Autocidal control of ticks by silencing of a single gene by RNA interference

Fuente, José de la; Almazán, Consuelo; Naranjo, Victoria; Blouin, Edmour F.; Meyer, John M.; Kocan, Katherine M.

doi:10.1016/j.bbrc.2006.03.109

Cited by 64 publications

(56 citation statements)

References 20 publications

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“…Recent developments, including transgenic vectors (Christophides 2005;Sperança and Capurro 2007), RNA interference (RNAi; Blair et al 2006;Brown and Catteruccia 2006;de la Fuente et al 2006a), and pathogen-derived antigens for development of transmission-blocking vaccines Kedzierski et al 2006;Saul 2007), have shown to be a promising alternative for the control of ticks and tick-borne pathogens. However, a limited number of vaccines against arthropods resulting in generation of antigen-specific antibodies and injury to the arthropod during feeding have been reported (de la Fuente et al 1998(de la Fuente et al , 2007aValenzuela et al 2001;Lal et al 2001;Almeida and Billingsley 2002;Suneja et al 2003;de la Fuente and Kocan 2003;Willadsen 2004;Milleron et al 2004;Titus et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Conservation and immunogenicity of the mosquito ortholog of the tick-protective antigen, subolesin

et al. 2009

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The control of arthropod vectors of pathogens that affect human and animal health is important for the eradication of vector-borne diseases. The ortholog of the tick-protective antigen, subolesin, was identified in Aedes albopictus and found to have conserved epitopes in ticks and mosquitoes. RNA interference with the tick and mosquito double-stranded RNA in three tick species resulted in significant gene knockdown and decreased tick weight and/or survival. Feeding Anopheles atroparvus, Aedes caspius, and Culex pipiens female mosquitoes on an A. albopictus subolesin hyperimmune serum resulted in 11 +/- 5% to 29 +/- 6% survival inhibition when compared to controls fed on preimmune serum. Feeding sand flies, Phlebotomus perniciosus, on antimosquito subolesin ortholog protein antibodies inhibited female survival and the number of larvae and adults obtained after hatching by 28 +/- 22% and 16 +/- 3%, respectively, when compared to controls. Vaccination with tick and mosquito subolesin ortholog proteins significantly reduced Ixodes scapularis tick infestation and weight in a similar way. However, vaccination with the recombinant mosquito subolesin ortholog antigen did not protect against Amblyomma americanum and Rhipicephalus sanguineus tick infestations. Collectively, these preliminary results provided the first evidence that development of vaccines may be possible for control of multiple arthropod vectors using subolesin orthologs but suggested that multiple antigens may be required to produce an effective vaccine.

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Section: Introductionmentioning

confidence: 99%

Conservation and immunogenicity of the mosquito ortholog of the tick-protective antigen, subolesin

et al. 2009

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“…Quite similarly, the application of RNAi for tick control was also proposed in a single report on D. variabilis, wherein the highly conserved subolesin was targeted leading to reproductive incapacity [38]. In conclusion, the authors suggested that RNAi may be used to massively produce sterile ticks (SAT) that may be released in the field.…”

Section: Future Directions In Tick Research and Application In Tick Cmentioning

confidence: 95%

“…In most reports, a high concentration of at least 1 µg dsRNA per tick has been shown to be effective in inducing gene silencing [33], but in some reports, lower concentration has been found to be similarly effective [34][35][36]. Injection has been accomplished using a 33-36-gauge needle attached to a Hamilton syringe particularly in large tick species, such as Amblyomma americanum [37], Dermacentor variabilis, and D. marginatus [38], while microinjection using a microcapillary drawn to a fine point needle and inserted to a micromanipulator has been commonly employed in smaller tick species, including Ixodes [39], and Haemaphysalis [26] ticks. Different injection sites include the lower right quadrant of the ventral surface of the exoskeleton [40], the groove between the basis capituli and the scutum [37], the ventral torso of the idiosoma, away from the anal opening [39], and the coxal membrane in the fourth coxae [26,41].…”

Section: Injectionmentioning

confidence: 99%

“…The function of subolesin is unclear, but a report showed that subolesin knockdown affected the expression of several genes involved in multiple cellular pathways, suggesting a role in gene expression by interacting with regulatory proteins [154]. Aside from being reported as a promising anti-tick vaccine antigen candidate in many studies, it has been also proposed that subolesin may be targeted in ticks that subsequently will be released for sterile acarine technique (SAT) [38].…”

