Autoantibodies against cerebral muscarinic cholinoceptors in Sjögren syndrome: functional and pathological implications

Reina, Silvia; Sterin‐Borda, Leonor; Orman, Betina; Borda, Enri

doi:10.1016/j.jneuroim.2004.01.019

Cited by 31 publications

(27 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Purification of human IgG: The serum IgG fraction from patients with pSS and from normal individuals (control) was isolated using protein G affinity chromatography as described elsewhere [9]. Briefly, sera were loaded onto the protein G affinity column (Sigma-Aldrich, St Louis, MO, USA) equilibrated with 1 M Tris-HCl (pH 8.0) and the columns were washed with 10 volumes of the same buffer.…”

Section: Serological Studiesmentioning

confidence: 99%

“…Anti-M 3 peptide IgG procedure: The IgG fraction from 26 patients with pSS and 24 healthy subjects was independenthly subjected to affinity chromatography on the synthesized peptide covalently linked to AfiGel 15 gel (Bio-Rad, Richmond, CA,USA) as described by Reina et al [9]. Briefly, the IgG fraction …”

Section: Serological Studiesmentioning

confidence: 99%

“…They are therefore usually used as biomarkers for the classification and diagnosis of pSS and aSS [4] [5]. However, other autoantibodies not currently used in clinical practice such as anti-M 3 muscarinic acetylcholine receptor (anti-M 3 mAChR), have also been described in pSS/aSS [6] [7] [8] as useful markers [9] [10].…”

mentioning

confidence: 99%

See 2 more Smart Citations

Role of M<sub>3</sub> Muscarinic Acethylcholine Receptor Antibodies as a New Marker in Primary Sjögren Syndrome

Reina¹,

Pisoni²,

Arana³

et al. 2017

Self Cite

View full text Add to dashboard Cite

Aims: This paper investigates the presence of M 3 muscarinic acetylcholine receptor autoantibody present in the serum of patients with primary Sjögren syndrome (pSS). Main methods: We detected the levels of M 3 mAChR peptide IgG, PGE 2 , IL-1β in serum of SS patients using the enzyme-linked immune sorbent assay (ELISA). To measure the quantity of nitrite/nitrate, we used Griess reagent system. Key findings: Titres of M 3 mAChR antibody in sera from SS patients are significantly enhanced compared to healthy subjects (control). The enhancement of these autoantibodies is accompanied by the increase of the levels of PGE 2 , IL-1β and nitrite/nitrate in serum. Under in vitro conditions, the synthetic human M 3 peptide impaires the increment of M 3 mAChR antibody but not that of nati-Ro/SSA antibody. In positive anti-Ro/SSA antibody patients, the increment of M 3 mAChR peptide IgG and the measured pro-inflammatory substances is related. Significance: On this basis, anti M 3 mAChR peptide IgG can be said to act as a modulator of the immune system and to play a role in the host-chronic increment of proinflammatory substances in SS patients with positive Ro/SSA antibody. This association between the antibody and the pathogenesis of SS disease may result in useful predicting SS.

show abstract

Section: Serological Studiesmentioning

confidence: 99%

Section: Serological Studiesmentioning

confidence: 99%

See 1 more Smart Citation

Role of M<sub>3</sub> Muscarinic Acethylcholine Receptor Antibodies as a New Marker in Primary Sjögren Syndrome

Reina¹,

Pisoni²,

Arana³

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…genetically and immunological abnormalities, but also the deregulation among them as well as the dysfunction of the parasympathetic system. They have the following cardinal clinical symptoms: xerostomia and xerophtalmia at the level of the exocrine glandular system [1], cognitive impairments such as perception, attention and executive function deficits [2] at the level of the cerebral frontal cortex, complete congenital heart block at the level of neonatal myocardium [3]. These alterations are reliable predictors of long term disabilities.…”

Section: Introductionmentioning

confidence: 99%

“…In this article we will examine the role of cholinergic autoantibodies subtypes M 3 and M 1 and its relationship with the signs and symptoms of SS and its pathological implications in SS.…”

Section: Introductionmentioning

confidence: 99%

Autoantibodies against Muscarinic Acetylcholine Receptor on Exocrine Glands in Sjögren Syndrome

