Autoantigens in ovarian autoimmunity associated with unexplained infertility and premature ovarian failure

Abstract

Study Objective-To identify ovarian autoantigens associated with ovarian autoantibodies.Design-Hypothesis generating prospective study. Setting-Urban infertility referral centers and academic research institution.Patients-74 patients with infertility, 19 patients with premature ovarian failure, 16 healthy control women. Interventions-None. Main outcome measures-Identification of autoantigens.Results-In order to identify major antigens for ovarian autoimmunity, 74 sera from women with unexplained infertility were screened for ovarian autoantibodies (AOA) by immunoassay and one-dimensional Western blot. The majority of sera had immuno-reactions at 50-56kDa. Six representative positive infertility sera were used to identify antigens between 40-60kD by twodimensional Western blot and mass spectrometry. Antigens included aldehyde (retinal) dehydrogenases (ALDH1A1, ALDH1A2, ALDH7A1), protein disulfide-isomerase A3 (PDIA3), vimentin (VIME), α-enolase (ENO1), phosphoglycerate dehydrogenase and selenium binding protein 1 (SBP1). 60% (n=24/40) of infertility and POF sera were positive for recombinant ALDH1A1, SBP1 or enolase. 80.7% (n=21/26) of AOA positive sera had antibodies to one or Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript more of the three antigens, while only 7% (n=1/14) of AOA negative sera had antibodies to recombinant proteins. Conclusion-ALDH1A1and SBP1 are unique to ovarian autoimmunity associated with infertility and POF, and may provide the basis for specific tests to identify patients with ovarian autoimmunity.

Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

“…Previous reports have identified PDIA3 by MS in the mammalian ovary tissue and [65] in embryo culture media [66]. Its function in the reproductive systems has not yet been elucidated; however, it could be suggested that it includes cell growth and death.…”

Determination of Complementary and Alternative Medicine Practices Used by Women Applying to Infertility Clinic

“…19 Prematür ovaryan yetmezliği ya da açıklanamayan infertilitesi olanlarda selenyum bağlayıcı protein-1'e karşı otoantikorlar saptanmıştır. 20 Melatonin bir başka antioksidan madde olup, ortamdaki serbest radikalleri temizleyerek antioksidan enzimleri indüklemektedir. Kontrollü ovaryan stimülasyon sırasında melatonin takviyesinin oositi oksidatif stresten koruyup IVF sonuçlarını iyileştirmesi beklenebilir.…”

Ovarian Biomarkers in Infertility

“…It has been demonstrated that 21 of 110 women with autoimmune POF express this autoantibody, whereas 60 control subjects proved negative. Aldehyde (retinal) dehydrogenase 1A1 (ALDH1A1) and selenium-binding protein 1 (SBP1) are identified as unique autoantigenic targets associated with unexplained infertility (Edassery et al, 2010).…”