Autoantibody Biomarkers in Rheumatic Diseases

Kang, Eun Ha; Ha, You Jung; Lee, Yun Jong

doi:10.3390/ijms21041382

Cited by 32 publications

(39 citation statements)

References 176 publications

(239 reference statements)

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“…Our results showed that RF amplified after CFA injection at 10 and 21 but the level began to decline at 21 days without treatment; these findings were the same as other results [20,21], also an augmentation in MDA at 10 and 21 days of induction; this was in the accordance with Akyol et al, founded a remarkable elevation in MDA levels in patients with RA compared to controls [22], this was also observed by [23]; discussed that increased levels of S-nitrosothiols are present in RA plasma and knee-joint synovial fluid, which correlated with measures of inflammation in RA [24].…”

Section: Discussionsupporting

confidence: 92%

The Enhanced Beneficial Effect of Extra Virgin Olive Oil Over Evening Primrose Oil on Oxidative Stress, and Liver Function in Male Arthritic Rats

Kandeil

Ebrahim

Mahmoud

2021

JPRI

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Aims: Rheumatoid arthritis (RA) is characterized by the onset of oxidative stress. This study aimed to evaluate the enhancing of extra virgin olive (EVOO) and Evening primrose oil (EPO) on oxidative stress and liver enzymes in male Wistar rats and compare between them. Place and Duration: Faculty of Science biochemistry department, Between July 2018 and August 2018. Methodology: A Subcutaneous injection of 200 µl of Freund's complete adjuvant into a footpad of the right hind leg of Wistar male rats at two consecutive days induced RA. Rats received EVOO and EPO daily by oral gavage needle with gauge 18 at doses of 5 mg/kg b.wt./day. for 10 and 21 days. No loss was recorded in the experimental rats. Results: A significant depletion in serum Reduced glutathione content (GSH), glutathione peroxidase (GPX), and glutathione s transferase activities (GST) in arthritic rats compared to normal rats after 10 and 21 days of induction which improved significantly after 10 and 21 days of EPO and EVOO treatments. EPO and EVOO treatments for 21 days increased the GSH and GPX compared to 10 days treatments while no difference in GST activity. EVOO treatment improved GSH and GPX after 10 and 21 days than EPO treatment. The elevated uric acid levels in arthritic rats were markedly ameliorated as a result of EVOO and EPO treatment administration. Increased lipid peroxidation products (MDA), rheumatoid factor, and liver enzyme (Alanine transaminase ALT and Aspartate transaminase AST) were recorded in arthritic rats and they significantly progressed after EPO and EVOO treatments for 10 and 21 days but EVOO had the best effect at 21 days. Conclusion: EVOO and EPO showed significant antioxidant efficacies and improved affected liver enzymes due to rheumatoid arthritis onset. When comparing olive oil has more antioxidant properties than evening primrose oil, so we recommend more studies on olive oil combination with anti-arthritic medications to improve their efficacies with less toxicity.

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Section: Discussionsupporting

confidence: 92%

The Enhanced Beneficial Effect of Extra Virgin Olive Oil Over Evening Primrose Oil on Oxidative Stress, and Liver Function in Male Arthritic Rats

Kandeil

Ebrahim

Mahmoud

2021

JPRI

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“…Likewise, the sole consideration of healthy donors in control groups may lead to overestimated assay specificity, as the impact of potential cross-reactive factors present in the general population is largely ignored. In this respect, auto-antibodies commonly found in individuals with inflammatory diseases (22) were already described to cross-react with SARS-CoV-1 antigens (23). To challenge our tests systems, we biased our large specificity cohort (n=1,126) by including samples with an increased propensity for cross-reactivity, including sera from individuals with inflammatory illnesses (n=359), sera from PCR-confirmed hCoV infections (n=8) and sera drawn during winter months to increase the likelihood of respiratory infections (n=494).…”

Section: Discussionmentioning

confidence: 99%

A comprehensive antigen production and characterization study for easy-to-implement, highly specific and quantitative SARS-CoV-2 antibody assays

