Autohydrogenotrophic Denitrifying Microbial Community in a Glass Beads Biofilm Reactor

Park, Ho Il; Choi, Yong-Jin; Pak, Daewon

doi:10.1007/s10529-005-7654-x

Cited by 61 publications

(29 citation statements)

References 9 publications

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“…The primer pair AM1/NS31 did not amplify 18S rDNA fragments from all known AMF ) and could amplify some non-AMF sequences (Douhan et al 2005;Ma et al 2005). But no other primer pair had been used in DGGE analysis (Liang et (Park et al 2008;Maukonen et al 2006;Federici et al 2007). But in some researches, one step PCR was used to get the objective fragments (Docherty et al 2006;Wang et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Communities of arbuscular mycorrhizal fungi and bacteria in the rhizosphere of Caragana korshinkii and Hippophae rhamnoides in Zhifanggou watershed

et al. 2009

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The process of ecological restoration and reconstruction in Zhifanggou watershed forms a special ecosystem on the Loess Plateau. Little is known about the communities of arbuscular mycorrhizal fungi (AMF) and bacteria in this ecosystem. The aim of this study was to analyze the communities of AMF and bacteria, and their relationship in the rhizosphere of Caragana korshinkii and Hippophae rhamnoides in Zhifanggou watershed. Soil samples were collected from Zhifanggou watershed. The communities of AMF and bacteria were analyzed by using nested PCR of rDNA fragments and denaturing gradient gel electrophoresis (DGGE). Diversity analysis revealed that the bacterial Shannon diversity index was higher than that of AMF, and the AMF and bacterial Shannon diversity index in the rhizosphere of H. rhamnoides was higher than that of C. korshinkii. Principal component analysis (PCA) revealed that host plant had a significant influence on the bacterial community structure, but no strict specificity with AMF. Correlation analysis showed that the AMF communities had a significant positive correlation with the bacterial communities, and that indicated a significant effect of AMF on bacteria.

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Section: Discussionmentioning

confidence: 99%

Communities of arbuscular mycorrhizal fungi and bacteria in the rhizosphere of Caragana korshinkii and Hippophae rhamnoides in Zhifanggou watershed

et al. 2009

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“…Likewise, a 16S rRNA gene DGGE community analysis of denitrifying biofilm reactor communities with hydrogen as an electron donor also showed the significant dominance of Clostridium spp. (Park et al, 2005). Noted that Gram-positive bacteria were enriched in denitrifying biocathode communities and were not-detected in control cathodes without current.…”

Section: Identification Of Bacteria Most Active In Denitrifying Biofilmsmentioning

confidence: 99%

Bacterial community structure corresponds to performance during cathodic nitrate reduction

et al. 2010

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Microbial fuel cells (MFCs) have applications other than electricity production, including the capacity to power desirable reactions in the cathode chamber. However, current knowledge of the microbial ecology and physiology of biocathodes is minimal, and as a result more research dedicated to understanding the microbial communities active in cathode biofilms is required. Here we characterize the microbiology of denitrifying bacterial communities stimulated by reducing equivalents generated from the anodic oxidation of acetate. We analyzed biofilms isolated from two types of cathodic denitrification systems: (1) a loop format where the effluent from the carbon oxidation step in the anode is subjected to a nitrifying reactor which is fed to the cathode chamber and (2) an alternative non-loop format where anodic and cathodic feed streams are separated. The results of our study indicate the superior performance of the loop reactor in terms of enhanced current production and nitrate removal rates. We hypothesized that phylogenetic or structural features of the microbial communities could explain the increased performance of the loop reactor. We used PhyloChip with 16S rRNA (cDNA) and fluorescent in situ hybridization to characterize the active bacterial communities. Our study results reveal a greater richness, as well as an increased phylogenetic diversity, active in denitrifying biofilms than was previously identified in cathodic systems. Specifically, we identified Proteobacteria, Firmicutes and Chloroflexi members that were dominant in denitrifying cathodes. In addition, our study results indicate that it is the structural component, in terms of bacterial richness and evenness, rather than the phylogenetic affiliation of dominant bacteria, that best corresponds to cathode performance.

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“…and Hydrogenophaga sp. [1] and Rhodocyclus and Hydrogenophaga [82] were isolated from mixed microbial communities of hydrogenotrophic reactors. Bacterial communities within hydrogenotrophic denitrifying biofilms that belonged to the classes of Flavobacteria [81] and Sphingobacteria [80] have also been reported.…”

Section: Microbiologymentioning

confidence: 99%

“…Worldwide an increase of nitrate concentrations observed in groundwater, as a result of the use of fertilizers, and the industrial wastewater, raises concerns due to the severe impacts on human health [1]. Research is carried out towards nitrate removal from water resources, whereas the most promising approach being studied is biological denitrification.…”

Section: Introductionmentioning

confidence: 99%

Hydrogenotrophic denitrification of potable water: A review

Καρανάσιος

Vasiliadou

Pavlou

et al. 2010

Journal of Hazardous Materials

323

143

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Autohydrogenotrophic Denitrifying Microbial Community in a Glass Beads Biofilm Reactor

Cited by 61 publications

References 9 publications

Communities of arbuscular mycorrhizal fungi and bacteria in the rhizosphere of Caragana korshinkii and Hippophae rhamnoides in Zhifanggou watershed

Communities of arbuscular mycorrhizal fungi and bacteria in the rhizosphere of Caragana korshinkii and Hippophae rhamnoides in Zhifanggou watershed

Bacterial community structure corresponds to performance during cathodic nitrate reduction

Hydrogenotrophic denitrification of potable water: A review

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