Currently, the workload and strict schedules lead employees to neglect their physical and mental health, which leads them to have a more sedentary life, which can become a risk factor for work-related diseases and mortality. The objective of this research is to determine the level of sedentary lifestyle by analyzing the variables associated with this behavior, in administrative and academic workers of a higher education institution in Mexico. The research followed a descriptive design and correlational, applying the Deming cycle methodology, for which information was collected through a direct survey and data analysis with a statistical program, on physical activity and sedentary lifestyle, in the in which a total of 108 respondents participated. A high prevalence of sedentary lifestyle was observed, present in 66.6% of the population studied and it was verified that 47,2% of the workers did not comply with the minimum recommendations for physical activity. The study revealed a high prevalence of sedentary behavior in workers. In addition, a dependency relationship of sedentary lifestyle with physical activity, gender and glucose levels was identified, these being the variables that explain this behavior in a significant way.