Klausberger

Duerkop

Haslacher

et al. 2021

Preprint

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Antibody tests are essential tools to investigate humoral immunity following SARS-CoV-2 infection. While first-generation antibody tests have primarily provided qualitative results with low specificity, accurate seroprevalence studies and tracking of antibody levels over time require highly specific, sensitive and quantitative test setups. Here, we describe two quantitative ELISA antibody tests based on the SARS-CoV-2 spike receptor-binding domain and the nucleocapsid protein. Comparative expression in bacterial, insect, mammalian and plant-based platforms enabled the identification of new antigen designs with superior quality and high suitability as diagnostic reagents. Both tests scored excellently in clinical validations with multi-centric specificity and sensitivity cohorts and showed unprecedented correlation with SARS-CoV-2 neutralization titers. Orthogonal testing increased assay specificity to 99.8%, thereby enabling robust serodiagnosis in low-prevalence settings. The inclusion of a calibrator permits accurate quantitative monitoring of antibody concentrations in samples collected at different time points during the acute and convalescent phase of COVID-19.

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“…Likewise, the sole consideration of healthy donors in control groups may lead to overestimated assay specificity, as the impact of potential cross-reactive factors present in the general population is largely ignored. In this respect, auto-antibodies commonly found in individuals with inflammatory diseases [58] were already described to cross-react with SARS-CoV-1 antigens [59] . To challenge our tests systems, we biased our large specificity cohort ( n = 1126) by including samples with an increased propensity for cross-reactivity, including sera from individuals with inflammatory illnesses ( n = 359), sera from PCR-confirmed hCoV infections ( n = 8) and sera drawn during winter months to increase the likelihood of respiratory infections ( n = 494).…”

Section: Discussionmentioning

confidence: 99%

A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests

Klausberger¹,

Duerkop²,

Haslacher³

et al. 2021

EBioMedicine

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Background: Antibody tests are essential tools to investigate humoral immunity following SARS-CoV-2 infection or vaccination. While first-generation antibody tests have primarily provided qualitative results, accurate seroprevalence studies and tracking of antibody levels over time require highly specific, sensitive and quantitative test setups. Methods: We have developed two quantitative, easy-to-implement SARS-CoV-2 antibody tests, based on the spike receptor binding domain and the nucleocapsid protein. Comprehensive evaluation of antigens from several biotechnological platforms enabled the identification of superior antigen designs for reliable serodiagnostic. Cut-off modelling based on unprecedented large and heterogeneous multicentric validation cohorts allowed us to define optimal thresholds for the tests' broad applications in different aspects of clinical use, such as seroprevalence studies and convalescent plasma donor qualification. Findings: Both developed serotests individually performed similarly-well as fully-automated CE-marked test systems. Our described sensitivity-improved orthogonal test approach assures highest specificity (99.8%); thereby enabling robust serodiagnosis in low-prevalence settings with simple test formats. The inclusion of a calibrator permits accurate quantitative monitoring of antibody concentrations in samples collected at different time points during the acute and convalescent phase of COVID-19 and disclosed antibody level thresholds that correlate well with robust neutralization of authentic SARS-CoV-2 virus. Interpretation: We demonstrate that antigen source and purity strongly impact serotest performance. Comprehensive biotechnology-assisted selection of antigens and in-depth characterisation of the assays allowed us to overcome limitations of simple ELISA-based antibody test formats based on chromometric reporters, to yield comparable assay performance as fully-automated platforms.

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Autoantibody Biomarkers in Rheumatic Diseases

Cited by 32 publications

References 176 publications

The Enhanced Beneficial Effect of Extra Virgin Olive Oil Over Evening Primrose Oil on Oxidative Stress, and Liver Function in Male Arthritic Rats

The Enhanced Beneficial Effect of Extra Virgin Olive Oil Over Evening Primrose Oil on Oxidative Stress, and Liver Function in Male Arthritic Rats

A comprehensive antigen production and characterization study for easy-to-implement, highly specific and quantitative SARS-CoV-2 antibody assays

A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests

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