Reina

Borda²

2014

Dentistry

View full text Add to dashboard Cite

These investigations demonstrate that serum antibodies against muscarinic acetylcholine receptors (mAChR) in primary Sjögren syndrome (pSS) and associated Sjögren syndrome (aSS) bind and activate both cholinergic receptors of M 3 in salivary gland and M 1 in neonatal myocardium and in the cerebral frontal cortex area subtypes; triggering the production of the second messengers and proinflammatory mediators related to mAChR activation. In this way the cholinergic autoantibodies damages these receptors, which thus starts acting as an antigen. On this basis M 3 and M 1 mAChR IgG can be considered new markers of pSS/aSS allowing the differentiation between dry eye and mouth of autoimmune and non-autoimmune nature. Given that cholinergic autoantibodies also deregulate the parasympathetic system of the target organs, they can also be seen as a new factor contributing to the etiopathology of the syndrome.

show abstract

Atorvastatin inhibits the inflammatory response caused by anti-M3 peptide IgG in patients with primary Sjögren’s syndrome

et al. 2012

View full text Add to dashboard Cite

Experimental and clinical investigations have revealed that statins can down-regulate acute and chronic inflammatory processes. Whether statins express anti-inflammatory activities in the salivary glands in patients with primary Sjögren's syndrome (pSS) is not known. The in vitro and in vivo effect of atorvastatin on rat submandibular gland treated with anti-M(3) peptide IgG purified from SS patients was studied. The anti-inflammatory effects of atorvastatin were assessed by measuring the levels of IL-1β, PGE(2) and MMP-3 by ELISA. Atorvastatin inhibited the increase in the production of IL-1β, PGE(2) and MMP-3 in submandibular glands treated with anti-M(3) peptide IgG. A positive correlation between IL-1β production with accumulation of PGE(2) and MMP-3 was observed. Rats pre-treated orally with atorvastatin (30 mg kg(-1)) or vehicle (phosphate-buffered solution) once a day for three consecutive days impaired the increment in the production of IL-1β, PGE(2) and MMP-3 in the submandibular gland in the presence of anti-M(3) peptide IgG. In conclusion, the anti-inflammatory effects of atorvastatin are dependent upon inhibition of production of a pro-inflammatory cytokine (IL-1β) and pro-inflammatory mediators such as PGE(2) and MMP-3. These data suggest that atorvastatin may constitute an anti-inflammatory effect in SS.

show abstract

Autoantibodies against cerebral muscarinic cholinoceptors in Sjögren syndrome: functional and pathological implications

Cited by 31 publications

References 41 publications

Role of M<sub>3</sub> Muscarinic Acethylcholine Receptor Antibodies as a New Marker in Primary Sjögren Syndrome

Role of M<sub>3</sub> Muscarinic Acethylcholine Receptor Antibodies as a New Marker in Primary Sjögren Syndrome

Autoantibodies against Muscarinic Acetylcholine Receptor on Exocrine Glands in Sjögren Syndrome

Atorvastatin inhibits the inflammatory response caused by anti-M3 peptide IgG in patients with primary Sjögren’s syndrome

Contact Info

Product

Resources

About

Autoantibodies against cerebral muscarinic cholinoceptors in Sjögren syndrome: functional and pathological implications

Cited by 31 publications

References 41 publications

Role of M&lt;sub&gt;3&lt;/sub&gt; Muscarinic Acethylcholine Receptor Antibodies as a New Marker in Primary Sj&#246;gren Syndrome

Role of M&lt;sub&gt;3&lt;/sub&gt; Muscarinic Acethylcholine Receptor Antibodies as a New Marker in Primary Sj&#246;gren Syndrome

Autoantibodies against Muscarinic Acetylcholine Receptor on Exocrine Glands in Sjögren Syndrome

Atorvastatin inhibits the inflammatory response caused by anti-M3 peptide IgG in patients with primary Sjögren’s syndrome

Contact Info

Product

Resources

About

Role of M<sub>3</sub> Muscarinic Acethylcholine Receptor Antibodies as a New Marker in Primary Sjögren Syndrome

Role of M<sub>3</sub> Muscarinic Acethylcholine Receptor Antibodies as a New Marker in Primary Sjögren Syndrome