Autocrine growth inhibition of a cloned line of helper T cells.

Horowitz, Jay B.; Kaye, Jonathan; Conrad, Patricia J.; Katz, Michael E.; Janeway, Charles A.

doi:10.1073/pnas.83.6.1886

Cited by 37 publications

(20 citation statements)

References 19 publications

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“…On the other hand, Th2 cells produce IL-4 which stimulates the IgE antibody response, IL-5 which stimulates eosinophils, and IL-10 which together with IL-4 suppresses cellmediated immunity. There is a mutually excluding feedback system where cytokines produced by one of the Th1 and Th2 subsets inhibit development of the other [5,6]. Although the demarcation of CD4 T cells in humans is not as sharp as it is in mice, accumulating evidence supports this classification [7].…”

Section: Introductionmentioning

confidence: 97%

Differential Effects of Neuropeptides on Cytokine Production by Mouse Helper T Cell Subsets

Kawamura

Tamura²,

Obana³

et al. 1998

Neuroimmunomodulation

109

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Though immune outcome is known to be determined by which helper T cell response predominates, no local mechanism has yet been established which can explain how the neuronal system may control this. It is possible that the nervous system releases neuropeptides at specific local sites of infection or challenge, which triggers lymphocytes at those points to release specific cytokine profiles. These may then influence the direction of the Th1/Th2 response and therefore immune outcome. The aim of this study was to evaluate whether and if so how neuropeptides influence cytokine production by lymphocytes, especially T cells. We investigated the effects of neuropeptide Y (NPY), calcitonin gene-related peptide (CGRP), substance P (SP) and vasoactive intestinal peptide (VIP) on the production of interferon-γ (IFN-γ) and interleukin-4 (IL-4) by stimulating nonadherent splenocytes and helper T cell clones with antigens in vitro in the presence or absence of these peptides. NPY greatly enhanced IL-4 production and inhibited IFN-γ. CGRP inhibited IFN-γ production markedly in a dose-dependent manner, but had no effects on IL-4 production. SP and VIP had no effects on IFN-γ production, but SP enhanced and VIP suppressed IL-4 production slightly but consistently. Therefore neuropeptides can influence cytokine production. This opens the door to speculations that these specific cytokine profiles might play a part in influencing the direction of the consequent Th1/Th2 cascade and immune outcome and possibly the pathogenesis of immune-related diseases.

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Section: Introductionmentioning

confidence: 97%

Differential Effects of Neuropeptides on Cytokine Production by Mouse Helper T Cell Subsets

Kawamura

Tamura²,

Obana³

et al. 1998

Neuroimmunomodulation

109

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“…To do this, we stably transfected CIITA to three T cell lines, the 68-41 Th1 hybridoma (22), the 002 T cell hybridoma (23), and the D10 Th2 cell clone (24). As shown previously (21,23), T cells transfected with CIITA expressed MHC class II ( Fig.…”

Section: Ciita Inhibits Induction Of Endogenous Fas Ligand Expressionmentioning

confidence: 99%

“…The 68-41 Th1 cell hybridoma (22), the 002 T cell hybridoma (23), the D10 Th2 clone (24), the Jurkat T cell line, and the primary cells isolated from C57BL/6 mice were all maintained in Click's medium supplemented with 10% FBS, 2 mM glutamine, 100 g/ml penicillin and streptomycin, and 10 Ϫ5 M 2-ME. The A20 mouse B cell line was maintained in RPMI 1640 medium with the same supplements as above.…”

Section: Cell Lines and Micementioning

confidence: 99%

Cutting Edge: The Class II Transactivator Prevents Activation-Induced Cell Death by Inhibiting Fas Ligand Gene Expression

Gourley

Chang

2001

The Journal of Immunology

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The Fas:Fas ligand pathway is critical in regulating immune homeostasis by eliminating activated T cells that proliferated in response to an infection. Here, we show that the MHC class II transactivator (CIITA) can suppress this pathway by inhibiting transcription of the Fas ligand gene. CIITA can effectively repress transcription from the Fas ligand promoter in both T cell lines as well as primary cells. The repression appears to be at least partly due to interference of NFAT-mediated induction of Fas ligand gene transcription. T cells that express CIITA constitutively do not up-regulate Fas ligand on the cell surface after activation via the TCR. Consequently, these cells lack the ability to undergo activation-induced cell death, and to kill Fas-bearing target cells.

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“…Thl helper T-cell clones secrete interleukin 2 (IL-2) and interferon y (IFN-y) and preferentially induce macrophage activation and DTH (10,11), while Th2 clones produce IL-4 and IL-5 (12,13) and provide superior help for humoral responses (14)(15)(16)(17). If the functions of Thl and Th2 clones reflect the different classes of immune response, then these cells may be mutually inhibitory (18)(19)(20). Cytokine synthesis inhibitory factor (CSIF; IL-10), a product ofmurine Th2 clones, inhibits cytokine secretion by Thl cells (21).…”

mentioning

confidence: 99%

Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI.

Vieira

Malefyt

Dang

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

676

363

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We have demonstrated the existence of human cytokine synthesis inhibitory factor (CSIF) [interleukin 10 (IL-10)]. cDNA clones encoding human IL-10 (hIL-10) were isolated from a tetanus toxin-specific human T-cell clone. Like mouse IL-10, hIL-10 exhibits strong DNA and amino acid sequence homology to an open reading frame in the EpsteinBarr virus, BCRFI. hIL-lO and the BCRFI product inhibit cytokine synthesis by activated human peripheral blood mononuclear cells and by a mouse Thl clone. Both hIL-10 and mouse IL-1O sustain the viability of a mouse mast cell line in culture, but BCRFI lacks comparable activity in this assay, suggesting that BCRFI may have conserved only a subset of hIL-10 activities.

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Autocrine growth inhibition of a cloned line of helper T cells.

Cited by 37 publications

References 19 publications

Differential Effects of Neuropeptides on Cytokine Production by Mouse Helper T Cell Subsets

Differential Effects of Neuropeptides on Cytokine Production by Mouse Helper T Cell Subsets

Cutting Edge: The Class II Transactivator Prevents Activation-Induced Cell Death by Inhibiting Fas Ligand Gene Expression

Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI.

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