Section: Rnai Studies On Tick Protective Antigens and Immunitymentioning

confidence: 99%

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RNA Interference – A Powerful Functional Analysis Tool for Studying Tick Biology and its Control

Galay¹,

Umemiya‐Shirafuji²,

MasamiMochizuki³

et al. 2016

RNA Interference

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Ticks (Acari: Ixodida) are blood-sucking arthropods globally recognized as vectors of numerous diseases. They are primarily responsible for the transmission of various pathogens, including viruses, rickettsiae, and blood parasites of animals. Ticks are second to mosquitoes in terms of disease transmission to humans. The continuous emergence of tick-borne diseases and acaricide resistance of ticks necessitates the development of new and more effective control agents and strategies; therefore, understanding of different aspects of tick biology and their interaction with pathogens is very crucial in developing effective control strategies. RNA interference (RNAi) has been widely used in the area of tick research as a versatile reverse genetic tool to elucidate the functions of various tick proteins. During the past decade, numerous studies on ticks utilized RNAi to evaluate potentially key tick proteins involved in blood feeding, reproduction, evasion of host immune response, interaction with pathogens, and pathogen transmission that may be targeted for tick and pathogen control. This chapter reviewed the application of RNAi in tick research over the past decade, focusing on the impact of this technique in the advancement of knowledge on tick and pathogen biology.

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“…RNAi inhibition of subolesin resulted in sterile wood ticks (Dermacentor variabilis) with under-developed salivary glands (de la Fuente et al 2006b). Subsequently, mice vaccinated with subolesin were shown to have a 3-fold reduction in the number of I. scapularis nymphs infected with A. phagocytophilum.…”

Section: Combating Infectious Diseasementioning

confidence: 99%

RNA interference-based technology: what role in animal agriculture?

et al. 2017

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Abstract. Animal agriculture faces a broad array of challenges, ranging from disease threats to adverse environmental conditions, while attempting to increase productivity using fewer resources. RNA interference (RNAi) is a biological phenomenon with the potential to provide novel solutions to some of these challenges. Discovered just 20 years ago, the mechanisms underlying RNAi are now well described in plants and animals. Intracellular double-stranded RNA triggers a conserved response that leads to cleavage and degradation of complementary mRNA strands, thereby preventing production of the corresponding protein product. RNAi can be naturally induced by expression of endogenous microRNA, which are critical in the regulation of protein synthesis, providing a mechanism for rapid adaptation of physiological function. This endogenous pathway can be co-opted for targeted RNAi either through delivery of exogenous small interfering RNA (siRNA) into target cells or by transgenic expression of short hairpin RNA (shRNA). Potentially valuable RNAi targets for livestock include endogenous genes such as developmental regulators, transcripts involved in adaptations to new physiological states, immune response mediators, and also exogenous genes such as those encoded by viruses. RNAi approaches have shown promise in cell culture and rodent models as well as some livestock studies, but technical and market barriers still need to be addressed before commercial applications of RNAi in animal agriculture can be realised. Key challenges for exogenous delivery of siRNA include appropriate formulation for physical delivery, internal transport and eventual cellular uptake of the siRNA; additionally, rigorous safety and residue studies in target species will be necessary for siRNA delivery nanoparticles currently under evaluation. However, genomic incorporation of shRNA can overcome these issues, but optimal promoters to drive shRNA expression are needed, and genetic engineering may attract more resistance from consumers than the use of exogenous siRNA. Despite these hurdles, the convergence of greater understanding of RNAi mechanisms, detailed descriptions of regulatory processes in animal development and disease, and breakthroughs in synthetic chemistry and genome engineering has created exciting possibilities for using RNAi to enhance the sustainability of animal agriculture.

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Autocidal control of ticks by silencing of a single gene by RNA interference

Cited by 64 publications

References 20 publications

Conservation and immunogenicity of the mosquito ortholog of the tick-protective antigen, subolesin

Conservation and immunogenicity of the mosquito ortholog of the tick-protective antigen, subolesin

RNA Interference – A Powerful Functional Analysis Tool for Studying Tick Biology and its Control

RNA interference-based technology: what role in animal agriculture?

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