In recent decades, multispectral imaging (MSI) has been approved as an effective tool to detect and discriminate targets in industrial application. A narrowband micro-arrayed multispectral filter mosaic (MSFM) based on MSI technology is proposed to miniaturize MSI instrumentation. There are many reasons to believe that this innovation is going to replace the traditional methods of filtering or dispersing light and bring MSI applications to more industrial fields. However, highly integrated micro pieces of MSFM are arranged periodically, and the spectral profiles of two adjacent pieces cause a spectral interference (SI). To express and estimate the extent of SI, an evaluation method is proposed and its feasibility is reported in this paper. Additionally, the ways reducing the extent of SI are suggested.