Autoantibody against arrestin-1 as a potential biomarker of renal cell carcinoma

Baldin, Alexey V.; Grishina, A. N.; Korolev, Dmitry; Kuznetsova, Ekaterina; Golovastova, Marina O.; Kalpinskiy, A. S.; Alekseev, B. Ya.; Каприн, А. Д.; Zinchenko, D. V.; Savvateeva, Lyudmila V.; Varshavsky, V A; Zernii, Evgeni Yu; Винаров, А.З.; Bazhin, Alexandr V.; Philippov, Pavel P.; Zamyatnin, Andrey A.

doi:10.1016/j.biochi.2018.10.019

Cited by 11 publications

(7 citation statements)

References 57 publications

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“…Thus, the identification of the tumor transcriptome and proteome to identify more immunogenic TSAs is helpful. Several highly immunogenic TSAs have already been described [260][261][262][263]. However, modern methods such as high-throughput sequencing technologies and bioinformatics analysis of big data allow the identification of new tumor antigenic determinants, such as neoantigens [264,265].…”

Section: Discussionmentioning

confidence: 99%

Dendritic Cells in Anticancer Vaccination: Rationale for Ex Vivo Loading or In Vivo Targeting

Baldin

Savvateeva

Bazhin

et al. 2020

Cancers

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Dendritic cells (DCs) have shown great potential as a component or target in the landscape of cancer immunotherapy. Different in vivo and ex vivo strategies of DC vaccine generation with different outcomes have been proposed. Numerous clinical trials have demonstrated their efficacy and safety in cancer patients. However, there is no consensus regarding which DC-based vaccine generation method is preferable. A problem of result comparison between trials in which different DC-loading or -targeting approaches have been applied remains. The employment of different DC generation and maturation methods, antigens and administration routes from trial to trial also limits the objective comparison of DC vaccines. In the present review, we discuss different methods of DC vaccine generation. We conclude that standardized trial designs, treatment settings and outcome assessment criteria will help to determine which DC vaccine generation approach should be applied in certain cancer cases. This will result in a reduction in alternatives in the selection of preferable DC-based vaccine tactics in patient. Moreover, it has become clear that the application of a DC vaccine alone is not sufficient and combination immunotherapy with recent advances, such as immune checkpoint inhibitors, should be employed to achieve a better clinical response and outcome.

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Section: Discussionmentioning

confidence: 99%

Dendritic Cells in Anticancer Vaccination: Rationale for Ex Vivo Loading or In Vivo Targeting

Baldin

Savvateeva

Bazhin

et al. 2020

Cancers

Self Cite

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“…Glutathione peroxidase assay kit was from Randox (Crumlin, UK). Polyclonal anti-visual arrestin antibodies were previously produced by animal immunization and affinity purification from hyperimmune serum [35]. Mouse monoclonal anti-visual arrestin antibodies were from Santa Cruz Biotechnology (Dallas, TX, USA) (sc-271159).…”

Section: Methodsmentioning

confidence: 99%

“…The content of disulfide dimers of visual arrestin in the retinal extracts was determined by non-reducing Western blotting as described in [20]. The arrestin was visualized using polyclonal (monospecific) antibodies obtained previously (1:10,000 in Tris buffer saline with 0.05% Tween-20 (TBST)) [35]. Alternatively, visual arrestin (C-1) mouse monoclonal IgG1 antibodies were used (1:5000 in TBST).…”

Section: Methodsmentioning

confidence: 99%

Suppression of Light-Induced Oxidative Stress in the Retina by Mitochondria-Targeted Antioxidant

et al. 2018

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Light-induced oxidation of lipids and proteins provokes retinal injuries and results in progression of degenerative retinal diseases, such as, for instance, iatrogenic photic maculopathies. Having accumulated over years retinal injuries contribute to development of age-related macular degeneration (AMD). Antioxidant treatment is regarded as a promising approach to protecting the retina from light damage and AMD. Here, we examine oxidative processes induced in rabbit retina by excessive light illumination with or without premedication using mitochondria-targeted antioxidant SkQ1 (10-(6’-plastoquinonyl)decyltriphenyl-phosphonium). The retinal extracts obtained from animals euthanized within 1–7 days post exposure were analyzed for H2O2, malondialdehyde (MDA), total antioxidant activity (AOA), and activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD) using colorimetric and luminescence assays. Oxidation of visual arrestin was monitored by immunoblotting. The light exposure induced lipid peroxidation and H2O2 accumulation in the retinal cells. Unexpectedly, it prominently upregulated AOA in retinal extracts although SOD and GPx activities were compromised. These alterations were accompanied by accumulation of disulfide dimers of arrestin revealing oxidative stress in the photoreceptors. Premedication of the eyes with SkQ1 accelerated normalization of H2O2 levels and redox-status of lipids and proteins, contemporarily enhancing AOA and, likely, sustaining normal activity of GPx. Thus, SkQ1 protects the retina from light-induced oxidative stress and could be employed to suppress oxidative damage of proteins and lipids contributing to AMD.

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“…In-depth research was conducted on SNP and methylation to find new courses for early detection of cancer [203][204][205][206]. The simultaneous detection of SNP and methylation at multiple sites by MNPs is conducive to the early diagnosis and prevention of cancer [207][208][209][210].…”

Section: Discussionmentioning

confidence: 99%

Application of magnetic nanoparticles in nucleic acid detection

Tang

et al. 2020

J Nanobiotechnol

202

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Nucleic acid is the main material for storing, copying, and transmitting genetic information. Gene sequencing is of great significance in DNA damage research, gene therapy, mutation analysis, bacterial infection, drug development, and clinical diagnosis. Gene detection has a wide range of applications, such as environmental, biomedical, pharmaceutical, agriculture and forensic medicine to name a few. Compared with Sanger sequencing, high-throughput sequencing technology has the advantages of larger output, high resolution, and low cost which greatly promotes the application of sequencing technology in life science research. Magnetic nanoparticles, as an important part of nanomaterials, have been widely used in various applications because of their good dispersion, high surface area, low cost, easy separation in buffer systems and signal detection. Based on the above, the application of magnetic nanoparticles in nucleic acid detection was reviewed.

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Autoantibody against arrestin-1 as a potential biomarker of renal cell carcinoma

Cited by 11 publications

References 57 publications

Dendritic Cells in Anticancer Vaccination: Rationale for Ex Vivo Loading or In Vivo Targeting

Dendritic Cells in Anticancer Vaccination: Rationale for Ex Vivo Loading or In Vivo Targeting

Suppression of Light-Induced Oxidative Stress in the Retina by Mitochondria-Targeted Antioxidant

Application of magnetic nanoparticles in nucleic acid detection

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