Autochthonous canine visceral leishmaniasis cases occur in Paraná state since 2012: isolation and identification of Leishmania infantum

Abstract

The aim of this study was to confirm the emergence of canine visceral leishmaniasis among dogs in Foz do Iguaçu. The disease was diagnosed through the isolation and molecular identification of Leishmania infantum. In the first sample collection stage (2012), three lymph node aspirates and 46 buffy coat samples were obtained mostly from the dogs that were seroreagents for leishmaniasis. In the second sample collection stage (2013), the buffy coat samples were collected from 376 dogs located close to Paraguay, Paraná river, center and peripheral parts of the city. The DNA from the six isolates, four from the first sampling stage (4/49) and two from the second sampling stage (2/376), was subjected to polymerase chain reaction using the K26F/R primers. The isolate was confirmed as L. infantum by sequencing. As none of the dogs had ever left the city, the isolates were confirmed as autochthonous. Further, the study confirmed the emergence of canine visceral leishmaniasis in Paraná through the identification of L. infantum among dogs in Foz do Iguaçu city. Hence, collaborative control measures should be designed and implemented by the public agencies and research institutions of Brazil, Argentina, and Paraguay to control the spread of visceral leishmaniasis.

Hiperadrenocorticismo Canino E Felinorevisão De Literatura

“…Contaminação em isolamento primário e manutenção de culturas de células é um problema grave e frequente. Os principais antibacterianos utilizados são penicilina, estreptomicina e gentamicina usados de forma isolada ou associada (LOCATELLI et al, 2014;KALANTARI et al, 2018;SANTOS et al, 2018;DIAS et al, 2020;). Contudo, a prevenção de contaminação fúngica no isolamento e manutenção de culturas de Leishmania spp.…”

Achados Radiográficos Torácicos De Cães E Gatos Atendidos Em Uma Clínica Veterinária Na Cidade De Teresina – Pi

“…A contaminação ocorrida em 20% das amostras analisadas em nosso estudo está de acordo com outros achados na literatura (SAMPAIO et al, 2002;OLIVEIRA et al, 2010;SANTOS et al, 2018 Contaminação em isolamento primário e manutenção de culturas de células é um problema grave e frequente. Os principais antibacterianos utilizados são penicilina, estreptomicina e gentamicina usados de forma isolada ou associada (LOCATELLI et al, 2014;KALANTARI et al, 2018;SANTOS et al, 2018;DIAS et al, 2020;). Contudo, a prevenção de contaminação fúngica no isolamento e manutenção de culturas de Leishmania spp.…”

First case of canine visceral leishmaniasis in the midwestern of Santa Catarina State, Brazil

“…So far, autochthonous canine visceral leishmaniasis (CVL) transmission was reported in the southern Brazilian states of Rio Grande do Sul, Santa Catarina, and Paraná (Deboni et al, 2011;Steindel et al, 2013;Maziero et al, 2014;Pasquali et al, 2019;Dias et al, 2020). In Santa Catarina state, CVL was originally reported in the eastern coastal city of Florianopolis in 2010 and, according to the local Center for Zoonotic Diseases Control (CCZ), since then a total of 481 CVL cases were confirmed among 14,190 dogs tested (Florianópolis, 2020).…”