). Japanese medaka (Oryzias latipes) exposed to waterborne 0.1 and 0.5 g/l fluoxetine for 4 wk sh...

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Auto-regulation of estrogen receptor subtypes and gene expression profiling of 17β-estradiol action in the neuroendocrine axis of male goldfish

Cited by 113 publications

References 51 publications

Early regulation of brain aromatase (cyp19a1b) by estrogen receptors during zebrafish development

Early regulation of brain aromatase (cyp19a1b) by estrogen receptors during zebrafish development

Chronic Exposure of Marine Medaka (Oryzias melastigma) to 4,5-Dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) Reveals Its Mechanism of Action in Endocrine Disruption via the Hypothalamus-Pituitary-Gonadal-Liver (HPGL) Axis

Effects of fluoxetine on the reproductive axis of female goldfish (Carassius auratus)

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