Autochthonous lactic acid bacteria—presentation of potential probiotics application

Grujović, Mirjana Ž.; Mladenović, Katarina G.; Nikodijević, Danijela D.; Čomić, Ljiljana R.

doi:10.1007/s10529-019-02729-8

Cited by 19 publications

(10 citation statements)

References 30 publications

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“…The ability to survive the sequential stresses encountered during passage through the human digestive tract is a relevant feature of probiotic LAB (van Bokhorst-van de Veen et al 2012), and OGI transit assays are helpful to predict the robustness of probiotics, in view of their use as components of functional foods (Grujović et al 2019;Fiocco et al 2020). The extreme acidity encountered in the stomach usually constitutes a major pitfall for orally-ingested, foodborne bacteria, and this was previously demonstrated also for L. plantarum, and other probiotic LAB, by using different OGI systems (Bove et al 2012;van Bokhorst-van de Veen et al 2012;Arena et al 2014;Arena et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Knock out of sHSP genes determines some modifications in the probiotic attitude of Lactiplantibacillus plantarum

et al. 2020

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Objective We investigated whether the knock out of small heat shock protein (sHSP) genes (hsp1, hsp2 and hsp3) impact on probiotic features of Lactiplantibacillus plantarum WCFS1, aiming to find specific microbial effectors involved in microbe-host interplay. Results The probiotic properties of L. plantarum WCFS1 wild type, hsp1, hsp2 and hsp3 mutant clones were evaluated and compared through in vitro trials. Oro-gastro-intestinal assays pointed to significantly lower survival for hsp1 and hsp2 mutants under stomach-like conditions, and for hsp3 mutant under intestinal stress. Adhesion to human enterocyte-like cells was similar for all clones, though the hsp2 mutant exhibited higher adhesiveness. L. plantarum cells attenuated the transcriptional induction of pro-inflammatory cytokines on lipopolysaccharide-treated human macrophages, with some exception for the hsp1 mutant. Intriguingly, this clone also induced a higher IL10/IL12 ratio, which is assumed to indicate the anti-inflammatory potential of probiotics. Conclusions sHSP genes deletion determined some differences in gut stress resistance, cellular adhesion and immuno-modulation, also implying effects on in vivo interaction with the host. HSP1 might contribute to immunomodulatory mechanisms, though additional experiments are necessary to test this feature.

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Section: Discussionmentioning

confidence: 99%

Knock out of sHSP genes determines some modifications in the probiotic attitude of Lactiplantibacillus plantarum

et al. 2020

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“…Grujović et al. (2019) have also reported an acid tolerance (pH 3.0) behavior of two novel probiotic bacteria Lb. fermentum KGPMF28 and KGPMF29 up to 70.1% and 72.6%, respectively.…”

Section: Environmental Stressesmentioning

confidence: 95%

“…Grujović et al. (2019) have reported the capability of Lactobacillus fermentum (KGPMF28 and KGPMF2) to grow at 45 °C for 24 hr. A study on the molecular mechanisms underlying LAB adaptations and survival at high temperatures is, therefore, important for selecting LAB species as starter cultures and probiotics.…”

Section: Environmental Stressesmentioning

confidence: 99%

“…fermentum KGPMF28 and KGPMF29 up to 70.1% and 72.6%, respectively. The acid tolerance rose to 87.6% in the case of Lactobacillus plantarum LP 299v (Grujović et al., 2019). The responses of various probiotics against acid stress are listed in Table 3.…”

Section: Environmental Stressesmentioning

confidence: 99%

“…A clear understanding of how LAB react to environmental stresses is greatly important for the identification and selection of strains with enhanced performance as starter cultures and probiotics (Abhisingha, Dumnil, & Pitaksutheepong, 2018; Dowarah, Verma, Agarwal, Singh, & Singh, 2018; Kim, Shin, Lee, Moon, & Lee, 2018). It is important to thoroughly understand the conditions that support LAB survival and the system that allows them to survive under severe conditions (Grujović, Mladenović, Nikodijević, & Čomić, 2019; Papadimitriou et al., 2016). This review discusses the mechanisms and adaptations underlying probiotic LAB survival under different environmental stress conditions.…”

Section: Introductionmentioning

confidence: 99%

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Updates on understanding of probiotic lactic acid bacteria responses to environmental stresses and highlights on proteomic analyses

Mbye

Baig

AbuQamar

et al. 2020

Comp Rev Food Sci Food Safe

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Probiotics are defined as live microorganisms that improve the health of the host when administered in adequate quantities. Nonetheless, probiotics encounter extreme environmental conditions during food processing or along the gastrointestinal tract. This review discusses different environmental stresses that affect probiotics during food preparation, storage, and along the alimentary canal, including high temperature, low temperature, low and alkaline pH, oxidative stress, high hydrostatic pressure, osmotic pressure, and starvation. The understanding of how probiotics deal with environmental stress and thrive provides useful information to guide the selection of the strains with enhanced performance in specific situations, in food processing or during gastrointestinal transit. In most cases, multiple biological functions are affected upon exposure of the cell to environmental stress. Sensing of sublethal environmental stress can allow for adaptation processes to occur, which can include alterations in the expression of specific proteins.

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Screening of lactic acid bacteria from the milk of Sahiwal cows and characterization of their probiotic potential for preventing bovine mastitis

Chauhan,

Kumar,

Chauhan

et al. 2024

Int Microbiol

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Autochthonous lactic acid bacteria—presentation of potential probiotics application

Cited by 19 publications

References 30 publications

Knock out of sHSP genes determines some modifications in the probiotic attitude of Lactiplantibacillus plantarum

Knock out of sHSP genes determines some modifications in the probiotic attitude of Lactiplantibacillus plantarum

Updates on understanding of probiotic lactic acid bacteria responses to environmental stresses and highlights on proteomic analyses

Screening of lactic acid bacteria from the milk of Sahiwal cows and characterization of their probiotic potential for preventing bovine mastitis

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